FastQCFastQC Report
Sat 18 Jun 2016
SRR3550535_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550535_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences712121
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49930.701144889702733No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTC11160.15671494029806732No Hit
CCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC10150.14253195734994473Illumina Single End Adapter 1 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG10070.14140855275999445Illumina Single End Adapter 1 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTT9770.1371957855476808No Hit
GCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC9220.12947237899177247Illumina Single End Adapter 1 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTC8020.1126213101425179No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGCG207.031476E-445.0000041
TCTACGG406.8084773E-945.0000042
ACCGGGT253.889306E-545.04
CGTTGAT253.889306E-545.025
CGTTTTT26700.041.5449451
TAGCCGT501.0804797E-940.544
CTCGCGG501.0804797E-940.52
ACACGAC2050.039.51219626
CGAGACA2000.039.37522
CACGACC2100.038.5714327
GTACTAG659.094947E-1238.076921
TAAGGGA6350.037.9133874
CGTAAGG950.037.8947372
TCTAGCG301.1394858E-437.4999961
CTAACGG601.546141E-1037.4999962
CGAATAT1200.037.49999614
GCCACTA301.1394858E-437.49999636
TTACACG3550.037.39436734
TAGTGCG552.746674E-936.8181841
GCGTATG552.746674E-936.8181841