SRR3550533_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550533_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	398749
Sequences flagged as poor quality	0
Sequence length	51
%GC	50

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCG	3270	0.820064752513486	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTC	3156	0.7914753391231075	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGC	2977	0.7465849444136536	No Hit
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	1127	0.28263393763996897	TruSeq Adapter, Index 14 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	951	0.2384958959144725	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCT	683	0.17128569601428467	No Hit
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	670	0.1680254997504696	TruSeq Adapter, Index 14 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT	530	0.13291569383246102	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGGCGTAT	485	0.12163039907310114	No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA	471	0.11811941848130025	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTC	446	0.11184981028165587	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTA	443	0.11109745729769856	No Hit
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT	440	0.11034510431374124	Illumina PCR Primer Index 8 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGGTAT	35	1.2092642E-7	45.000004	6
GCCGATA	30	2.1619217E-6	45.000004	9
CGAATAT	30	2.1619217E-6	45.000004	14
ATCGAGG	30	2.1619217E-6	45.000004	2
AACCGTA	20	7.027738E-4	45.0	16
AGCCCGA	20	7.027738E-4	45.0	13
TAAGTAG	20	7.027738E-4	45.0	1
GTCGTTG	20	7.027738E-4	45.0	1
GTCCGCA	20	7.027738E-4	45.0	24
CGATAGA	20	7.027738E-4	45.0	11
TGCGTAG	20	7.027738E-4	45.0	1
CGGGAAT	70	0.0	41.785717	6
AGTACGG	50	1.0786607E-9	40.5	2
TACGGCT	410	0.0	40.060974	7
CGTACCG	45	1.9228537E-8	40.0	45
TAGGGTA	85	0.0	39.705883	5
CGTTAGG	40	3.450641E-7	39.375	2
CGAATGG	40	3.450641E-7	39.375	2
TAGCTAG	40	3.450641E-7	39.375	1
GTACGGG	160	0.0	39.375	3