Basic Statistics
Measure | Value |
---|---|
Filename | SRR3550535_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 712121 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 47 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 4993 | 0.701144889702733 | No Hit |
GAATCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTC | 1116 | 0.15671494029806732 | No Hit |
CCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC | 1015 | 0.14253195734994473 | Illumina Single End Adapter 1 (95% over 22bp) |
CGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG | 1007 | 0.14140855275999445 | Illumina Single End Adapter 1 (95% over 21bp) |
CGTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTT | 977 | 0.1371957855476808 | No Hit |
GCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC | 922 | 0.12947237899177247 | Illumina Single End Adapter 1 (95% over 22bp) |
CGTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTC | 802 | 0.1126213101425179 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ATTCGCG | 20 | 7.031476E-4 | 45.000004 | 1 |
TCTACGG | 40 | 6.8084773E-9 | 45.000004 | 2 |
ACCGGGT | 25 | 3.889306E-5 | 45.0 | 4 |
CGTTGAT | 25 | 3.889306E-5 | 45.0 | 25 |
CGTTTTT | 2670 | 0.0 | 41.544945 | 1 |
TAGCCGT | 50 | 1.0804797E-9 | 40.5 | 44 |
CTCGCGG | 50 | 1.0804797E-9 | 40.5 | 2 |
ACACGAC | 205 | 0.0 | 39.512196 | 26 |
CGAGACA | 200 | 0.0 | 39.375 | 22 |
CACGACC | 210 | 0.0 | 38.57143 | 27 |
GTACTAG | 65 | 9.094947E-12 | 38.07692 | 1 |
TAAGGGA | 635 | 0.0 | 37.913387 | 4 |
CGTAAGG | 95 | 0.0 | 37.894737 | 2 |
TCTAGCG | 30 | 1.1394858E-4 | 37.499996 | 1 |
CTAACGG | 60 | 1.546141E-10 | 37.499996 | 2 |
CGAATAT | 120 | 0.0 | 37.499996 | 14 |
GCCACTA | 30 | 1.1394858E-4 | 37.499996 | 36 |
TTACACG | 355 | 0.0 | 37.394367 | 34 |
TAGTGCG | 55 | 2.746674E-9 | 36.818184 | 1 |
GCGTATG | 55 | 2.746674E-9 | 36.818184 | 1 |