##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550533_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	398749
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.137570250959875	33.0	31.0	34.0	30.0	34.0
2	32.16648568397664	33.0	31.0	34.0	30.0	34.0
3	32.17937850627838	34.0	31.0	34.0	30.0	34.0
4	35.825709907736446	37.0	35.0	37.0	35.0	37.0
5	35.89009878394679	37.0	35.0	37.0	35.0	37.0
6	35.8331230924717	37.0	35.0	37.0	35.0	37.0
7	36.083383281211994	37.0	35.0	37.0	35.0	37.0
8	35.87607743216911	37.0	35.0	37.0	35.0	37.0
9	37.70842560106734	39.0	38.0	39.0	35.0	39.0
10	37.38982919079421	39.0	37.0	39.0	34.0	39.0
11	37.32903906969046	39.0	37.0	39.0	34.0	39.0
12	37.16063990129129	39.0	37.0	39.0	34.0	39.0
13	37.09586230937256	39.0	37.0	39.0	33.0	39.0
14	38.22220243812524	40.0	37.0	41.0	33.0	41.0
15	38.28842454777316	40.0	37.0	41.0	34.0	41.0
16	38.141690637468685	40.0	37.0	41.0	34.0	41.0
17	38.15495712841913	40.0	37.0	41.0	34.0	41.0
18	38.08080772616358	40.0	37.0	41.0	34.0	41.0
19	37.94882244218794	40.0	36.0	41.0	34.0	41.0
20	38.04425089467309	40.0	36.0	41.0	34.0	41.0
21	38.003633864912516	40.0	36.0	41.0	34.0	41.0
22	38.09695071335602	40.0	36.0	41.0	34.0	41.0
23	38.122763944235594	40.0	36.0	41.0	34.0	41.0
24	38.054580701142825	40.0	36.0	41.0	34.0	41.0
25	37.81587163854957	40.0	36.0	41.0	33.0	41.0
26	37.87374513791884	40.0	36.0	41.0	34.0	41.0
27	37.86023789401353	40.0	36.0	41.0	34.0	41.0
28	37.75567587630314	40.0	36.0	41.0	33.0	41.0
29	37.738484610619714	40.0	36.0	41.0	33.0	41.0
30	37.582250989971136	40.0	35.0	41.0	33.0	41.0
31	37.55845406508856	40.0	35.0	41.0	33.0	41.0
32	37.48599244136035	40.0	35.0	41.0	33.0	41.0
33	37.392492520357415	40.0	35.0	41.0	33.0	41.0
34	37.28155807287291	40.0	35.0	41.0	33.0	41.0
35	37.13257964283296	40.0	35.0	41.0	32.0	41.0
36	37.04886031062147	39.0	35.0	41.0	32.0	41.0
37	36.98643507569925	39.0	35.0	41.0	32.0	41.0
38	36.86113570190771	39.0	35.0	41.0	32.0	41.0
39	36.80995814409566	39.0	35.0	41.0	32.0	41.0
40	36.67138475582384	39.0	35.0	41.0	31.0	41.0
41	36.50705581706788	39.0	35.0	41.0	31.0	41.0
42	36.4679585403349	39.0	35.0	41.0	31.0	41.0
43	36.40715588001475	39.0	35.0	41.0	31.0	41.0
44	36.33306165031135	39.0	35.0	41.0	31.0	41.0
45	36.269989391822925	38.0	35.0	41.0	31.0	41.0
46	36.08955006783716	38.0	35.0	40.0	31.0	41.0
47	35.890326997685264	38.0	35.0	40.0	30.0	41.0
48	35.76989785554321	37.0	35.0	40.0	30.0	41.0
49	35.66626123200309	37.0	35.0	40.0	30.0	41.0
50	35.51523138616022	37.0	35.0	40.0	30.0	41.0
51	34.320078545651526	35.0	33.0	39.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	7.0
10	13.0
11	8.0
12	10.0
13	15.0
14	12.0
15	26.0
16	33.0
17	50.0
18	101.0
19	213.0
20	418.0
21	655.0
22	953.0
23	1281.0
24	1732.0
25	2346.0
26	2927.0
27	3457.0
28	3958.0
29	4668.0
30	5738.0
31	7213.0
32	9546.0
33	13430.0
34	26914.0
35	35971.0
36	30161.0
37	45869.0
38	77307.0
39	123648.0
40	63.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.79070543123619	20.254094681115188	24.271910399775297	11.683289487873322
2	31.101018435155954	27.865148251155492	24.217490200602384	16.816343113086177
3	31.631678073173852	26.977873298741816	25.211348492410014	16.17910013567432
4	28.453989853266087	24.646080617130075	28.585400841130635	18.3145286884732
5	28.3920461242536	29.056624593415908	23.368585250370533	19.182744031959956
6	26.684706419326442	36.12046675979125	22.649085013379345	14.545741807502965
7	78.4257766163677	6.066222109647923	11.064604550732415	4.44339672325197
8	79.65712766677785	7.924784764350506	8.10083536259652	4.317252206275126
9	73.70902497561123	7.499705328414617	9.540588189562857	9.2506815064113
10	44.68575469781742	23.921313909251182	16.31226661383477	15.080664779096626
11	34.904915122044194	23.532347416545246	21.524568086691126	20.038169374719438
12	29.925341505558634	21.379113176459377	26.83342152582201	21.86212379215998
13	26.42489385553318	23.291845246006886	29.74878933865665	20.534471559803286
14	21.960180464402416	27.665022357422842	28.470541618913153	21.90425555926159
15	21.255476502762388	24.22551529909793	33.876699377302515	20.64230882083717
16	24.41836844731899	23.67027879693742	29.000448903947095	22.910903851796494
17	23.478679570356288	23.960185480088978	28.192421799176927	24.368713150377808
18	23.91855528164334	25.02150475612478	28.12746865822861	22.932471304003272
19	26.47103817188256	25.707399893165878	24.71630022896609	23.10526170598547
20	27.025773105387096	26.076303639632954	26.926713295832716	19.97120995914723
21	25.94539422042438	27.115804679133994	26.76044328637815	20.17835781406348
22	24.143759608174566	23.38614015332954	26.914424863761415	25.55567537473448
23	24.65460728428159	26.253357375190912	26.54652425460628	22.54551108592122
24	25.528590667312017	24.471534724851974	26.090347562000154	23.90952704583585
25	24.00457430614246	27.25825017742991	25.332728107155127	23.4044474092725
26	23.198051907340204	25.418496347326265	26.45824817115529	24.92520357417824
27	24.320311775076554	26.429658757764912	26.30100639750821	22.94902306965033
28	22.536984418769702	25.924077552545587	27.869662369059235	23.66927565962548
29	26.13699344700551	24.81385533255256	26.05498697175416	22.99416424868777
30	24.71053218942242	26.600442885123222	26.417621110021592	22.271403815432766
31	28.601952606777697	24.629027282827042	23.28733112810314	23.481688982292116
32	29.122330087348182	26.184642469322807	23.637426049971285	21.055601393357726
33	26.638812887305047	25.232665160288803	22.944759736074573	25.18376221633158
34	26.44495660177204	24.877052983204972	25.39091007124783	23.28708034377516
35	24.571848456046286	25.34225791161859	26.33210365417844	23.753789978156686
36	26.71856230360452	28.730604967034402	23.244447008017573	21.306385721343503
37	26.27241698411783	28.33235945419299	25.199060060338706	20.196163501350473
38	24.899122004067724	27.003453300196362	23.40921231150423	24.688212384231683
39	27.5458998016296	24.248838241600605	24.660876892481234	23.544385064288562
40	24.158054314869755	23.545388201600506	27.99806394498795	24.29849353854179
41	22.77874051094799	26.231539138656146	26.00382696884506	24.9858933815508
42	23.283569363183357	26.124705014934207	27.365084301151853	23.226641320730586
43	24.719560425229904	24.999937303918003	25.368841050385075	24.91166122046701
44	24.553541200103325	24.22476294611397	26.153545212652574	25.06815064113013
45	24.960564164424238	23.5825042821424	25.831788919846822	25.625142633586538
46	25.960190495775535	25.93862304356876	24.94326004579322	23.15792641486248
47	23.120308765664614	24.806833371368956	28.9104173302002	23.162440532766226
48	23.837050375047962	24.87956082648483	26.653609162656206	24.629779635811
49	23.563444673215482	23.294353089286744	29.219383622278677	23.922818615219096
50	22.490338533764348	23.818743119105	28.0218884561466	25.669029890984053
51	22.913662479404337	23.686579778256498	26.733860147611654	26.66589759472751
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	166.0
1	472.0
2	778.0
3	1382.5
4	1987.0
5	1435.0
6	883.0
7	804.5
8	726.0
9	757.0
10	788.0
11	824.5
12	861.0
13	886.0
14	911.0
15	867.5
16	824.0
17	793.0
18	762.0
19	783.0
20	804.0
21	855.0
22	906.0
23	1019.5
24	1133.0
25	1613.0
26	2395.0
27	2697.0
28	3170.0
29	3643.0
30	4099.0
31	4555.0
32	5514.0
33	6473.0
34	7145.5
35	7818.0
36	8677.0
37	9536.0
38	9795.5
39	10055.0
40	12075.5
41	14096.0
42	15522.5
43	16949.0
44	19024.5
45	21100.0
46	22662.5
47	24225.0
48	31828.0
49	39431.0
50	36766.0
51	34101.0
52	34381.5
53	34662.0
54	31106.5
55	27551.0
56	26285.0
57	25019.0
58	23780.0
59	22541.0
60	22019.0
61	21497.0
62	19413.0
63	17329.0
64	15408.5
65	13488.0
66	11500.0
67	9512.0
68	8043.0
69	6574.0
70	5626.5
71	4679.0
72	3831.0
73	2983.0
74	2595.5
75	1778.0
76	1348.0
77	939.5
78	531.0
79	397.5
80	264.0
81	220.5
82	177.0
83	108.5
84	40.0
85	34.0
86	28.0
87	15.5
88	3.0
89	3.0
90	3.0
91	3.5
92	4.0
93	3.0
94	2.0
95	3.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	398749.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.77077582946892
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.83585625039612	22.104450291988933
2	8.251741947866966	5.078250033608792
3	3.024061538329284	2.79158159071348
4	1.8824127083810718	2.3169319787254965
5	1.3937546978072637	2.1443456683748257
6	1.2047997580677792	2.2243573964941215
7	1.1629162926146555	2.5048685583937855
8	0.9700096584775904	2.387835980274691
9	0.9430324870169713	2.611605713211521
>10	9.198213816708703	48.08924241098661
>50	0.08743859106560095	1.8041794220991791
>100	0.03922478851540978	2.581925338831271
>500	0.0032687323762841483	0.7126194400319266
>1k	0.0032687323762841483	2.6478061762654153
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCG	3270	0.820064752513486	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTC	3156	0.7914753391231075	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGC	2977	0.7465849444136536	No Hit
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	1127	0.28263393763996897	TruSeq Adapter, Index 14 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	951	0.2384958959144725	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCT	683	0.17128569601428467	No Hit
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	670	0.1680254997504696	TruSeq Adapter, Index 14 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT	530	0.13291569383246102	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGGCGTAT	485	0.12163039907310114	No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA	471	0.11811941848130025	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTC	446	0.11184981028165587	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTA	443	0.11109745729769856	No Hit
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT	440	0.11034510431374124	Illumina PCR Primer Index 8 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11485922221748518	0.0
2	0.0	0.0	0.0	0.6939202355366408	0.0
3	0.0	0.0	0.0	0.9574945642496909	0.0
4	0.0	0.0	0.0	1.3725426270661494	0.0
5	0.0	0.0	0.0	3.003643896285633	0.0
6	0.0	0.0	0.0	3.6423915796654036	0.0
7	0.0	0.0	0.0	4.360387110688679	0.0
8	0.0	0.0	0.0	5.24841441608631	0.0
9	0.0	0.0	0.0	5.586973258867107	0.0
10	0.0	0.0	0.0	7.085660402910102	0.0
11	0.0	0.0	0.0	8.115882422275668	0.0
12	0.0	0.0	0.0	9.647673097612785	0.0
13	2.507843279857755E-4	0.0	0.0	10.007799392600358	0.0
14	2.507843279857755E-4	0.0	0.0	10.159273126703766	0.0
15	2.507843279857755E-4	0.0	0.0	10.53544961868243	0.0
16	2.507843279857755E-4	0.0	0.0	11.032754941078222	0.0
17	2.507843279857755E-4	0.0	0.0	11.591000855174558	0.0
18	5.01568655971551E-4	0.0	0.0	12.189372261748618	0.0
19	5.01568655971551E-4	0.0	0.0	12.684671309520525	0.0
20	5.01568655971551E-4	0.0	0.0	13.080659763410065	0.0
21	5.01568655971551E-4	0.0	0.0	13.515269003809413	0.0
22	5.01568655971551E-4	0.0	0.0	14.008310992629449	0.0
23	5.01568655971551E-4	0.0	0.0	14.436399840501167	0.0
24	5.01568655971551E-4	0.0	0.0	14.814081038447746	0.0
25	5.01568655971551E-4	0.0	0.0	15.1551477245084	0.0
26	5.01568655971551E-4	0.0	0.0	15.467374212850691	0.0
27	5.01568655971551E-4	0.0	0.0	15.79038442729637	0.0
28	5.01568655971551E-4	0.0	0.0	16.12141974023759	0.0
29	5.01568655971551E-4	0.0	0.0	16.457219955410547	0.0
30	5.01568655971551E-4	0.0	0.0	16.83565350634108	0.0
31	5.01568655971551E-4	0.0	0.0	17.176970976729724	0.0
32	5.01568655971551E-4	0.0	0.0	17.499228838191442	0.0
33	5.01568655971551E-4	0.0	0.0	17.840044739924114	0.0
34	7.523529839573265E-4	0.0	0.0	18.185374759560524	0.0
35	7.523529839573265E-4	0.0	0.0	18.534968112772695	0.0
36	7.523529839573265E-4	0.0	0.0	18.87427930853745	0.0
37	7.523529839573265E-4	0.0	0.0	19.226129720701493	0.0
38	7.523529839573265E-4	0.0	0.0	19.57547228958568	0.0
39	7.523529839573265E-4	0.0	0.0	19.939611133821025	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGGTAT	35	1.2092642E-7	45.000004	6
GCCGATA	30	2.1619217E-6	45.000004	9
CGAATAT	30	2.1619217E-6	45.000004	14
ATCGAGG	30	2.1619217E-6	45.000004	2
AACCGTA	20	7.027738E-4	45.0	16
AGCCCGA	20	7.027738E-4	45.0	13
TAAGTAG	20	7.027738E-4	45.0	1
GTCGTTG	20	7.027738E-4	45.0	1
GTCCGCA	20	7.027738E-4	45.0	24
CGATAGA	20	7.027738E-4	45.0	11
TGCGTAG	20	7.027738E-4	45.0	1
CGGGAAT	70	0.0	41.785717	6
AGTACGG	50	1.0786607E-9	40.5	2
TACGGCT	410	0.0	40.060974	7
CGTACCG	45	1.9228537E-8	40.0	45
TAGGGTA	85	0.0	39.705883	5
CGTTAGG	40	3.450641E-7	39.375	2
CGAATGG	40	3.450641E-7	39.375	2
TAGCTAG	40	3.450641E-7	39.375	1
GTACGGG	160	0.0	39.375	3
>>END_MODULE