FastQCFastQC Report
Sat 18 Jun 2016
SRR3550536_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550536_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences495234
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14870.30026209832119766No Hit
GAATCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTC13830.2792619246659155No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCG13180.26613681613136414No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGC11540.23302115767495768No Hit
GCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC8260.16678984076214476No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAACG207.029393E-445.01
CGGATAT207.029393E-445.031
GTATCGG253.887579E-545.02
TATAGCG253.887579E-545.01
TCGACGT253.887579E-545.026
TAGTACG253.887579E-545.01
GTACGAG253.887579E-545.01
TTAGCGG900.042.52
CGTAAGG1100.040.9090922
GTTAGCG451.9244908E-840.01
GCGTAAG451.9244908E-840.01
GCCGAAC403.4529148E-739.37542
ACGGTCG403.4529148E-739.37511
CGATAGT356.241091E-638.57142610
TCGAAAG356.241091E-638.5714261
AGAGACG356.241091E-638.5714261
CTACGAA900.037.511
CGACGTT301.1389839E-437.49999627
GTAATAC301.1389839E-437.49999622
CGATGTA301.1389839E-437.49999610