FastQCFastQC Report
Sat 18 Jun 2016
SRR3550529_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550529_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences636406
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59480.9346234950644714No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTT8780.13796224422774142No Hit
CGCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCTG7930.12460599051548854No Hit
GCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCTGC6900.10842135366417036No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTC6380.10025046904020389No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCACGC207.030911E-445.022
TCTAGCG406.8066583E-945.01
CTAACGG207.030911E-445.02
CGTAGTA207.030911E-445.038
TTGACCG253.888838E-544.9999961
GTATACG253.888838E-544.9999961
GCGTAAG502.1827873E-1144.9999961
CGTTTTT33400.042.844311
TAGACGG650.041.5384642
TATACGG603.6379788E-1241.2500042
ACGGGCC556.002665E-1140.9090925
GCGTACG501.0804797E-940.4999961
GACACGA1850.040.13513625
CACGACC2050.039.51219627
GCGTTAG750.039.01
ACGTACG356.2438958E-638.5714261
GTAAGCG356.2438958E-638.5714261
GCGAGAC2000.038.24999621
TCAAGCG2000.038.24999617
AGGGATC5300.038.2075466