FastQCFastQC Report
Sat 18 Jun 2016
SRR3550531_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550531_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences570889
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90751.5896260043546118No Hit
CGCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCTG17710.3102179232740515No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTT17000.29778117987910085No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTC11650.2040676909171485No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCT10710.18760214332383351No Hit
CGTCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCT8090.14170880854246623No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTC7060.12366677234979129No Hit
CCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCTGC6540.11455817155348938No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAG253.8883307E-545.01
CGCATCG207.0303015E-445.021
ACGATAG207.0303015E-445.01
AATTGCG207.0303015E-445.01
GCGATCG207.0303015E-445.09
CCAGTCG207.0303015E-445.028
TCAACGG207.0303015E-445.02
CGGACTT207.0303015E-445.015
TTATACG302.1635751E-644.9999961
CGTTTTT60550.043.99671
GCGCGAC556.002665E-1140.9090929
TACGGGT451.9254003E-840.04
TGTTGCG403.4541517E-739.3751
AACACGT403.4541517E-739.37541
ACACGGG1150.039.1304363
TTTACGG356.2427644E-638.5714262
GCGAAAG700.038.5714261
TTCGAAG356.2427644E-638.5714261
AAGGGCG1650.038.1818165
GTTTTTT71400.037.9726872