FastQCFastQC Report
Sat 18 Jun 2016
SRR3550527_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550527_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464985
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTC35720.768196823553448No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGC31100.6688387797455831No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCG28840.6202350613460649No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19270.41442197060120217No Hit
GCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGC12320.2649547834876394TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGC8170.17570459262126734TruSeq Adapter, Index 13 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCT7260.15613406884093037No Hit
CTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGCT6740.1449509123950235TruSeq Adapter, Index 16 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCT6060.13032678473499146No Hit
CGCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTG5470.11763820338290482No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT5470.11763820338290482No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTC4680.10064840801316173No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAG207.028949E-445.01
CGCGCTT207.028949E-445.042
ACGGGTC207.028949E-445.05
TGCGACG207.028949E-445.01
GCGATAT207.028949E-445.09
TACTCGA207.028949E-445.01
TATAGCG351.2098462E-745.01
GTACGAG207.028949E-445.01
TCGCAAG302.1627038E-644.9999961
CGGTCTA302.1627038E-644.99999631
CGTTTTT10550.041.800951
TACGGGT700.041.7857134
CGAATAG1150.041.0869626
GCGATAA501.0786607E-940.59
TCACGAC451.924127E-840.025
CGTCGGG451.924127E-840.03
AACGAAT1250.039.60000224
CGACGGT356.2402687E-638.57142628
ACGGTCT356.2402687E-638.57142630
TACGGCT3800.037.8947377
Produced by FastQC (version 0.11.5)