##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550531_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	570889
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.24043728290438	33.0	31.0	34.0	30.0	34.0
2	32.40426247484187	34.0	31.0	34.0	30.0	34.0
3	32.37708381138891	34.0	31.0	34.0	30.0	34.0
4	35.943025702019135	37.0	35.0	37.0	35.0	37.0
5	35.93039102172226	37.0	35.0	37.0	35.0	37.0
6	35.95606676604384	37.0	35.0	37.0	35.0	37.0
7	36.12598421059085	37.0	35.0	37.0	35.0	37.0
8	36.14220802993226	37.0	36.0	37.0	35.0	37.0
9	37.78450276673749	39.0	38.0	39.0	35.0	39.0
10	37.48939285920731	39.0	37.0	39.0	35.0	39.0
11	37.44810812609807	39.0	37.0	39.0	35.0	39.0
12	37.41718267474062	39.0	37.0	39.0	35.0	39.0
13	37.40575663570326	39.0	37.0	39.0	35.0	39.0
14	38.68561489186164	40.0	38.0	41.0	35.0	41.0
15	38.74265224938648	40.0	38.0	41.0	35.0	41.0
16	38.742231852426656	40.0	38.0	41.0	35.0	41.0
17	38.74492414462356	40.0	38.0	41.0	35.0	41.0
18	38.53430001278707	40.0	38.0	41.0	35.0	41.0
19	38.330990788051615	40.0	37.0	41.0	34.0	41.0
20	38.05825125374635	40.0	36.0	41.0	34.0	41.0
21	38.016591666681265	40.0	35.0	41.0	34.0	41.0
22	37.99343480081067	40.0	35.0	41.0	34.0	41.0
23	37.963707480788734	40.0	35.0	41.0	34.0	41.0
24	37.92577891674213	40.0	35.0	41.0	34.0	41.0
25	37.90182154499386	40.0	35.0	41.0	34.0	41.0
26	37.76963122428353	40.0	35.0	41.0	34.0	41.0
27	37.70039709996164	40.0	35.0	41.0	33.0	41.0
28	37.591586105179815	40.0	35.0	41.0	33.0	41.0
29	37.50194696342021	40.0	35.0	41.0	33.0	41.0
30	37.39561105573938	40.0	35.0	41.0	33.0	41.0
31	37.160307870706916	40.0	35.0	41.0	33.0	41.0
32	36.763199150798144	39.0	35.0	41.0	31.0	41.0
33	36.32337634811671	39.0	35.0	41.0	30.0	41.0
34	35.9093589121528	39.0	35.0	41.0	26.0	41.0
35	35.698999280070204	39.0	35.0	41.0	24.0	41.0
36	35.50637164142241	39.0	35.0	41.0	23.0	41.0
37	35.42226071968456	39.0	35.0	41.0	23.0	41.0
38	35.30535007681003	39.0	35.0	41.0	23.0	41.0
39	35.21440770447495	39.0	35.0	41.0	22.0	41.0
40	35.1103349337612	39.0	35.0	41.0	22.0	41.0
41	34.955264508512165	39.0	35.0	41.0	21.0	41.0
42	34.85794961892767	39.0	35.0	41.0	20.0	41.0
43	34.78837917703792	39.0	34.0	41.0	20.0	41.0
44	34.6192131920566	39.0	34.0	41.0	18.0	41.0
45	34.574856057832605	38.0	34.0	41.0	20.0	41.0
46	34.49085023533471	38.0	34.0	40.0	20.0	41.0
47	34.401752354660886	38.0	34.0	40.0	20.0	41.0
48	34.254427743396704	38.0	34.0	40.0	18.0	41.0
49	34.156855360674314	38.0	34.0	40.0	18.0	41.0
50	34.070710768643295	38.0	33.0	40.0	18.0	41.0
51	32.8906267242844	36.0	31.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	2.0
12	2.0
13	12.0
14	6.0
15	22.0
16	31.0
17	73.0
18	158.0
19	349.0
20	601.0
21	986.0
22	1592.0
23	2559.0
24	4543.0
25	7989.0
26	12085.0
27	13248.0
28	12271.0
29	10966.0
30	11014.0
31	11905.0
32	13661.0
33	17256.0
34	27728.0
35	36794.0
36	38702.0
37	55212.0
38	99637.0
39	191321.0
40	159.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.72060418049743	20.584561972642668	23.658014079794846	20.03681976706505
2	38.95748560578326	22.77938443375157	22.726834813772907	15.536295146692265
3	28.10160994519075	22.34006961072993	34.47938215660137	15.07893828747795
4	25.096997840210616	23.688142528582613	34.455734827610975	16.759124803595796
5	23.749275253157794	27.038706298422284	32.4406320668291	16.771386381590816
6	23.98224523506321	31.33393707007842	31.079947240181543	13.603870454676828
7	74.02892681414426	5.043362194752395	16.501631665700337	4.426079325403012
8	76.19256983406582	4.13950873111936	15.925337499934313	3.7425839348805114
9	70.05687620535691	6.716892425672942	17.61585877464796	5.610372594322189
10	34.546295339374204	27.629889523182268	24.800968314330806	13.022846823112724
11	25.124673973399382	23.304880633538218	33.91622539583001	17.65421999723239
12	23.506496008856363	21.343553650534517	36.314765217056205	18.835185123552915
13	21.450054213691278	21.11426214202761	38.47963439477727	18.956049249503845
14	18.528645673677367	24.80482195312924	36.83623261264449	19.830299760548897
15	18.029249118480127	24.826367297320495	39.01949415735809	18.124889426841296
16	20.91825205950719	23.99590813625766	37.4300433184034	17.655796485831747
17	20.553908027655112	24.376367384903194	35.3049366864662	19.7647879009755
18	20.787053174960455	25.066869391422852	34.964765479804306	19.18131195381239
19	21.356691055529183	26.385689687487414	33.822161576068204	18.435457680915203
20	22.645032572006116	25.025355191639704	33.91219659163165	18.417415644722528
21	21.97169677467949	25.10470511780749	34.57344597636318	18.350152131149837
22	21.154725349411184	23.671326650189442	34.47938215660137	20.694565843798006
23	20.39608400231919	25.366577390701167	34.50232882399206	19.735009782987586
24	20.23650832298398	23.778878205745777	35.559276847162934	20.425336624107313
25	21.221113035984228	25.3837435998942	33.24253926770353	20.15260409641804
26	20.328119827146786	26.627417939389268	33.857019490654054	19.1874427428099
27	21.70176689338908	24.98734429985514	33.78520167668321	19.525687130072573
28	19.795091515163193	26.015565197437677	34.52194734878409	19.667395938615037
29	21.76955590316156	23.935476073282196	34.15917980553137	20.13578821802487
30	21.5435925372533	23.746122275959074	34.32401044686445	20.38627473992317
31	21.164359446407268	24.651902558991328	34.14166326553848	20.042074729062918
32	22.237773017171463	25.335748280313688	33.43084207262707	18.995636629887773
33	21.348458281732526	25.972474509055175	33.15618272553859	19.522884483673707
34	21.909162726904878	25.66435857058027	33.25655249969784	19.16992620281701
35	21.86537137692266	25.44452599366952	31.737518151514564	20.95258447789325
36	21.73662480797493	26.609200737796662	31.190651772936594	20.46352268129181
37	22.8687187877153	26.723233413150364	31.173660729143492	19.234387069990838
38	21.43901879349576	26.012061889439103	31.268250045105088	21.280669271960047
39	23.403673919098107	25.114864711003364	30.537109665801932	20.944351704096594
40	22.982751463069004	24.507566269449928	32.1901455449308	20.319536722550268
41	21.542891875653584	25.613910935400753	31.360036714667828	21.483160474277838
42	22.3059123577438	26.30896724231856	30.61470793797043	20.770412461967215
43	22.321151747537613	25.67189068277721	31.112352839168384	20.894604730516793
44	22.206768741384053	26.276911974131572	30.4544315970355	21.061887687448873
45	21.480708158678834	24.83389940951744	30.779188248503647	22.90620418330008
46	21.962763339283118	25.589037448610853	29.9783320400288	22.469867172077233
47	21.74958704756967	25.149021963989497	31.63346990395681	21.467921084484022
48	21.517668058063826	24.108364323012005	31.743999271311935	22.629968347612234
49	21.16593593500663	24.53524240263869	31.769923750501412	22.528897911853267
50	19.664943623016033	24.601104593011954	32.564123673778965	23.16982811019305
51	20.16066170481477	24.148827530395575	31.316245364685603	24.374265400104047
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	184.0
1	433.0
2	682.0
3	8295.0
4	15908.0
5	10873.0
6	5838.0
7	5774.0
8	5710.0
9	5636.5
10	5563.0
11	5488.0
12	5413.0
13	5240.5
14	5068.0
15	4756.5
16	4445.0
17	4193.0
18	3941.0
19	3833.0
20	3725.0
21	3467.5
22	3210.0
23	3446.0
24	3682.0
25	4004.0
26	5084.0
27	5842.0
28	6605.5
29	7369.0
30	8646.5
31	9924.0
32	10876.0
33	11828.0
34	13417.5
35	15007.0
36	17022.5
37	19038.0
38	20625.0
39	22212.0
40	24569.0
41	26926.0
42	28810.0
43	30694.0
44	33038.0
45	35382.0
46	41495.5
47	47609.0
48	48757.0
49	49905.0
50	49020.0
51	48135.0
52	42099.5
53	36064.0
54	32088.0
55	28112.0
56	25821.0
57	23530.0
58	22702.5
59	21875.0
60	21011.5
61	20148.0
62	18013.5
63	15879.0
64	13048.5
65	10218.0
66	8194.5
67	6171.0
68	5226.0
69	4281.0
70	3388.0
71	2495.0
72	2187.5
73	1880.0
74	1576.0
75	966.5
76	661.0
77	499.0
78	337.0
79	275.5
80	214.0
81	154.5
82	95.0
83	75.0
84	55.0
85	39.5
86	24.0
87	15.5
88	7.0
89	4.5
90	2.0
91	1.0
92	0.0
93	1.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	570889.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.04710682036428
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.98205600058357	30.686843046263455
2	8.579877049035577	7.215180135727815
3	4.098730903359052	5.170193283643987
4	2.647167750222936	4.452229806601887
5	2.0304324324626406	4.268690468964437
6	1.7438919170001528	4.399536583036515
7	1.3552045669847876	3.9887701834018414
8	1.1486524378945961	3.8638009404500746
9	0.9245620605123017	3.4987643748375166
>10	4.445401536705021	27.3735249470536
>50	0.023059847530595753	0.6575650040262451
>100	0.01760933807535571	1.4365944259208532
>500	0.0012578098625254078	0.3823749306445368
>1k	0.0016770798167005437	1.0060920835354412
>5k	4.1926995417513593E-4	1.5998397858917344
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9075	1.5896260043546118	No Hit
CGCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCTG	1771	0.3102179232740515	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTT	1700	0.29778117987910085	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTC	1165	0.2040676909171485	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCT	1071	0.18760214332383351	No Hit
CGTCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCT	809	0.14170880854246623	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTC	706	0.12366677234979129	No Hit
CCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCTGC	654	0.11455817155348938	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09546514296124115	0.0
2	0.0	0.0	0.0	0.3858893760433289	0.0
3	0.0	0.0	0.0	0.883709442641214	0.0
4	0.0	0.0	0.0	1.271000141883974	0.0
5	0.0	0.0	0.0	2.2869594614714943	0.0
6	0.0	0.0	0.0	3.467048760792378	0.0
7	0.0	0.0	0.0	4.374931028623778	0.0
8	0.0	0.0	0.0	5.597235189327523	0.0
9	0.0	0.0	0.0	6.294218315644548	0.0
10	0.0	0.0	0.0	7.26603595444999	0.0
11	0.0	0.0	0.0	8.24135690125401	0.0
12	0.0	0.0	0.0	9.065685273319332	0.0
13	0.0	0.0	0.0	9.500971292142605	0.0
14	0.0	0.0	0.0	9.74340020564418	0.0
15	0.0	0.0	0.0	9.991959908143265	0.0
16	0.0	0.0	0.0	10.394665162579766	0.0
17	0.0	0.0	0.0	10.888281259579358	0.0
18	0.0	0.0	0.0	11.416054609565082	0.0
19	0.0	0.0	0.0	11.774618183219506	0.0
20	0.0	0.0	0.0	12.11251223968232	0.0
21	0.0	0.0	0.0	12.52309993711562	0.0
22	0.0	0.0	0.0	13.016365703315355	0.0
23	0.0	0.0	0.0	13.50945630411516	0.0
24	0.0	0.0	0.0	13.906030769554151	0.0
25	0.0	0.0	0.0	14.260040042810424	0.0
26	1.7516539992888284E-4	0.0	0.0	14.582519544079497	0.0
27	3.503307998577657E-4	0.0	0.0	14.932675178537334	0.0
28	3.503307998577657E-4	0.0	0.0	15.287209947993393	0.0
29	3.503307998577657E-4	0.0	0.0	15.673099324036722	0.0
30	3.503307998577657E-4	0.0	0.0	16.098576080463978	0.0
31	3.503307998577657E-4	0.0	0.0	16.514593905295076	0.0
32	3.503307998577657E-4	0.0	0.0	16.91081803993421	0.0
33	3.503307998577657E-4	0.0	0.0	17.26482731319048	0.0
34	3.503307998577657E-4	0.0	0.0	17.622865390645117	0.0
35	3.503307998577657E-4	0.0	0.0	17.984406776098332	0.0
36	3.503307998577657E-4	0.0	0.0	18.3923669925327	0.0
37	5.254961997866486E-4	0.0	0.0	18.79857555496778	0.0
38	5.254961997866486E-4	0.0	0.0	19.205309613602644	0.0
39	5.254961997866486E-4	0.0	0.0	19.631487031629618	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAG	25	3.8883307E-5	45.0	1
CGCATCG	20	7.0303015E-4	45.0	21
ACGATAG	20	7.0303015E-4	45.0	1
AATTGCG	20	7.0303015E-4	45.0	1
GCGATCG	20	7.0303015E-4	45.0	9
CCAGTCG	20	7.0303015E-4	45.0	28
TCAACGG	20	7.0303015E-4	45.0	2
CGGACTT	20	7.0303015E-4	45.0	15
TTATACG	30	2.1635751E-6	44.999996	1
CGTTTTT	6055	0.0	43.9967	1
GCGCGAC	55	6.002665E-11	40.909092	9
TACGGGT	45	1.9254003E-8	40.0	4
TGTTGCG	40	3.4541517E-7	39.375	1
AACACGT	40	3.4541517E-7	39.375	41
ACACGGG	115	0.0	39.130436	3
TTTACGG	35	6.2427644E-6	38.571426	2
GCGAAAG	70	0.0	38.571426	1
TTCGAAG	35	6.2427644E-6	38.571426	1
AAGGGCG	165	0.0	38.181816	5
GTTTTTT	7140	0.0	37.972687	2
>>END_MODULE