FastQCFastQC Report
Sat 18 Jun 2016
SRR3550534_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550534_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences546169
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11850.21696581094862577No Hit
GAATCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTC11810.21623343690322958No Hit
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC11670.21367012774434288No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT9210.16862912395247626No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGC8870.1624039445666085No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCG8770.16057300945311798No Hit
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC7580.1387848816025809No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT7530.13786941404583564No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAAT207.0300297E-445.00000420
ATGTACG207.0300297E-445.0000041
TCATCGA207.0300297E-445.00000416
CGGTCTA253.888107E-545.00000431
TCGACAA207.0300297E-445.00000419
GCGTACG253.888107E-545.0000041
AGGGATC2150.041.8604626
CGAATAT1200.041.25000414
GACGTAG556.002665E-1140.9090921
TACGGGT501.0786607E-940.5000044
TAGGGAT3050.039.8360675
TACGAAT1250.039.612
ACGGGAT2050.039.5121965
GTAGGGA4450.039.43824
ATAGGGT750.039.04
ACAACGA1850.038.9189213
ACGGGTA700.038.5714265
AATATAC1350.038.33333216
CGGGTAC659.094947E-1238.0769276
ATAGGGC1950.038.0769234