##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550532_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	757645
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.205389067439235	33.0	31.0	34.0	30.0	34.0
2	32.229292082703644	34.0	31.0	34.0	30.0	34.0
3	32.21381781705152	34.0	31.0	34.0	30.0	34.0
4	35.86776260649776	37.0	35.0	37.0	35.0	37.0
5	35.93606108401692	37.0	35.0	37.0	35.0	37.0
6	35.88138112176547	37.0	35.0	37.0	35.0	37.0
7	36.152135894779214	37.0	35.0	37.0	35.0	37.0
8	35.97084782450884	37.0	35.0	37.0	35.0	37.0
9	37.84836037986128	39.0	38.0	39.0	35.0	39.0
10	37.479463337050994	39.0	37.0	39.0	35.0	39.0
11	37.43213774261032	39.0	37.0	39.0	35.0	39.0
12	37.42760791663642	39.0	37.0	39.0	35.0	39.0
13	37.426289357152754	39.0	37.0	39.0	35.0	39.0
14	38.64674220776221	40.0	38.0	41.0	35.0	41.0
15	38.71075239723089	40.0	38.0	41.0	35.0	41.0
16	38.508639270370686	40.0	38.0	41.0	34.0	41.0
17	38.506677929637235	40.0	38.0	41.0	34.0	41.0
18	38.40006995360624	40.0	38.0	41.0	34.0	41.0
19	38.23905655023131	40.0	37.0	41.0	34.0	41.0
20	38.34293237598084	40.0	37.0	41.0	34.0	41.0
21	38.3212045219067	40.0	37.0	41.0	34.0	41.0
22	38.41453715130437	40.0	37.0	41.0	34.0	41.0
23	38.41580423549288	40.0	37.0	41.0	34.0	41.0
24	38.364478086702874	40.0	37.0	41.0	34.0	41.0
25	38.15535112090755	40.0	37.0	41.0	34.0	41.0
26	38.218425515907846	40.0	37.0	41.0	34.0	41.0
27	38.23690250711085	40.0	37.0	41.0	34.0	41.0
28	38.149985811296844	40.0	37.0	41.0	34.0	41.0
29	38.153499330161225	40.0	37.0	41.0	34.0	41.0
30	37.97756205082855	40.0	37.0	41.0	34.0	41.0
31	37.92784615486145	40.0	37.0	41.0	34.0	41.0
32	37.78996627708227	40.0	37.0	41.0	33.0	41.0
33	37.67018722488765	40.0	36.0	41.0	33.0	41.0
34	37.54038632868956	40.0	36.0	41.0	33.0	41.0
35	37.43407928515334	40.0	36.0	41.0	33.0	41.0
36	37.31011753525728	40.0	36.0	41.0	33.0	41.0
37	37.28633330913554	40.0	36.0	41.0	33.0	41.0
38	37.129122478205495	40.0	36.0	41.0	32.0	41.0
39	37.074292049706656	40.0	35.0	41.0	32.0	41.0
40	36.96688554666103	40.0	35.0	41.0	32.0	41.0
41	36.84249615585136	40.0	35.0	41.0	31.0	41.0
42	36.83877541592698	40.0	35.0	41.0	31.0	41.0
43	36.77612866184031	40.0	35.0	41.0	31.0	41.0
44	36.685298523714934	39.0	35.0	41.0	31.0	41.0
45	36.66220591437943	39.0	35.0	41.0	31.0	41.0
46	36.54377445901444	39.0	35.0	41.0	31.0	41.0
47	36.448782741257446	39.0	35.0	41.0	31.0	41.0
48	36.35996409928133	39.0	35.0	41.0	31.0	41.0
49	36.33547769733846	39.0	35.0	41.0	31.0	41.0
50	36.2127619135611	39.0	35.0	41.0	30.0	41.0
51	35.11030099848874	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	6.0
9	12.0
10	25.0
11	25.0
12	23.0
13	21.0
14	15.0
15	33.0
16	40.0
17	79.0
18	144.0
19	328.0
20	610.0
21	1128.0
22	1696.0
23	2213.0
24	3152.0
25	4556.0
26	6602.0
27	7798.0
28	8159.0
29	8457.0
30	9441.0
31	11719.0
32	15217.0
33	20824.0
34	39014.0
35	52571.0
36	49911.0
37	75776.0
38	146916.0
39	290952.0
40	178.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.3981614080473	19.490262589999276	24.05176566861789	13.059810333335534
2	32.83199915527721	26.08371994799675	24.23192920167097	16.85235169505507
3	30.539368701700663	26.723333487319255	27.624415128457258	15.112882682522818
4	26.95286050854952	24.638452045483042	30.373855829577177	18.034831616390264
5	26.234186195381742	28.979799246348886	25.94552857868791	18.840485979581466
6	25.1347266859809	35.20619815348877	25.67086168324215	13.988213477288175
7	79.66224287100158	5.14726553992965	11.309650297962765	3.880841291105993
8	81.08441288466234	6.0882075378310425	9.06968303097097	3.7576965465356467
9	75.0266945601172	6.938737799365138	10.560090807700176	7.474476832817481
10	36.36003669264629	33.56004461192247	16.94553517808472	13.134383517346516
11	27.443987619531573	23.877277616825822	29.98528334510226	18.693451418540345
12	24.846860996904883	22.382778214071234	31.95335546331065	20.817005325713232
13	24.076315424770176	22.958905556032178	33.77927657412113	19.18550244507652
14	19.99089283239512	28.056543631912046	30.88082149291555	21.071742042777288
15	19.420704947567792	25.82225184618126	34.23687874928232	20.520164456968633
16	23.395653637257556	25.702538787954783	30.436418111384622	20.465389463403046
17	22.1999749222921	25.49756152287681	30.384678840353992	21.917784714477094
18	23.76389997954187	24.96050261006144	29.62099664090702	21.65460076948967
19	23.116895115786416	26.90574081528948	28.05825947508398	21.91910459384012
20	25.443842432801645	26.26282757755941	29.1818727768282	19.11145721281075
21	24.056121270515877	27.828864441790017	29.320724085818554	18.794290201875548
22	23.24096377591088	23.327151898316494	29.771726864164616	23.66015746160801
23	22.337902315728343	26.928970692078746	29.906222571256986	20.826904420935925
24	22.98358730012077	24.929485445030323	30.168614588626603	21.918312666222306
25	22.42950194352236	27.993585386295695	28.298873482963653	21.27803918721829
26	21.224188109206818	26.512020801298764	29.318084327092503	22.945706762401915
27	22.690574081528947	25.617670544912198	29.061763754792814	22.629991618766045
28	20.075893063374007	27.159685604735728	30.73616271472787	22.02825861716239
29	21.758211299487225	25.106613255548442	31.406793419081495	21.728382025882834
30	22.5271730163863	24.600307531891584	32.51324828910638	20.359271162615737
31	24.796573593173584	24.851480574675474	28.693649400444798	21.65829643170614
32	24.157224029723682	25.71969721967412	30.396953718430137	19.72612503217206
33	22.720007391324433	26.29806835655221	28.152366873667745	22.829557378455608
34	21.638234265388142	26.677665661358553	29.821486316150704	21.8626137571026
35	20.824000686337268	26.922767259072522	30.264569818318606	21.988662236271605
36	23.152531858588123	28.466630150004292	27.898950035966713	20.48188795544087
37	22.304905331652687	26.794606972922676	29.468814550350096	21.43167314507454
38	22.24762256729735	28.367771185713625	27.152030304430173	22.23257594255885
39	23.351833642405083	25.429587735680958	27.835595826541454	23.382982795372502
40	22.65889697681632	26.40682641606557	29.51646219535534	21.417814411762766
41	20.7701496083258	27.015554778293264	28.543051165123508	23.67124444825743
42	22.4164351378284	27.06056266457246	29.035234179595985	21.487768018003152
43	23.684179266015086	25.161124273241427	28.52510080578635	22.62959565495714
44	22.333018762085146	25.23807324010585	28.691009641718747	23.737898356090252
45	22.256993710774836	24.081858918094888	28.856390525905933	24.804756845224347
46	23.33163948815078	26.05428663820127	27.789796012644448	22.8242778610035
47	20.825188577763992	26.113021269855935	31.034851414580704	22.026938737799366
48	22.07353047931419	25.864751961670702	28.829728962772805	23.231988596242303
49	22.019547413366418	24.7369150459648	30.571045806413295	22.67249173425549
50	20.58153884734935	24.676728547010804	30.720192174435258	24.02154043120459
51	20.70587148334642	24.18071788238555	29.062819658283235	26.050590975984793
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	319.0
1	660.5
2	1002.0
3	5014.0
4	9026.0
5	6056.0
6	3086.0
7	2933.0
8	2780.0
9	2761.5
10	2743.0
11	2664.5
12	2586.0
13	2473.5
14	2361.0
15	2263.0
16	2165.0
17	2211.5
18	2258.0
19	2137.0
20	2016.0
21	2213.0
22	2410.0
23	2992.5
24	3575.0
25	4231.0
26	5740.5
27	6594.0
28	8235.5
29	9877.0
30	11670.5
31	13464.0
32	15409.0
33	17354.0
34	19753.5
35	22153.0
36	23280.0
37	24407.0
38	27822.5
39	31238.0
40	33738.0
41	36238.0
42	39066.0
43	41894.0
44	58159.5
45	74425.0
46	69832.0
47	65239.0
48	63787.0
49	62335.0
50	57949.0
51	53563.0
52	50036.5
53	46510.0
54	42578.5
55	38647.0
56	37913.5
57	37180.0
58	35069.0
59	32958.0
60	31696.0
61	30434.0
62	27507.5
63	24581.0
64	20509.0
65	16437.0
66	13756.0
67	11075.0
68	8768.5
69	6462.0
70	5579.0
71	4696.0
72	4038.0
73	3380.0
74	2966.5
75	1933.5
76	1314.0
77	1048.5
78	783.0
79	584.0
80	385.0
81	273.5
82	162.0
83	107.0
84	52.0
85	37.5
86	23.0
87	15.5
88	8.0
89	4.5
90	1.0
91	3.0
92	5.0
93	4.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	757645.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.1057888162587
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.42335838559013	20.78833185718946
2	8.20868803337019	4.778406807156454
3	3.210489130020123	2.8033145584577936
4	1.9660711391044936	2.288962054900663
5	1.4731208131001343	2.14381716434639
6	1.1981472839077023	2.092381308971492
7	1.0408309189131533	2.1205943443523103
8	0.9521719191727461	2.2170971836970956
9	0.9131842966249782	2.392105435910132
>10	9.492217896313273	50.85199395977623
>50	0.07795562339926954	1.5394428592206983
>100	0.03738222874662353	1.9495805828417974
>500	0.0031911658686142033	0.6710017700558748
>1k	0.002279404191867288	1.6980046769636208
>5k	9.117616767469153E-4	1.6649654361599997
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTC	7421	0.9794824753017575	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5127	0.6767021494235427	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCG	4330	0.571507764190353	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGC	3608	0.47621247417985996	No Hit
GAACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCT	2174	0.2869417735219001	No Hit
GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC	1666	0.21989190188016816	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTT	1019	0.13449570709237177	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCT	988	0.13040408106699047	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTC	865	0.11416956490176797	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.639758726052439E-4	0.0	0.0	0.09239155541183536	0.0
2	3.959638089078658E-4	0.0	0.0	0.45139874215496706	0.0
3	3.959638089078658E-4	0.0	0.0	0.6604676332583201	0.0
4	3.959638089078658E-4	0.0	0.0	1.213893050175214	0.0
5	3.959638089078658E-4	0.0	0.0	2.8860482151931315	0.0
6	3.959638089078658E-4	0.0	0.0	3.6959261923460196	0.0
7	3.959638089078658E-4	0.0	0.0	4.3645770776551025	0.0
8	3.959638089078658E-4	0.0	0.0	5.292584257798837	0.0
9	3.959638089078658E-4	0.0	0.0	5.704914570808228	0.0
10	3.959638089078658E-4	0.0	0.0	6.963287555517426	0.0
11	3.959638089078658E-4	0.0	0.0	7.7597027631674464	0.0
12	3.959638089078658E-4	0.0	0.0	8.885823835701418	0.0
13	3.959638089078658E-4	0.0	0.0	9.200879039655776	0.0
14	3.959638089078658E-4	0.0	0.0	9.35517293719354	0.0
15	3.959638089078658E-4	0.0	0.0	9.620732665034415	0.0
16	3.959638089078658E-4	0.0	0.0	9.993730573025626	0.0
17	5.279517452104877E-4	0.0	0.0	10.458064132938249	0.0
18	5.279517452104877E-4	0.0	0.0	10.942591847105174	0.0
19	5.279517452104877E-4	0.0	0.0	11.343439209656237	0.0
20	5.279517452104877E-4	0.0	0.0	11.668789472642201	0.0
21	5.279517452104877E-4	0.0	0.0	12.037299790799121	0.0
22	5.279517452104877E-4	0.0	0.0	12.521695517029743	0.0
23	6.599396815131097E-4	0.0	0.0	12.952636129057804	0.0
24	6.599396815131097E-4	0.0	0.0	13.281286090451333	0.0
25	6.599396815131097E-4	0.0	0.0	13.568887803654746	0.0
26	6.599396815131097E-4	0.0	0.0	13.844082650845714	0.0
27	7.919276178157316E-4	0.0	0.0	14.127064786278535	0.0
28	7.919276178157316E-4	0.0	0.0	14.424301618832038	0.0
29	7.919276178157316E-4	0.0	0.0	14.738168931359674	0.0
30	7.919276178157316E-4	0.0	0.0	15.149971292623855	0.0
31	7.919276178157316E-4	0.0	0.0	15.478357278144777	0.0
32	7.919276178157316E-4	0.0	0.0	15.826805429983699	0.0
33	7.919276178157316E-4	0.0	0.0	16.161262860574542	0.0
34	7.919276178157316E-4	0.0	0.0	16.488064990859836	0.0
35	7.919276178157316E-4	0.0	0.0	16.849447960456413	0.0
36	7.919276178157316E-4	0.0	0.0	17.19512436563298	0.0
37	7.919276178157316E-4	0.0	0.0	17.53129763939576	0.0
38	7.919276178157316E-4	0.0	0.0	17.866019045859208	0.0
39	7.919276178157316E-4	0.0	0.0	18.22858990688251	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGTCG	20	7.0317637E-4	45.0	1
GCGCCTA	20	7.0317637E-4	45.0	19
CGAATAA	25	3.889543E-5	45.0	41
CCGCACA	20	7.0317637E-4	45.0	43
CGTCCAA	30	2.1645192E-6	44.999996	29
CGTAAGG	120	0.0	43.124996	2
CGTTTTT	2845	0.0	41.441124	1
TTAACGG	50	1.0804797E-9	40.5	2
CGTCATA	50	1.0804797E-9	40.5	38
GCGTAAG	50	1.0804797E-9	40.5	1
AACACGT	335	0.0	40.298504	41
CGACGGT	45	1.9268555E-8	40.0	28
CGGACGG	85	0.0	39.705883	2
GCTACGA	120	0.0	39.374996	10
ACACGAC	340	0.0	39.04412	26
CACGACC	340	0.0	39.04412	27
CGAACCC	35	6.245469E-6	38.57143	34
TCAAGCG	380	0.0	38.486843	17
GACACGA	350	0.0	37.92857	25
CGAATAT	125	0.0	37.800003	14
>>END_MODULE