##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550534_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	546169
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.157011840657376	33.0	31.0	34.0	30.0	34.0
2	32.16910882895221	33.0	31.0	34.0	30.0	34.0
3	32.176542425512984	34.0	31.0	34.0	30.0	34.0
4	35.83209043354712	37.0	35.0	37.0	35.0	37.0
5	35.906624140147095	37.0	35.0	37.0	35.0	37.0
6	35.85641989933519	37.0	35.0	37.0	35.0	37.0
7	36.12752829252484	37.0	35.0	37.0	35.0	37.0
8	35.94263314102412	37.0	35.0	37.0	35.0	37.0
9	37.77256490207244	39.0	38.0	39.0	35.0	39.0
10	37.405645505328934	39.0	37.0	39.0	34.0	39.0
11	37.36035000155629	39.0	37.0	39.0	35.0	39.0
12	37.168975170688924	39.0	37.0	39.0	34.0	39.0
13	37.08322149371348	39.0	37.0	39.0	33.0	39.0
14	38.15923093401493	40.0	37.0	41.0	33.0	41.0
15	38.273955497291134	40.0	37.0	41.0	34.0	41.0
16	38.069551732156164	40.0	36.0	41.0	33.0	41.0
17	38.09079057947265	40.0	37.0	41.0	34.0	41.0
18	38.03147743647113	40.0	36.0	41.0	34.0	41.0
19	37.90086035640983	40.0	36.0	41.0	34.0	41.0
20	38.09634197473676	40.0	36.0	41.0	34.0	41.0
21	38.002422327155145	40.0	36.0	41.0	34.0	41.0
22	38.109632732725586	40.0	36.0	41.0	34.0	41.0
23	38.14082088144878	40.0	36.0	41.0	34.0	41.0
24	38.08921231340482	40.0	36.0	41.0	34.0	41.0
25	37.83921460207372	40.0	35.0	41.0	34.0	41.0
26	37.923776340290274	40.0	36.0	41.0	34.0	41.0
27	37.946567820583006	40.0	36.0	41.0	34.0	41.0
28	37.84752521655385	40.0	35.0	41.0	34.0	41.0
29	37.807949554075755	40.0	35.0	41.0	34.0	41.0
30	37.652911461470715	40.0	35.0	41.0	33.0	41.0
31	37.600504605717276	40.0	35.0	41.0	33.0	41.0
32	37.554723904139564	40.0	35.0	41.0	33.0	41.0
33	37.50154988657357	40.0	35.0	41.0	33.0	41.0
34	37.337745276645144	40.0	35.0	41.0	33.0	41.0
35	37.26947519906842	40.0	35.0	41.0	33.0	41.0
36	37.21204242642845	40.0	35.0	41.0	33.0	41.0
37	37.21625723905971	39.0	35.0	41.0	33.0	41.0
38	37.08905851485529	39.0	35.0	41.0	33.0	41.0
39	37.0219455882703	39.0	35.0	41.0	32.0	41.0
40	36.91050755352281	39.0	35.0	41.0	32.0	41.0
41	36.7908284798295	39.0	35.0	41.0	32.0	41.0
42	36.81545272617084	39.0	35.0	41.0	32.0	41.0
43	36.74335233233669	39.0	35.0	41.0	32.0	41.0
44	36.62635374764954	39.0	35.0	41.0	31.0	41.0
45	36.572842838022666	39.0	35.0	41.0	31.0	41.0
46	36.43735180868925	38.0	35.0	41.0	31.0	41.0
47	36.35627433999367	38.0	35.0	41.0	31.0	41.0
48	36.28730484520359	38.0	35.0	40.0	31.0	41.0
49	36.251533865891325	38.0	35.0	40.0	31.0	41.0
50	36.13499301498254	38.0	35.0	40.0	31.0	41.0
51	35.01393341621366	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	13.0
9	12.0
10	13.0
11	15.0
12	12.0
13	16.0
14	16.0
15	25.0
16	38.0
17	80.0
18	148.0
19	224.0
20	382.0
21	671.0
22	976.0
23	1439.0
24	2027.0
25	2877.0
26	3644.0
27	4374.0
28	5008.0
29	5861.0
30	7179.0
31	9474.0
32	12648.0
33	18024.0
34	38632.0
35	50208.0
36	37696.0
37	57472.0
38	104688.0
39	182177.0
40	99.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.130662853439134	20.753466417903617	24.258974786192553	10.856895942464694
2	32.85576442456456	24.473743475004987	24.917379053003742	17.753113047426712
3	32.541575959089585	25.01405242699604	25.580726844621353	16.86364476929302
4	28.817087751227184	25.987560626838945	25.76436963650445	19.43098198542942
5	26.2762990942364	29.974788023487235	23.941673731024647	19.807239151251718
6	26.71682208254222	34.54443587973686	23.736975185336405	15.001766852384518
7	80.4201629898438	6.419990881943134	8.392091092683767	4.767755035529296
8	81.17945178140832	5.865217542555509	8.102620251240916	4.852710424795256
9	75.16208353092175	7.686082512921824	10.23822296761625	6.913610988540177
10	42.561551461177764	25.697357411350698	16.603835076688718	15.137256050782815
11	34.17513626734582	24.817776182829856	22.374942554410815	18.63214499541351
12	32.0474065719585	21.504516001457425	26.64028899479831	19.807788431785767
13	27.15057061092812	24.306762192654656	27.62551517936756	20.917152017049666
14	21.214495879480527	27.28313031314483	29.180162184232355	22.322211623142287
15	21.631765991845015	25.045178323925377	32.8768201783697	20.446235505859907
16	25.662020363660332	24.04457228440281	30.20328872565085	20.090118626286003
17	23.70768022352056	24.319761831960438	28.33903059309481	23.633527351424195
18	26.08002285007022	25.074107098718528	27.511997202331145	21.33387284888011
19	26.37663433845568	27.938971270797136	24.733553167609294	20.950841223137893
20	28.87348055272269	26.257623556078798	25.352958516503133	19.515937374695376
21	26.303030746893363	27.745807616323887	25.79641100099054	20.15475063579222
22	23.834197839862753	25.042248827743798	26.92811199463902	24.19544133775443
23	24.93111106635492	26.303580027427408	27.046939683504554	21.718369222713115
24	25.538615337011073	23.278325939407033	27.919929545616835	23.263129177965062
25	23.79208633225247	25.773158125049207	25.837240854021378	24.597514688676945
26	21.9433911481611	26.56613612270195	27.169063055574373	24.321409673562577
27	22.696637853851097	26.22246960189978	26.220089386252244	24.860803157996884
28	20.867716768985424	28.367226993842564	27.03284148313068	23.73221475404133
29	23.97554603062422	27.428689654667327	25.83687466699868	22.758889647709772
30	24.81502978015962	25.13892220173609	26.929210555707115	23.116837462397168
31	26.92811199463902	26.42295699682699	24.488207862401566	22.160723146132426
32	27.02551774267672	27.473730658459196	23.916956106992522	21.583795491871562
33	26.670682517682252	26.172851260324187	24.273805360611824	22.882660861381733
34	23.76919964333384	26.45811095100601	28.310834192347055	21.461855213313093
35	21.99392495729344	27.579009427484902	26.435041168576028	23.992024446645637
36	22.801001155320055	30.005364639882526	25.073191631161784	22.120442573635632
37	24.306212912120607	28.168387440517495	25.980053792873637	21.54534585448826
38	24.01472804205292	28.855720482121832	23.844084889475603	23.285466586349646
39	25.438646279814492	25.173893062403764	26.537756628442843	22.849704029338906
40	24.408379091453376	23.748326983040048	28.35257951293464	23.490714412571933
41	20.263691275044906	26.527686485318647	27.201470607083156	26.007151632553295
42	22.529839665012112	25.35588801268472	26.570530366974328	25.543741955328848
43	23.43120902138349	24.53929095206795	26.910168830526814	25.119331196021744
44	22.903533521675527	25.06806501284401	26.655668849751635	25.372732615728832
45	23.440180603439593	25.288326506996917	25.670442665182392	25.6010502243811
46	23.23255256156977	26.52475698913706	25.894732216584977	24.34795823270819
47	21.45563003392723	25.529094474420923	28.84052372067986	24.174751770971987
48	21.925447984048894	24.425956068542888	28.57503812922374	25.073557818184483
49	22.721355477883222	24.051712931345428	28.74055466348328	24.486376927288074
50	20.982882587623976	23.971334879863193	28.60030503378991	26.445477498722923
51	20.524233341694604	23.90999855356126	27.932379904388565	27.63338820035557
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	286.0
1	634.5
2	983.0
3	1751.0
4	2519.0
5	1786.0
6	1053.0
7	1062.5
8	1072.0
9	1063.0
10	1054.0
11	1052.5
12	1051.0
13	1068.0
14	1085.0
15	1131.0
16	1177.0
17	1134.0
18	1091.0
19	1116.5
20	1142.0
21	1209.5
22	1277.0
23	1508.5
24	1740.0
25	2052.0
26	3051.5
27	3739.0
28	4366.5
29	4994.0
30	6132.5
31	7271.0
32	7972.0
33	8673.0
34	10561.0
35	12449.0
36	13527.5
37	14606.0
38	15728.0
39	16850.0
40	19031.0
41	21212.0
42	24283.0
43	27354.0
44	29910.0
45	32466.0
46	38662.5
47	44859.0
48	46992.5
49	49126.0
50	48263.5
51	47401.0
52	43553.5
53	39706.0
54	36994.0
55	34282.0
56	32831.0
57	31380.0
58	31273.0
59	31166.0
60	30070.5
61	28975.0
62	26541.0
63	24107.0
64	20295.0
65	16483.0
66	13660.0
67	10837.0
68	8860.0
69	6883.0
70	5727.5
71	4572.0
72	3965.5
73	3359.0
74	2919.0
75	1974.5
76	1470.0
77	1148.5
78	827.0
79	614.0
80	401.0
81	316.5
82	232.0
83	151.5
84	71.0
85	42.0
86	13.0
87	11.5
88	10.0
89	11.5
90	13.0
91	9.0
92	5.0
93	3.5
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	546169.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.139763344922233
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.4624587596446	21.840013584068753
2	9.470655966505118	5.708866591032801
3	3.223564255191172	2.914723913358373
4	1.8115683590465836	2.1840096649925256
5	1.1965409487461116	1.8031730513858264
6	0.9122511850248318	1.6497020896663976
7	0.7706802859636425	1.625968500347882
8	0.6894068864655193	1.6622848325144337
9	0.6184810874131461	1.6776786247148143
>10	8.674375184826811	52.88803712491542
>50	0.1206572834127594	2.4493665214110165
>100	0.044484756005714325	2.176622873818383
>500	0.003046901096281803	0.7706615119856498
>1k	0.0018281406577690818	0.6488911157877266
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1185	0.21696581094862577	No Hit
GAATCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTC	1181	0.21623343690322958	No Hit
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	1167	0.21367012774434288	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	921	0.16862912395247626	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGC	887	0.1624039445666085	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCG	877	0.16057300945311798	No Hit
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	758	0.1387848816025809	No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT	753	0.13786941404583564	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.661870226981026E-4	0.0	0.0	0.14281293885226	0.0
2	3.661870226981026E-4	0.0	0.0	0.6598690149019809	0.0
3	3.661870226981026E-4	0.0	0.0	0.9021017304167758	0.0
4	3.661870226981026E-4	0.0	0.0	1.3114988217932544	0.0
5	3.661870226981026E-4	0.0	0.0	2.584731099714557	0.0
6	3.661870226981026E-4	0.0	0.0	3.326992194723611	0.0
7	3.661870226981026E-4	0.0	0.0	4.070351850800759	0.0
8	3.661870226981026E-4	0.0	0.0	5.131195655557162	0.0
9	3.661870226981026E-4	0.0	0.0	5.610900655291677	0.0
10	3.661870226981026E-4	0.0	0.0	6.651421080288336	0.0
11	3.661870226981026E-4	0.0	0.0	7.835853005205348	0.0
12	3.661870226981026E-4	0.0	0.0	8.91042882331293	0.0
13	5.492805340471539E-4	0.0	0.0	9.300784189509107	0.0
14	5.492805340471539E-4	0.0	0.0	9.469413313461585	0.0
15	5.492805340471539E-4	0.0	0.0	9.785066527027348	0.0
16	5.492805340471539E-4	0.0	0.0	10.35027619656187	0.0
17	5.492805340471539E-4	0.0	0.0	10.990004925215455	0.0
18	5.492805340471539E-4	0.0	0.0	11.732632207247207	0.0
19	5.492805340471539E-4	0.0	0.0	12.194027855846816	0.0
20	5.492805340471539E-4	0.0	0.0	12.661648683832293	0.0
21	5.492805340471539E-4	0.0	0.0	13.217154397265315	0.0
22	5.492805340471539E-4	0.0	0.0	13.805250755718468	0.0
23	5.492805340471539E-4	0.0	0.0	14.365516900446565	0.0
24	5.492805340471539E-4	0.0	0.0	14.816476218899279	0.0
25	5.492805340471539E-4	0.0	0.0	15.205183743493315	0.0
26	5.492805340471539E-4	0.0	0.0	15.565511773828247	0.0
27	5.492805340471539E-4	0.0	0.0	15.926389084697227	0.0
28	7.323740453962052E-4	0.0	0.0	16.309420710439444	0.0
29	7.323740453962052E-4	0.0	0.0	16.73785952699622	0.0
30	7.323740453962052E-4	0.0	0.0	17.242282150762858	0.0
31	7.323740453962052E-4	0.0	0.0	17.642524566571886	0.0
32	9.154675567452566E-4	0.0	0.0	18.090005108308965	0.0
33	9.154675567452566E-4	0.0	0.0	18.511852558457182	0.0
34	9.154675567452566E-4	0.0	0.0	18.90989785213002	0.0
35	9.154675567452566E-4	0.0	0.0	19.351153214481233	0.0
36	9.154675567452566E-4	0.0	0.0	19.76439526959604	0.0
37	9.154675567452566E-4	0.0	0.0	20.18496106516481	0.0
38	9.154675567452566E-4	0.0	0.0	20.64489196567363	0.0
39	9.154675567452566E-4	0.0	0.0	21.114709915795295	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACAAT	20	7.0300297E-4	45.000004	20
ATGTACG	20	7.0300297E-4	45.000004	1
TCATCGA	20	7.0300297E-4	45.000004	16
CGGTCTA	25	3.888107E-5	45.000004	31
TCGACAA	20	7.0300297E-4	45.000004	19
GCGTACG	25	3.888107E-5	45.000004	1
AGGGATC	215	0.0	41.860462	6
CGAATAT	120	0.0	41.250004	14
GACGTAG	55	6.002665E-11	40.909092	1
TACGGGT	50	1.0786607E-9	40.500004	4
TAGGGAT	305	0.0	39.836067	5
TACGAAT	125	0.0	39.6	12
ACGGGAT	205	0.0	39.512196	5
GTAGGGA	445	0.0	39.4382	4
ATAGGGT	75	0.0	39.0	4
ACAACGA	185	0.0	38.91892	13
ACGGGTA	70	0.0	38.571426	5
AATATAC	135	0.0	38.333332	16
CGGGTAC	65	9.094947E-12	38.076927	6
ATAGGGC	195	0.0	38.076923	4
>>END_MODULE