##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550536_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	495234
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.23863264638535	33.0	31.0	34.0	30.0	34.0
2	32.2539991196081	34.0	31.0	34.0	30.0	34.0
3	32.251584099637746	34.0	31.0	34.0	30.0	34.0
4	35.89770492332917	37.0	35.0	37.0	35.0	37.0
5	35.9637464309801	37.0	35.0	37.0	35.0	37.0
6	35.9069328842527	37.0	35.0	37.0	35.0	37.0
7	36.16331471587169	37.0	35.0	37.0	35.0	37.0
8	35.980053873522415	37.0	35.0	37.0	35.0	37.0
9	37.829559359817786	39.0	38.0	39.0	35.0	39.0
10	37.47025042707084	39.0	37.0	39.0	35.0	39.0
11	37.44164980595032	39.0	37.0	39.0	35.0	39.0
12	37.35935941393362	39.0	37.0	39.0	35.0	39.0
13	37.326706163147115	39.0	37.0	39.0	34.0	39.0
14	38.51255164225397	40.0	38.0	41.0	34.0	41.0
15	38.620223974929026	40.0	38.0	41.0	35.0	41.0
16	38.421764256896736	40.0	38.0	41.0	34.0	41.0
17	38.45499905095369	40.0	38.0	41.0	34.0	41.0
18	38.384373851553	40.0	37.0	41.0	34.0	41.0
19	38.21829478589919	40.0	37.0	41.0	34.0	41.0
20	38.361473566031414	40.0	37.0	41.0	34.0	41.0
21	38.280840976185	40.0	37.0	41.0	34.0	41.0
22	38.36753534692691	40.0	37.0	41.0	34.0	41.0
23	38.37728225444941	40.0	37.0	41.0	34.0	41.0
24	38.30110008601994	40.0	37.0	41.0	34.0	41.0
25	38.05546670866701	40.0	37.0	41.0	34.0	41.0
26	38.132377825432016	40.0	37.0	41.0	34.0	41.0
27	38.130934063493214	40.0	37.0	41.0	34.0	41.0
28	38.02632290997791	40.0	36.0	41.0	34.0	41.0
29	38.008046701155415	40.0	36.0	41.0	34.0	41.0
30	37.8602499020665	40.0	36.0	41.0	33.0	41.0
31	37.81992149165849	40.0	36.0	41.0	33.0	41.0
32	37.756190003109644	40.0	36.0	41.0	33.0	41.0
33	37.683638441625575	40.0	36.0	41.0	33.0	41.0
34	37.58225606480977	40.0	36.0	41.0	33.0	41.0
35	37.48867807945335	40.0	36.0	41.0	33.0	41.0
36	37.37706619497046	40.0	35.0	41.0	33.0	41.0
37	37.31217969687057	40.0	35.0	41.0	33.0	41.0
38	37.19785596303969	40.0	35.0	41.0	33.0	41.0
39	37.131000698659626	40.0	35.0	41.0	32.0	41.0
40	37.04045158450349	39.0	35.0	41.0	32.0	41.0
41	36.89674981927735	39.0	35.0	41.0	32.0	41.0
42	36.889936878324185	39.0	35.0	41.0	32.0	41.0
43	36.81716319961877	39.0	35.0	41.0	32.0	41.0
44	36.69999636535456	39.0	35.0	41.0	31.0	41.0
45	36.661723548867805	39.0	35.0	41.0	31.0	41.0
46	36.542117463663644	39.0	35.0	41.0	31.0	41.0
47	36.471686112019775	39.0	35.0	41.0	31.0	41.0
48	36.35569246053381	39.0	35.0	41.0	31.0	41.0
49	36.28630102133537	38.0	35.0	41.0	31.0	41.0
50	36.18721452888937	38.0	35.0	40.0	31.0	41.0
51	35.019475641817806	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	5.0
9	5.0
10	9.0
11	5.0
12	7.0
13	13.0
14	25.0
15	22.0
16	31.0
17	70.0
18	118.0
19	227.0
20	438.0
21	650.0
22	962.0
23	1396.0
24	1965.0
25	2586.0
26	3476.0
27	4013.0
28	4489.0
29	5194.0
30	6280.0
31	7692.0
32	10238.0
33	14319.0
34	28502.0
35	38492.0
36	35136.0
37	53444.0
38	96314.0
39	178995.0
40	114.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.94979746947907	20.359668358796043	24.37797081783561	12.312563353889272
2	33.05144638696051	25.963282004062727	24.180084566083913	16.805187042892854
3	32.769357515840994	24.674800195463153	25.963282004062727	16.592560284633123
4	29.262328515408875	25.559432510691916	26.46506499957596	18.71317397432325
5	26.661335853354174	30.6012915106798	23.51918487018258	19.218187765783448
6	26.609239268709338	36.18491460602463	22.856064002067708	14.349782123198326
7	80.77938913725633	6.4676496363335305	8.617946263786411	4.135014962623729
8	82.01456281273096	6.619295121094271	7.384387986285271	3.9817540798895066
9	75.5707402965063	8.24882782684549	9.188585597919367	6.991846278728843
10	41.682517759281474	26.842664275877663	16.331471587168895	15.143346377671968
11	32.630231365374755	25.571144145999668	22.680995246691463	19.117629241934118
12	29.52806148204687	22.923103017967264	27.063368024004813	20.48546747598105
13	26.326140773856398	23.92303436355339	28.94308549089925	20.807739371690957
14	21.510235565409484	28.244627792114436	28.34760941292399	21.89752722955209
15	21.23844485637093	25.6149618160304	32.30614214694468	20.840451180653993
16	23.722119240601415	23.536550398397523	30.98151580868842	21.759814552312644
17	24.048025781751655	24.170796027736383	28.21433908011162	23.566839110400338
18	24.55182802473174	25.159015738014755	27.272158212077525	23.016998025175976
19	26.54139255382304	26.199332032937967	25.691693219770855	21.567582193468137
20	27.98333716990352	25.393450368916515	26.241736229741903	20.381476231438068
21	26.65184539025996	26.401862553863424	26.167225998214988	20.77906605766163
22	24.30507598428218	24.879148039108784	26.618527807056868	24.19724816955217
23	25.11398651950391	26.72837486925373	26.041023031536607	22.116615579705755
24	24.513058473368147	25.49037424732551	27.441774999293262	22.554792280013082
25	23.744532887483494	26.327352322336512	26.436391685546628	23.491723104633365
26	22.88332384287024	27.704277169984294	26.49939220651248	22.913006780632994
27	24.734166070988664	26.959174854715144	26.41034339322421	21.89631568107198
28	22.577004002148477	27.204917271431285	27.99141415977094	22.2266645666493
29	23.606416360750675	26.306352148681228	27.590795462347096	22.496436028221005
30	24.914888719272103	25.813049992528786	26.924645723031944	22.34741556516717
31	25.90230073056373	26.026484449775257	25.03624549203003	23.03496932763098
32	26.11432171458341	26.50585379840641	25.82455970308985	21.55526478392033
33	26.496969109552253	25.96408970304947	25.561047908665397	21.977893278732882
34	24.754560470403888	26.189841569843754	27.0096560413865	22.045941918365862
35	25.09742869027571	24.164738285335822	26.74452884898856	23.993304175399913
36	25.613548342803604	26.663960875061083	25.60708675090967	22.115404031225644
37	25.327420976750386	27.26206197474325	26.77400986200463	20.636507186501735
38	25.14932335017386	26.260919080677013	25.546711251650734	23.043046317498394
39	25.91158926891126	24.59625146900253	26.531296316488774	22.960862945597434
40	25.02877427640267	24.43370204792078	28.29531090353247	22.242212772144075
41	22.53944599926499	25.551557445571184	28.41969654749068	23.48930000767314
42	23.173489703857168	26.640133754952206	27.45550588206787	22.730870659122758
43	24.695598444371754	24.803628183848446	27.64814209040575	22.85263128137406
44	24.460759963976624	25.89664683765654	26.499190281765795	23.14340291660104
45	23.97573672243828	25.352459645339376	26.178331859282682	24.49347177293966
46	23.549675506932076	26.68738414567659	26.407516446770618	23.355423900620718
47	23.871947402641982	25.603654030216017	28.373455780499725	22.150942786642275
48	23.769369631325798	24.839166939265077	27.671565361021255	23.719898068387874
49	22.56610006582747	25.172140846549308	28.617178949749007	23.644580137874215
50	22.604869617191063	24.961937185249802	28.160223247999937	24.272969949559197
51	22.51016691099561	24.95810061506278	26.78774074477924	25.743991729162374
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	249.0
1	597.0
2	945.0
3	1907.0
4	2869.0
5	1994.0
6	1119.0
7	1116.0
8	1113.0
9	1105.5
10	1098.0
11	1167.0
12	1236.0
13	1215.0
14	1194.0
15	1194.5
16	1195.0
17	1143.5
18	1092.0
19	1255.5
20	1419.0
21	1446.0
22	1473.0
23	1630.0
24	1787.0
25	2223.0
26	3294.0
27	3929.0
28	4716.0
29	5503.0
30	6411.5
31	7320.0
32	8470.5
33	9621.0
34	10975.5
35	12330.0
36	12829.0
37	13328.0
38	14179.5
39	15031.0
40	17481.0
41	19931.0
42	22052.0
43	24173.0
44	26234.5
45	28296.0
46	30339.5
47	32383.0
48	37309.0
49	42235.0
50	41028.5
51	39822.0
52	39153.5
53	38485.0
54	35237.5
55	31990.0
56	30278.0
57	28566.0
58	27059.5
59	25553.0
60	25827.5
61	26102.0
62	24311.0
63	22520.0
64	19380.5
65	16241.0
66	13656.0
67	11071.0
68	9415.0
69	7759.0
70	6408.5
71	5058.0
72	4380.0
73	3702.0
74	2997.5
75	1771.0
76	1249.0
77	959.5
78	670.0
79	507.5
80	345.0
81	242.5
82	140.0
83	101.0
84	62.0
85	49.0
86	36.0
87	30.5
88	25.0
89	15.0
90	5.0
91	5.5
92	6.0
93	5.0
94	4.0
95	2.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	495234.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.216876377182327
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.25270189590817	20.817813828329744
2	8.035351690424843	4.695357539726514
3	3.0955932422559864	2.713306952191026
4	1.8691334629220668	2.1844096527459493
5	1.3789608289282123	2.0144464033886225
6	1.192132410966715	2.0898231165868095
7	1.0576267921483447	2.1630385867575863
8	0.9503189400983549	2.2212280793398858
9	0.8576722645141553	2.255265407200328
>10	10.20030049472578	54.01115842785613
>50	0.07000632508076296	1.3957092549330532
>100	0.03673599236911323	2.1893518216890944
>500	6.931319314927026E-4	0.16727449863242919
>1k	0.0027725277259708103	1.0818164306228046
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1487	0.30026209832119766	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTC	1383	0.2792619246659155	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCG	1318	0.26613681613136414	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGC	1154	0.23302115767495768	No Hit
GCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC	826	0.16678984076214476	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.038494933708106E-4	0.0	0.0	0.09086613600843238	0.0
2	4.038494933708106E-4	0.0	0.0	0.42888816195980084	0.0
3	4.038494933708106E-4	0.0	0.0	0.6158704773904861	0.0
4	4.038494933708106E-4	0.0	0.0	0.8769591748547152	0.0
5	4.038494933708106E-4	0.0	0.0	1.735341273014373	0.0
6	4.038494933708106E-4	0.0	0.0	2.267816830023787	0.0
7	4.038494933708106E-4	0.0	0.0	2.7326475968935897	0.0
8	4.038494933708106E-4	0.0	0.0	3.397787712475315	0.0
9	4.038494933708106E-4	0.0	0.0	3.712386467811176	0.0
10	4.038494933708106E-4	0.0	0.0	4.581268652798475	0.0
11	4.038494933708106E-4	0.0	0.0	5.443891170638526	0.0
12	6.057742400562158E-4	0.0	0.0	6.332158131307624	0.0
13	6.057742400562158E-4	0.0	0.0	6.62373746552135	0.0
14	6.057742400562158E-4	0.0	0.0	6.7693252078815265	0.0
15	6.057742400562158E-4	0.0	0.0	7.012240678144069	0.0
16	6.057742400562158E-4	0.0	0.0	7.420128666448588	0.0
17	6.057742400562158E-4	0.0	0.0	7.863353485423053	0.0
18	8.076989867416212E-4	0.0	0.0	8.355646017842071	0.0
19	8.076989867416212E-4	0.0	0.0	8.703562356381024	0.0
20	8.076989867416212E-4	0.0	0.0	9.031286220251436	0.0
21	8.076989867416212E-4	0.0	0.0	9.398183484978818	0.0
22	8.076989867416212E-4	0.0	0.0	9.804254150563168	0.0
23	8.076989867416212E-4	0.0	0.0	10.188315018758809	0.0
24	8.076989867416212E-4	0.0	0.0	10.50816381750849	0.0
25	8.076989867416212E-4	0.0	0.0	10.782175698760586	0.0
26	8.076989867416212E-4	0.0	0.0	11.048918289132006	0.0
27	8.076989867416212E-4	0.0	0.0	11.311622384569718	0.0
28	8.076989867416212E-4	0.0	0.0	11.591288158729006	0.0
29	0.0010096237334270264	0.0	0.0	11.875194352568684	0.0
30	0.0010096237334270264	0.0	0.0	12.23663964913556	0.0
31	0.0010096237334270264	0.0	0.0	12.560325018072264	0.0
32	0.0010096237334270264	0.0	0.0	12.873510300181328	0.0
33	0.0010096237334270264	0.0	0.0	13.153781848580671	0.0
34	0.0010096237334270264	0.0	0.0	13.436274569193554	0.0
35	0.0010096237334270264	0.0	0.0	13.749661776049303	0.0
36	0.0012115484801124316	0.0	0.0	14.052952745570781	0.0
37	0.0012115484801124316	0.0	0.0	14.387743975575182	0.0
38	0.0012115484801124316	0.0	0.0	14.69022724610992	0.0
39	0.0012115484801124316	0.0	0.0	14.995739387844939	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTAACG	20	7.029393E-4	45.0	1
CGGATAT	20	7.029393E-4	45.0	31
GTATCGG	25	3.887579E-5	45.0	2
TATAGCG	25	3.887579E-5	45.0	1
TCGACGT	25	3.887579E-5	45.0	26
TAGTACG	25	3.887579E-5	45.0	1
GTACGAG	25	3.887579E-5	45.0	1
TTAGCGG	90	0.0	42.5	2
CGTAAGG	110	0.0	40.909092	2
GTTAGCG	45	1.9244908E-8	40.0	1
GCGTAAG	45	1.9244908E-8	40.0	1
GCCGAAC	40	3.4529148E-7	39.375	42
ACGGTCG	40	3.4529148E-7	39.375	11
CGATAGT	35	6.241091E-6	38.571426	10
TCGAAAG	35	6.241091E-6	38.571426	1
AGAGACG	35	6.241091E-6	38.571426	1
CTACGAA	90	0.0	37.5	11
CGACGTT	30	1.1389839E-4	37.499996	27
GTAATAC	30	1.1389839E-4	37.499996	22
CGATGTA	30	1.1389839E-4	37.499996	10
>>END_MODULE