##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550538_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	605363
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25238906243031	33.0	31.0	34.0	30.0	34.0
2	32.27260833582495	34.0	31.0	34.0	30.0	34.0
3	32.25384603948375	34.0	31.0	34.0	30.0	34.0
4	35.90366110911965	37.0	35.0	37.0	35.0	37.0
5	35.973336659161525	37.0	35.0	37.0	35.0	37.0
6	35.92594856309355	37.0	35.0	37.0	35.0	37.0
7	36.200502838792595	37.0	36.0	37.0	35.0	37.0
8	36.04958182115524	37.0	36.0	37.0	35.0	37.0
9	37.90726720992198	39.0	38.0	39.0	35.0	39.0
10	37.52299364183143	39.0	37.0	39.0	35.0	39.0
11	37.474115530681594	39.0	37.0	39.0	35.0	39.0
12	37.44117496444282	39.0	37.0	39.0	35.0	39.0
13	37.452569780445785	39.0	37.0	39.0	35.0	39.0
14	38.67071327451463	40.0	38.0	41.0	35.0	41.0
15	38.787998275414914	40.0	38.0	41.0	35.0	41.0
16	38.545466108764494	40.0	38.0	41.0	34.0	41.0
17	38.60709524698404	40.0	38.0	41.0	35.0	41.0
18	38.50906315714703	40.0	38.0	41.0	34.0	41.0
19	38.368215434375735	40.0	37.0	41.0	34.0	41.0
20	38.525833260374355	40.0	38.0	41.0	35.0	41.0
21	38.428772488572974	40.0	37.0	41.0	34.0	41.0
22	38.52463232804119	40.0	38.0	41.0	35.0	41.0
23	38.530258704281565	40.0	38.0	41.0	35.0	41.0
24	38.472600737078416	40.0	38.0	41.0	35.0	41.0
25	38.22281176748496	40.0	37.0	41.0	34.0	41.0
26	38.31482598044479	40.0	37.0	41.0	34.0	41.0
27	38.32809405265932	40.0	37.0	41.0	34.0	41.0
28	38.24953457677459	40.0	37.0	41.0	34.0	41.0
29	38.231172040577306	40.0	37.0	41.0	34.0	41.0
30	38.06674507692079	40.0	37.0	41.0	34.0	41.0
31	38.02951287078992	40.0	37.0	41.0	34.0	41.0
32	37.98077351935946	40.0	37.0	41.0	34.0	41.0
33	37.896554629205944	40.0	37.0	41.0	34.0	41.0
34	37.80992065917474	40.0	37.0	41.0	33.0	41.0
35	37.7176239710719	40.0	37.0	41.0	33.0	41.0
36	37.58584188329977	40.0	36.0	41.0	33.0	41.0
37	37.54210779317533	40.0	36.0	41.0	33.0	41.0
38	37.44420950735344	40.0	36.0	41.0	33.0	41.0
39	37.378197874663634	40.0	36.0	41.0	33.0	41.0
40	37.28566661655899	40.0	36.0	41.0	33.0	41.0
41	37.160189836511314	40.0	35.0	41.0	32.0	41.0
42	37.12952889423371	40.0	35.0	41.0	32.0	41.0
43	37.066135525296396	40.0	35.0	41.0	32.0	41.0
44	36.95538709831952	40.0	35.0	41.0	32.0	41.0
45	36.949826798135994	39.0	35.0	41.0	32.0	41.0
46	36.820525866298404	39.0	35.0	41.0	32.0	41.0
47	36.76697948173245	39.0	35.0	41.0	31.0	41.0
48	36.655899022569926	39.0	35.0	41.0	31.0	41.0
49	36.58416189955448	39.0	35.0	41.0	31.0	41.0
50	36.47037562586415	39.0	35.0	41.0	31.0	41.0
51	35.36677167253367	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	7.0
9	15.0
10	10.0
11	14.0
12	9.0
13	12.0
14	17.0
15	24.0
16	31.0
17	66.0
18	139.0
19	251.0
20	368.0
21	624.0
22	868.0
23	1330.0
24	2048.0
25	3087.0
26	4326.0
27	5368.0
28	5735.0
29	6138.0
30	7088.0
31	8875.0
32	11429.0
33	16052.0
34	30524.0
35	42059.0
36	39945.0
37	61249.0
38	117539.0
39	239964.0
40	150.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.41100298498587	22.30215589654472	26.017447382809983	13.269393735659433
2	32.81997743502659	25.11451806601989	25.43449137129293	16.631013127660594
3	32.40171599519627	23.91969776811599	27.29684503347578	16.38174120321196
4	29.438204845687626	25.799561585362834	26.389951153274975	18.37228241567456
5	25.477936378668666	30.202539633244847	25.490160449185034	18.829363538901454
6	26.604698338021983	34.500126370458716	24.201181770276676	14.693993521242627
7	82.03953660861333	5.708806121285906	8.186162682555755	4.065494587544994
8	83.8386224463669	4.90053075592661	7.703642277443451	3.5572045202630487
9	76.54399096079543	8.455422614200076	9.898028125273596	5.102558299730905
10	37.89081922747178	32.54427508783986	16.530247140971614	13.034658543716745
11	27.914986545262927	24.441203046766983	29.020108595999428	18.62370181197067
12	26.57149511945725	22.788310484783512	30.508471776438267	20.131722619320968
13	24.179707051801977	24.06291762132803	31.344168705388338	20.413206621481656
14	20.811810434400517	27.527120091581413	30.420425430692	21.24064404332607
15	20.243556345531523	26.43636958320875	33.06462403549606	20.255450035763666
16	23.54306424409817	25.193644144092058	31.39636879029607	19.866922821513704
17	22.64740329356105	25.21842266540902	29.520799916744167	22.61337412428576
18	22.81887066107443	25.865142071781726	30.448342564709108	20.86764470243474
19	24.463999286378584	26.761133402603065	27.98056703168182	20.794300279336532
20	25.41582488523415	26.7110807895428	28.303844139797114	19.569250185425936
21	24.592682407084677	26.272335772090464	29.2761533162747	19.858828504550164
22	23.935721211900958	24.692952823347312	28.231821237835813	23.139504726915916
23	22.52119802498666	26.09508674960313	29.029854814384098	22.353860411026112
24	22.407547207212865	26.09194813690298	29.90602332815187	21.594481327732286
25	22.865289091008208	26.993225552271944	27.81471612900028	22.326769227719566
26	21.738692321797004	28.13204639199951	27.70849886762158	22.42076241858191
27	22.53887337019276	26.752873895497416	29.118066350272482	21.590186384037345
28	21.467780488731556	27.25819054022132	29.048686490584984	22.225342480462135
29	22.493941651538	25.67765126048338	29.24476718927321	22.583639898705403
30	22.643934300576678	25.31885827181377	29.389969324190613	22.64723810341894
31	23.575937082378672	26.748413761660363	27.31584189981879	22.359807256142183
32	23.51993762420234	27.058971228832952	27.42982309787681	21.991268049087903
33	22.411346580481464	26.691918733057683	27.54429986636118	23.352434820099678
34	21.81963549143241	26.983644524029383	28.852110221470422	22.34460976306778
35	22.627580476507482	25.58018907663666	28.69418844561032	23.098042001245535
36	22.673668526157034	27.099277623508538	28.351418900725676	21.875634949608745
37	22.74370914641298	27.70536025492143	27.929523277768876	21.62140732089672
38	22.085426430092358	27.079289616312856	27.886408650677364	22.948875302917422
39	22.536725898345292	25.825000867248242	28.688241600494248	22.950031633912214
40	22.8487700767969	25.7334855285176	29.733895200070044	21.683849194615462
41	21.369492354174273	26.34303715291486	29.35362749292573	22.93384299998513
42	22.31735338961912	27.09564344038205	28.256599759152774	22.330403410846053
43	22.949370873343764	25.1563111719745	28.72739166417505	23.166926290506687
44	22.186357606923448	25.86084712808678	28.347784717599193	23.605010547390574
45	22.404408594512716	25.258563869942495	28.363808161384163	23.973219374160628
46	22.544489835024606	26.536805189613506	28.26023394227926	22.65847103308263
47	21.921557809116184	26.361868829115753	28.813951298642305	22.90262206312576
48	21.61826870819657	25.604472027527287	28.94461670105375	23.832642563222397
49	22.24912986092642	25.23791510217836	29.592327248279133	22.920627788616084
50	21.360902466784392	25.01556917089416	29.339255950561892	24.284272411759556
51	21.245104177163125	24.725660471485703	28.68328589623086	25.345949455120316
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	370.0
1	670.0
2	970.0
3	3112.0
4	5254.0
5	3513.5
6	1773.0
7	1728.5
8	1684.0
9	1681.0
10	1678.0
11	1706.5
12	1735.0
13	1692.0
14	1649.0
15	1574.5
16	1500.0
17	1442.0
18	1384.0
19	1444.0
20	1504.0
21	1878.5
22	2253.0
23	2408.5
24	2564.0
25	3369.0
26	5371.0
27	6568.0
28	7507.0
29	8446.0
30	10027.0
31	11608.0
32	12731.5
33	13855.0
34	15421.5
35	16988.0
36	18582.5
37	20177.0
38	21848.5
39	23520.0
40	26430.5
41	29341.0
42	32072.0
43	34803.0
44	37378.5
45	39954.0
46	43919.5
47	47885.0
48	51719.0
49	55553.0
50	53383.0
51	51213.0
52	46421.0
53	41629.0
54	37513.5
55	33398.0
56	31745.5
57	30093.0
58	28133.0
59	26173.0
60	25494.0
61	24815.0
62	22633.0
63	20451.0
64	17079.0
65	13707.0
66	11530.5
67	9354.0
68	7561.0
69	5768.0
70	5049.0
71	4330.0
72	3547.5
73	2765.0
74	2345.5
75	1539.0
76	1152.0
77	892.5
78	633.0
79	509.0
80	385.0
81	325.0
82	265.0
83	170.0
84	75.0
85	51.0
86	27.0
87	16.5
88	6.0
89	5.0
90	4.0
91	3.0
92	2.0
93	1.0
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	605363.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.77859645036244
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.44386276891352	21.274979582505495
2	8.369057148681348	4.984375510012056
3	3.2033547318048012	2.8617422353732254
4	1.8380901791459792	2.1894298273665007
5	1.3233475412260784	1.970371619687538
6	1.1302081156730661	2.019360682893167
7	0.9707492239644686	2.023528457645829
8	0.8887832849074705	2.1173375018469653
9	0.835202787372665	2.238405008344961
>10	9.889123875609112	53.86175069208077
>50	0.06805609180193084	1.3656394296384136
>100	0.03625939317315861	1.9923950029294115
>500	0.0033470209082915644	0.6081505841024325
>1k	5.578368180485941E-4	0.49253386557326206
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2965	0.48978877136528	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	805	0.1329780644010288	No Hit
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC	646	0.1067128318050492	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6519014211307926E-4	0.0	0.0	0.09234128944121131	0.0
2	1.6519014211307926E-4	0.0	0.0	0.3753120028809161	0.0
3	1.6519014211307926E-4	0.0	0.0	0.5965016031703292	0.0
4	1.6519014211307926E-4	0.0	0.0	0.8821153588838433	0.0
5	1.6519014211307926E-4	0.0	0.0	1.7121958230020666	0.0
6	1.6519014211307926E-4	0.0	0.0	2.40764632129813	0.0
7	1.6519014211307926E-4	0.0	0.0	2.99935741034718	0.0
8	1.6519014211307926E-4	0.0	0.0	3.8380277618552836	0.0
9	1.6519014211307926E-4	0.0	0.0	4.238448666337388	0.0
10	1.6519014211307926E-4	0.0	0.0	4.941497911170653	0.0
11	1.6519014211307926E-4	0.0	0.0	5.89893997485806	0.0
12	1.6519014211307926E-4	0.0	0.0	6.670543128668253	0.0
13	1.6519014211307926E-4	0.0	0.0	6.9878733916674785	0.0
14	1.6519014211307926E-4	0.0	0.0	7.126467920900352	0.0
15	1.6519014211307926E-4	0.0	0.0	7.3418758662158075	0.0
16	1.6519014211307926E-4	0.0	0.0	7.7816120245208245	0.0
17	1.6519014211307926E-4	0.0	0.0	8.352839535947853	0.0
18	1.6519014211307926E-4	0.0	0.0	8.938934160165058	0.0
19	1.6519014211307926E-4	0.0	0.0	9.285172698034073	0.0
20	1.6519014211307926E-4	0.0	0.0	9.64231378528255	0.0
21	1.6519014211307926E-4	0.0	0.0	10.057106232128492	0.0
22	1.6519014211307926E-4	0.0	0.0	10.51732596805553	0.0
23	3.303802842261585E-4	0.0	0.0	10.982666598388075	0.0
24	3.303802842261585E-4	0.0	0.0	11.363099495674495	0.0
25	3.303802842261585E-4	0.0	0.0	11.678282286826251	0.0
26	3.303802842261585E-4	0.0	0.0	11.986857472293483	0.0
27	4.955704263392378E-4	0.0	0.0	12.304848495861162	0.0
28	4.955704263392378E-4	0.0	0.0	12.62184837857616	0.0
29	4.955704263392378E-4	0.0	0.0	12.964452733318687	0.0
30	4.955704263392378E-4	0.0	0.0	13.35463184898978	0.0
31	4.955704263392378E-4	0.0	0.0	13.698227344584984	0.0
32	4.955704263392378E-4	0.0	0.0	14.04182284018019	0.0
33	6.60760568452317E-4	0.0	0.0	14.371377173695782	0.0
34	6.60760568452317E-4	0.0	0.0	14.703904929769411	0.0
35	6.60760568452317E-4	0.0	0.0	15.071783376255238	0.0
36	6.60760568452317E-4	0.0	0.0	15.440322583309518	0.0
37	6.60760568452317E-4	0.0	0.0	15.788873783168116	0.0
38	8.259507105653963E-4	0.0	0.0	16.14089397601109	0.0
39	8.259507105653963E-4	0.0	0.0	16.505138239370428	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTTA	25	3.8886104E-5	45.0	38
TTTCGCG	20	7.0306385E-4	45.0	1
CATGACG	20	7.0306385E-4	45.0	1
TAACGCC	20	7.0306385E-4	45.0	12
CTTGACG	20	7.0306385E-4	45.0	1
TGTAGCG	40	6.8066583E-9	45.0	1
TAGTGCG	40	6.8066583E-9	45.0	1
TGCGTTG	20	7.0306385E-4	45.0	1
CGACTGG	30	2.1637934E-6	44.999996	2
CGTTTTT	1495	0.0	39.431435	1
GATTACG	40	3.4546065E-7	39.375	1
CGGGTAT	75	0.0	39.0	6
GCTACGA	105	0.0	38.57143	10
CGCGGGT	35	6.24339E-6	38.571426	4
CCGCAGG	35	6.24339E-6	38.571426	2
ACGGGTA	65	9.094947E-12	38.076927	5
CACGACA	30	1.1392839E-4	37.499996	34
TAACGAG	30	1.1392839E-4	37.499996	1
ATGCGAG	30	1.1392839E-4	37.499996	1
TATCGCG	30	1.1392839E-4	37.499996	1
>>END_MODULE