##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3550529_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 636406 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.29914551402721 33.0 31.0 34.0 30.0 34.0 2 32.42632219055132 34.0 31.0 34.0 31.0 34.0 3 32.47721580249086 34.0 31.0 34.0 30.0 34.0 4 35.98553124891971 37.0 35.0 37.0 35.0 37.0 5 35.95858618554822 37.0 35.0 37.0 35.0 37.0 6 35.978779269837176 37.0 35.0 37.0 35.0 37.0 7 36.15919083101039 37.0 35.0 37.0 35.0 37.0 8 36.16234290688649 37.0 36.0 37.0 35.0 37.0 9 37.75257775696647 39.0 38.0 39.0 35.0 39.0 10 37.50290380668944 39.0 37.0 39.0 35.0 39.0 11 37.434376797201786 39.0 37.0 39.0 35.0 39.0 12 37.37936631647093 39.0 37.0 39.0 35.0 39.0 13 37.362592433132306 39.0 37.0 39.0 34.0 39.0 14 38.61648538825844 40.0 38.0 41.0 35.0 41.0 15 38.6715791491595 40.0 38.0 41.0 35.0 41.0 16 38.67757689273828 40.0 38.0 41.0 35.0 41.0 17 38.67058292976496 40.0 38.0 41.0 35.0 41.0 18 38.558420253737395 40.0 38.0 41.0 34.0 41.0 19 38.476048308784016 40.0 37.0 41.0 34.0 41.0 20 38.31946587555743 40.0 37.0 41.0 34.0 41.0 21 38.28013406536079 40.0 37.0 41.0 34.0 41.0 22 38.255294576103935 40.0 37.0 41.0 34.0 41.0 23 38.20850211971603 40.0 37.0 41.0 34.0 41.0 24 38.19170152386998 40.0 37.0 41.0 34.0 41.0 25 38.15439986423761 40.0 37.0 41.0 34.0 41.0 26 38.03957693673536 40.0 36.0 41.0 34.0 41.0 27 37.99062705254193 40.0 36.0 41.0 34.0 41.0 28 37.89817035037382 40.0 36.0 41.0 34.0 41.0 29 37.8413339911943 40.0 36.0 41.0 33.0 41.0 30 37.79236210846535 40.0 36.0 41.0 33.0 41.0 31 37.64740432994032 40.0 36.0 41.0 33.0 41.0 32 37.434854479687495 40.0 36.0 41.0 33.0 41.0 33 37.24867301691059 40.0 36.0 41.0 32.0 41.0 34 36.98245931056589 40.0 35.0 41.0 31.0 41.0 35 36.91520193084289 40.0 35.0 41.0 31.0 41.0 36 36.769854463974255 40.0 35.0 41.0 31.0 41.0 37 36.73467094904825 40.0 35.0 41.0 31.0 41.0 38 36.65497968278112 40.0 35.0 41.0 30.0 41.0 39 36.54193549400854 39.0 35.0 41.0 30.0 41.0 40 36.485825086501386 39.0 35.0 41.0 30.0 41.0 41 36.35144074694456 39.0 35.0 41.0 30.0 41.0 42 36.29037438364817 39.0 35.0 41.0 30.0 41.0 43 36.21262684512717 39.0 35.0 41.0 29.0 41.0 44 36.05330716555155 39.0 35.0 41.0 28.0 41.0 45 35.9956788590931 39.0 35.0 41.0 28.0 41.0 46 35.90489875959686 39.0 35.0 41.0 28.0 41.0 47 35.838719308114634 39.0 35.0 41.0 28.0 41.0 48 35.72687561085219 39.0 35.0 41.0 28.0 41.0 49 35.64452566443434 39.0 35.0 40.0 27.0 41.0 50 35.554377237172496 38.0 35.0 40.0 27.0 41.0 51 34.40598454445747 37.0 33.0 40.0 24.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 7.0 11 3.0 12 5.0 13 6.0 14 5.0 15 20.0 16 33.0 17 68.0 18 131.0 19 283.0 20 477.0 21 680.0 22 1190.0 23 1791.0 24 2962.0 25 4668.0 26 7060.0 27 8521.0 28 9000.0 29 9363.0 30 10252.0 31 11874.0 32 14759.0 33 19397.0 34 32420.0 35 43294.0 36 43293.0 37 63219.0 38 118420.0 39 233043.0 40 161.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.03027312753179 21.318780778308184 25.02899092717542 14.621955166984598 2 33.988680182147874 24.177804734713373 25.347655427510112 16.48585965562864 3 30.24547222999155 23.533876173386172 30.153864042765154 16.06678755385713 4 26.962976464709637 25.425121699041174 29.932307363538367 17.679594472710818 5 24.664600899425835 29.65716853706596 27.508697278152628 18.169533285355573 6 26.185485366259904 33.153521494140534 25.938473238781533 14.722519900818032 7 80.26354245560225 5.173584158540303 10.469731586440103 4.093141799417353 8 82.14504577266713 4.275101114697222 9.922125184237734 3.6577279283979096 9 75.25353312193789 7.515485397686382 11.99470149558615 5.236279984789584 10 36.434131670663064 33.782365345392726 17.63292615091624 12.150576833027973 11 26.242681558627666 24.261713434505648 31.531758028679807 17.963846978186883 12 25.181095087098488 22.436620647825446 32.8845108311361 19.49777343393997 13 22.874077240000883 23.00198301084528 34.06017542260758 20.06376432654626 14 19.683346794341976 26.022853335763646 32.99937461306147 21.294425256832902 15 19.690574884586255 25.770812971593603 35.204884931945955 19.33372721187418 16 22.489731397881226 25.490803040826137 33.38104920443868 18.638416356853956 17 21.90237049933533 25.518615475026944 31.641122176723663 20.93789184891406 18 22.72354440404396 25.547527836004058 31.68653343934532 20.042394320606657 19 22.905189454530596 27.009959051297443 30.268884957087145 19.81596653708482 20 24.909884570541447 26.189099411382045 30.436388091878452 18.46462792619806 21 24.060741099235393 26.527876858483424 30.1757054458946 19.235676596386583 22 23.208769244790275 24.581163596823412 30.583149750316625 21.62691740806969 23 21.924054770068164 26.31527672586368 31.121328208722105 20.639340295346052 24 21.930182933536138 25.016891732636083 31.864721577106437 21.18820375672134 25 22.07348767924878 26.602200482082196 29.866626021753405 21.457685816915617 26 21.747280823876583 27.52001081070889 29.71216487588112 21.02054348953341 27 22.108685336090485 26.13504586694657 30.219859649343345 21.5364091476196 28 20.096290732645514 27.537295374336505 30.804392164750176 21.562021728267805 29 22.302429581116456 25.41317963689846 30.138622200293526 22.14576858169156 30 23.12831745772353 25.012806290324104 29.945506484854008 21.913369767098363 31 22.830551566138595 26.591986876302236 28.508844982605442 22.068616574953726 32 23.15455856795819 26.525677004930813 28.824366835007844 21.495397592103156 33 22.288916195007587 27.094339148279555 28.2365345392721 22.38021011744075 34 21.674528524243957 27.17039122824109 30.028786655059818 21.12629359245513 35 21.617489464272808 26.305220252480332 29.568231600582017 22.50905868266484 36 21.471199203024486 28.765599318673928 28.498788509222102 21.264412969079487 37 22.050389216946414 28.12811318560793 28.953843929818383 20.86765366762727 38 21.672800067881195 27.539180963095884 28.841180001445615 21.9468389675773 39 22.201864847283023 26.223668538637284 29.594944108006526 21.979522506073167 40 23.256066096171313 25.304129753647832 30.575607395279114 20.864196754901744 41 20.868910726800188 26.737805740360717 30.25254318783921 22.14074034499989 42 22.45736212417859 26.524105680964666 29.19394223184571 21.824589963011036 43 22.173423883495754 25.989069870491477 29.72410693802384 22.113399307988928 44 22.13838335905067 26.6204278400895 28.392252744317307 22.84893605654252 45 21.584334528587096 25.278045775809783 29.035395643661438 24.102224051941683 46 21.98329368359192 26.528191123276653 28.438292536525427 23.050222656606003 47 21.298196434351656 26.119961156871558 29.892867131988073 22.688975276788717 48 21.106652042878288 25.14998287256877 30.251286128666294 23.49207895588665 49 21.215073396542458 25.347184030320268 30.019044446469707 23.418698126667568 50 19.81345241873898 25.411136915742468 30.89945726470209 23.87595340081646 51 20.014267621612618 24.576606757321585 29.991389144665515 25.417736476400286 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 202.0 1 480.5 2 759.0 3 5527.0 4 10295.0 5 6790.0 6 3285.0 7 3163.0 8 3041.0 9 2997.5 10 2954.0 11 2902.5 12 2851.0 13 2712.5 14 2574.0 15 2544.5 16 2515.0 17 2352.0 18 2189.0 19 2265.0 20 2341.0 21 2553.0 22 2765.0 23 3008.5 24 3252.0 25 3754.5 26 5364.5 27 6472.0 28 7707.5 29 8943.0 30 10512.5 31 12082.0 32 13871.0 33 15660.0 34 17661.0 35 19662.0 36 21763.5 37 23865.0 38 25529.0 39 27193.0 40 29025.0 41 30857.0 42 33478.5 43 36100.0 44 38242.0 45 40384.0 46 44910.5 47 49437.0 48 52449.0 49 55461.0 50 54586.5 51 53712.0 52 48343.5 53 42975.0 54 39012.5 55 35050.0 56 31914.5 57 28779.0 58 27608.5 59 26438.0 60 25588.0 61 24738.0 62 22683.0 63 20628.0 64 16543.0 65 12458.0 66 10316.0 67 8174.0 68 6534.5 69 4895.0 70 4173.0 71 3451.0 72 2840.5 73 2230.0 74 1893.5 75 1246.5 76 936.0 77 687.5 78 439.0 79 364.5 80 290.0 81 212.5 82 135.0 83 95.5 84 56.0 85 43.0 86 30.0 87 26.0 88 22.0 89 14.5 90 7.0 91 8.5 92 10.0 93 5.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 636406.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.01745523617218 #Duplication Level Percentage of deduplicated Percentage of total 1 70.18577428787462 25.27912983629609 2 8.149448847270204 5.87044818112059 3 3.720120860283111 4.01967859675192 4 2.591066976519264 3.732945553628264 5 2.105545389967171 3.791819341543565 6 1.7873060340222628 3.862452904424237 7 1.6181897895303337 4.079815481562776 8 1.3961217027132005 4.022780074537695 9 1.2561147930810117 4.0717852498160125 >10 7.135429178312545 37.69935556534008 >50 0.03600268414181595 0.897428626555221 >100 0.014927942178415775 0.8934751952537651 >500 0.0035124569831566526 0.8382853115115413 >1k 0.0 0.0 >5k 4.390571228945816E-4 0.9406000816582999 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5948 0.9346234950644714 No Hit CGTTTCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTT 878 0.13796224422774142 No Hit CGCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCTG 793 0.12460599051548854 No Hit GCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCTGC 690 0.10842135366417036 No Hit CGTTCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTC 638 0.10025046904020389 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.5713239661473965E-4 0.0 0.0 0.10009333664358916 0.0 2 1.5713239661473965E-4 0.0 0.0 0.398330625418365 0.0 3 1.5713239661473965E-4 0.0 0.0 0.6821117337045848 0.0 4 1.5713239661473965E-4 0.0 0.0 0.986162921154106 0.0 5 1.5713239661473965E-4 0.0 0.0 1.8111080033814893 0.0 6 1.5713239661473965E-4 0.0 0.0 2.678478832694852 0.0 7 1.5713239661473965E-4 0.0 0.0 3.394374031671606 0.0 8 1.5713239661473965E-4 0.0 0.0 4.510485444826101 0.0 9 1.5713239661473965E-4 0.0 0.0 5.128801425505102 0.0 10 1.5713239661473965E-4 0.0 0.0 6.0114140972900945 0.0 11 1.5713239661473965E-4 0.0 0.0 6.954837006564992 0.0 12 1.5713239661473965E-4 0.0 0.0 7.7313853106350345 0.0 13 3.142647932294793E-4 0.0 0.0 8.087447321364035 0.0 14 3.142647932294793E-4 0.0 0.0 8.270035166230363 0.0 15 3.142647932294793E-4 0.0 0.0 8.501334054047259 0.0 16 3.142647932294793E-4 0.0 0.0 8.891650927238272 0.0 17 3.142647932294793E-4 0.0 0.0 9.386932241367932 0.0 18 3.142647932294793E-4 0.0 0.0 9.91332577002731 0.0 19 3.142647932294793E-4 0.0 0.0 10.268130721583391 0.0 20 3.142647932294793E-4 0.0 0.0 10.627021115451457 0.0 21 3.142647932294793E-4 0.0 0.0 11.063377780850589 0.0 22 4.7139718984421893E-4 0.0 0.0 11.550331077959667 0.0 23 4.7139718984421893E-4 0.0 0.0 12.035398786309369 0.0 24 4.7139718984421893E-4 0.0 0.0 12.422415879171472 0.0 25 4.7139718984421893E-4 0.0 0.0 12.766221562964523 0.0 26 4.7139718984421893E-4 0.0 0.0 13.089600035197657 0.0 27 6.285295864589586E-4 0.0 0.0 13.412821375034177 0.0 28 6.285295864589586E-4 0.0 0.0 13.757569853206915 0.0 29 6.285295864589586E-4 0.0 0.0 14.123845469715873 0.0 30 6.285295864589586E-4 0.0 0.0 14.555331030819948 0.0 31 6.285295864589586E-4 0.0 0.0 14.944705109631274 0.0 32 6.285295864589586E-4 0.0 0.0 15.33549338001213 0.0 33 6.285295864589586E-4 0.0 0.0 15.696583627432803 0.0 34 6.285295864589586E-4 0.0 0.0 16.0568882128704 0.0 35 6.285295864589586E-4 0.0 0.0 16.436362950694996 0.0 36 6.285295864589586E-4 0.0 0.0 16.82165158719434 0.0 37 6.285295864589586E-4 0.0 0.0 17.212754122368427 0.0 38 6.285295864589586E-4 0.0 0.0 17.630097767777173 0.0 39 6.285295864589586E-4 0.0 0.0 18.05404097384374 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCACGC 20 7.030911E-4 45.0 22 TCTAGCG 40 6.8066583E-9 45.0 1 CTAACGG 20 7.030911E-4 45.0 2 CGTAGTA 20 7.030911E-4 45.0 38 TTGACCG 25 3.888838E-5 44.999996 1 GTATACG 25 3.888838E-5 44.999996 1 GCGTAAG 50 2.1827873E-11 44.999996 1 CGTTTTT 3340 0.0 42.84431 1 TAGACGG 65 0.0 41.538464 2 TATACGG 60 3.6379788E-12 41.250004 2 ACGGGCC 55 6.002665E-11 40.909092 5 GCGTACG 50 1.0804797E-9 40.499996 1 GACACGA 185 0.0 40.135136 25 CACGACC 205 0.0 39.512196 27 GCGTTAG 75 0.0 39.0 1 ACGTACG 35 6.2438958E-6 38.571426 1 GTAAGCG 35 6.2438958E-6 38.571426 1 GCGAGAC 200 0.0 38.249996 21 TCAAGCG 200 0.0 38.249996 17 AGGGATC 530 0.0 38.207546 6 >>END_MODULE