##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550527_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	464985
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.2409733647322	33.0	31.0	34.0	30.0	34.0
2	32.3422411475639	34.0	31.0	34.0	30.0	34.0
3	32.438618450057525	34.0	31.0	34.0	30.0	34.0
4	35.953723238384036	37.0	35.0	37.0	35.0	37.0
5	35.90802068883942	37.0	35.0	37.0	35.0	37.0
6	35.91229394496597	37.0	35.0	37.0	35.0	37.0
7	36.06671613062787	37.0	35.0	37.0	35.0	37.0
8	36.01192081464993	37.0	35.0	37.0	35.0	37.0
9	37.63175371248535	39.0	38.0	39.0	35.0	39.0
10	37.42259642784176	39.0	37.0	39.0	35.0	39.0
11	37.36751938234567	39.0	37.0	39.0	34.0	39.0
12	37.26631181651021	39.0	37.0	39.0	34.0	39.0
13	37.193750336032345	39.0	37.0	39.0	34.0	39.0
14	38.40230545071346	40.0	38.0	41.0	34.0	41.0
15	38.428691248104776	40.0	38.0	41.0	34.0	41.0
16	38.493282579007925	40.0	38.0	41.0	34.0	41.0
17	38.479628375108874	40.0	38.0	41.0	34.0	41.0
18	38.430426787960904	40.0	38.0	41.0	34.0	41.0
19	38.387272707721756	40.0	37.0	41.0	34.0	41.0
20	38.22934933384948	40.0	37.0	41.0	34.0	41.0
21	38.241347570351735	40.0	37.0	41.0	34.0	41.0
22	38.229628912760624	40.0	37.0	41.0	34.0	41.0
23	38.19434605417379	40.0	37.0	41.0	34.0	41.0
24	38.168624794348204	40.0	37.0	41.0	34.0	41.0
25	38.12135445229416	40.0	37.0	41.0	34.0	41.0
26	37.95531683817757	40.0	36.0	41.0	34.0	41.0
27	37.88313816574728	40.0	36.0	41.0	33.0	41.0
28	37.72099530092368	40.0	36.0	41.0	33.0	41.0
29	37.57971762529974	40.0	36.0	41.0	33.0	41.0
30	37.590191081432735	40.0	36.0	41.0	33.0	41.0
31	37.415439207716375	40.0	36.0	41.0	32.0	41.0
32	37.231506392679336	40.0	35.0	41.0	32.0	41.0
33	37.15135542006731	39.0	35.0	41.0	31.0	41.0
34	36.85747282170393	39.0	35.0	41.0	30.0	41.0
35	36.86657203995828	39.0	35.0	41.0	31.0	41.0
36	36.69609342236846	39.0	35.0	41.0	30.0	41.0
37	36.753422153402795	39.0	35.0	41.0	30.0	41.0
38	36.715984386593114	39.0	35.0	41.0	30.0	41.0
39	36.68920287751218	39.0	35.0	41.0	30.0	41.0
40	36.63895394475091	39.0	35.0	41.0	30.0	41.0
41	36.53874856178156	39.0	35.0	41.0	30.0	41.0
42	36.473922814714456	39.0	35.0	41.0	30.0	41.0
43	36.401705431358	39.0	35.0	41.0	30.0	41.0
44	36.22402658150263	39.0	35.0	41.0	30.0	41.0
45	36.197341849737086	39.0	35.0	41.0	30.0	41.0
46	36.07636590427648	39.0	35.0	41.0	30.0	41.0
47	35.978498231125734	38.0	35.0	40.0	29.0	41.0
48	35.8792219103842	38.0	35.0	40.0	29.0	41.0
49	35.8418766196759	38.0	35.0	40.0	29.0	41.0
50	35.76210200329043	38.0	35.0	40.0	28.0	41.0
51	34.49990644859512	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	3.0
12	3.0
13	1.0
14	9.0
15	10.0
16	22.0
17	43.0
18	89.0
19	204.0
20	335.0
21	614.0
22	941.0
23	1450.0
24	2004.0
25	2781.0
26	3883.0
27	4608.0
28	5285.0
29	6051.0
30	7500.0
31	9222.0
32	11829.0
33	15995.0
34	26603.0
35	36292.0
36	34198.0
37	51638.0
38	92107.0
39	151134.0
40	128.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.218964052603845	21.59510521844791	25.023387851221006	12.162542877727239
2	30.316461821349076	28.012086411389614	25.279955267374216	16.391496499887094
3	30.30936481821994	27.544974569072124	26.239771175414262	15.905889437293677
4	27.343462692344918	25.291998666623655	29.763540759379335	17.6009978816521
5	27.137864662300938	29.59536329128897	24.55842661591234	18.708345430497758
6	25.55652332870953	36.04890480338076	23.688721141542203	14.705850726367517
7	79.1849199436541	5.807714227340667	10.599911825112638	4.407454003892599
8	79.7909609987419	7.303031280578944	8.42371259287934	4.482295127799821
9	73.50366140843254	7.846274611008957	9.997526801939847	8.652537178618665
10	38.792004043141176	30.673462584814565	16.680107960471844	13.854425411572416
11	29.100723679258472	24.542942245448778	27.06732475241137	19.289009322881384
12	26.362570835618353	23.429357936277516	29.49492994397669	20.713141284127445
13	24.18443605707711	24.29820316784412	32.10942288460918	19.407937890469583
14	20.823037302278568	28.490811531554783	29.002010817553252	21.684140348613397
15	19.965160166456982	26.015462864393474	33.74560469692571	20.273772272223837
16	22.4002924825532	26.6888179188576	29.261804144219706	21.649085454369494
17	21.81597255825457	26.453326451390907	28.87361957912621	22.857081411228318
18	22.22953428605224	26.78107895953633	29.167177435831267	21.822209318580168
19	23.038807703474305	28.016817746809036	27.053560867554864	21.890813682161788
20	25.07758314784348	26.999150510231512	28.975558351344667	18.94770799058034
21	23.686785595234255	28.906738927062165	28.012731593492262	19.393743884211318
22	22.84181210146564	25.20210329365463	27.7781003688291	24.177984236050627
23	22.14673591621235	28.460487972730302	27.652074798111766	21.74070131294558
24	22.695355764164436	25.506844306805597	28.811897157972837	22.98590277105713
25	21.446283213436992	30.01301117240341	26.17955417916707	22.361151434992525
26	21.138101229071907	28.298117143563772	27.69164596707421	22.872135660290116
27	22.262868694689075	27.769928062195554	27.5327161091218	22.43448713399357
28	19.339548587588848	28.123272793746036	29.266535479639128	23.27064313902599
29	21.478542318569417	26.314827360022363	28.521780272481905	23.684850048926307
30	21.62263298816091	27.046678925126617	29.897738636730214	21.432949449982257
31	22.506747529490198	26.645160596578382	26.200630127853586	24.64746174607783
32	21.86715700506468	27.785197371958237	27.567340881963936	22.78030474101315
33	21.134230136456015	26.06686237190447	28.031226813768185	24.76768067787133
34	20.114412292869662	26.684946826241706	27.946492897620352	25.254147983268275
35	19.746873555060915	25.426196543974537	28.889749131692422	25.93718076927213
36	19.698915018764048	27.74519608159403	27.084314547781112	25.471574351860816
37	20.000430121401767	26.339559340623893	29.785261890168503	23.874748647805845
38	19.49890856694302	25.732442982031678	28.239835693624528	26.52881275740078
39	21.20412486424293	25.229200941965868	28.63619256535157	24.930481628439626
40	21.00906480854221	24.22852350075809	30.16763981633816	24.594771874361538
41	19.257610460552492	26.174177661644997	26.276546555265224	28.291665322537284
42	20.269255997505294	25.487703904427022	28.533608611030463	25.709431487037214
43	21.79941288428659	24.800154843704636	27.892727722399645	25.507704549609127
44	21.108422852350074	24.771766831188103	28.276396012774608	25.84341430368722
45	20.899598911792854	24.073034614019807	27.849500521522195	27.177865952665144
46	22.289321160897664	26.09202447390776	27.286471606610966	24.33218275858361
47	19.34126907319591	24.862522446960654	30.990677118616727	24.805531361226706
48	20.322375990623353	24.2584169381808	29.234491435207588	26.18471563598826
49	20.720668408658344	23.05343183113434	31.06207727130983	25.16382248889749
50	19.662354699613964	23.326558921255526	30.52765142961601	26.4834349495145
51	19.62751486607095	23.291504027011626	28.123702915147803	28.957278191769625
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	131.0
1	336.0
2	541.0
3	1879.0
4	3217.0
5	2138.0
6	1059.0
7	1060.5
8	1062.0
9	1042.5
10	1023.0
11	1043.5
12	1064.0
13	1086.5
14	1109.0
15	1082.0
16	1055.0
17	1081.5
18	1108.0
19	1107.0
20	1106.0
21	1310.5
22	1515.0
23	1491.0
24	1467.0
25	2093.0
26	3163.0
27	3607.0
28	4310.0
29	5013.0
30	6169.5
31	7326.0
32	8283.0
33	9240.0
34	11215.0
35	13190.0
36	13867.0
37	14544.0
38	16016.5
39	17489.0
40	19252.5
41	21016.0
42	23583.5
43	26151.0
44	29395.0
45	32639.0
46	43030.5
47	53422.0
48	50516.0
49	47610.0
50	45800.5
51	43991.0
52	38320.0
53	32649.0
54	28506.5
55	24364.0
56	22549.0
57	20734.0
58	19272.5
59	17811.0
60	17174.5
61	16538.0
62	14708.5
63	12879.0
64	11255.5
65	9632.0
66	7808.5
67	5985.0
68	4981.5
69	3978.0
70	3323.0
71	2668.0
72	2168.5
73	1669.0
74	1434.0
75	911.0
76	623.0
77	504.0
78	385.0
79	355.0
80	325.0
81	192.5
82	60.0
83	51.5
84	43.0
85	28.0
86	13.0
87	10.0
88	7.0
89	5.5
90	4.0
91	4.5
92	5.0
93	2.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	464985.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.01560807446463
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.80417385350917	26.948907655466808
2	8.742567486954638	6.4722290332334
3	3.4200872288362527	3.797898253292537
4	2.1503868584545742	3.1839150864413504
5	1.609513882458544	2.9788567531747696
6	1.3797086020798555	3.0642451722933237
7	1.1973321726033324	3.102398490522253
8	1.0541715084056364	3.121663951872819
9	0.9932541593810932	3.3089316013784136
>10	6.57130171302857	37.59159881757955
>50	0.05186259813280981	1.333670047548458
>100	0.019229952116659815	1.5060139530945063
>500	0.0034963549303017845	0.8448949662376368
>1k	0.0029136291085848204	2.7447762178641635
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTC	3572	0.768196823553448	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGC	3110	0.6688387797455831	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCG	2884	0.6202350613460649	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1927	0.41442197060120217	No Hit
GCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGC	1232	0.2649547834876394	TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGC	817	0.17570459262126734	TruSeq Adapter, Index 13 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCT	726	0.15613406884093037	No Hit
CTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGCT	674	0.1449509123950235	TruSeq Adapter, Index 16 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCT	606	0.13032678473499146	No Hit
CGCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTG	547	0.11763820338290482	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	547	0.11763820338290482	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTC	468	0.10064840801316173	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1506070088282418E-4	0.0	0.0	0.16796240738948567	0.0
2	2.1506070088282418E-4	0.0	0.0	0.81099390302913	0.0
3	2.1506070088282418E-4	0.0	0.0	1.2088561996623548	0.0
4	4.3012140176564836E-4	0.0	0.0	1.7583362904179705	0.0
5	4.3012140176564836E-4	0.0	0.0	3.6513005795885887	0.0
6	4.3012140176564836E-4	0.0	0.0	4.60789057711539	0.0
7	4.3012140176564836E-4	0.0	0.0	5.5603944213254195	0.0
8	4.3012140176564836E-4	0.0	0.0	6.925814811230469	0.0
9	4.3012140176564836E-4	0.0	0.0	7.450777982085444	0.0
10	4.3012140176564836E-4	0.0	0.0	9.117283353226448	0.0
11	4.3012140176564836E-4	0.0	0.0	10.424207232491371	0.0
12	4.3012140176564836E-4	0.0	0.0	12.219103842059422	0.0
13	4.3012140176564836E-4	0.0	0.0	12.71933503231287	0.0
14	4.3012140176564836E-4	0.0	0.0	12.968590384636064	0.0
15	4.3012140176564836E-4	0.0	0.0	13.378926201920493	0.0
16	4.3012140176564836E-4	0.0	0.0	14.015935997935417	0.0
17	4.3012140176564836E-4	0.0	0.0	14.771444240136779	0.0
18	4.3012140176564836E-4	0.0	0.0	15.557921223265266	0.0
19	4.3012140176564836E-4	0.0	0.0	16.16525264255836	0.0
20	4.3012140176564836E-4	0.0	0.0	16.682258567480673	0.0
21	6.451821026484725E-4	0.0	0.0	17.308515328451456	0.0
22	6.451821026484725E-4	0.0	0.0	17.980149897308515	0.0
23	8.602428035312967E-4	0.0	0.0	18.64232179532673	0.0
24	8.602428035312967E-4	0.0	0.0	19.186425368560275	0.0
25	8.602428035312967E-4	0.0	0.0	19.684505951804898	0.0
26	8.602428035312967E-4	0.0	0.0	20.133337634547352	0.0
27	8.602428035312967E-4	0.0	0.0	20.59464283794101	0.0
28	8.602428035312967E-4	0.0	0.0	21.04239921717905	0.0
29	8.602428035312967E-4	0.0	0.0	21.50327429917094	0.0
30	8.602428035312967E-4	0.0	0.0	22.078776734733378	0.0
31	0.0010753035044141208	0.0	0.0	22.564168736625913	0.0
32	0.0010753035044141208	0.0	0.0	23.057733045151995	0.0
33	0.0010753035044141208	0.0	0.0	23.519253309246533	0.0
34	0.0010753035044141208	0.0	0.0	23.97647235932342	0.0
35	0.0010753035044141208	0.0	0.0	24.467671000139788	0.0
36	0.0010753035044141208	0.0	0.0	24.94123466348377	0.0
37	0.0010753035044141208	0.0	0.0	25.41436820542598	0.0
38	0.0010753035044141208	0.0	0.0	25.888146929470842	0.0
39	0.0010753035044141208	0.0	0.0	26.37375399206426	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAG	20	7.028949E-4	45.0	1
CGCGCTT	20	7.028949E-4	45.0	42
ACGGGTC	20	7.028949E-4	45.0	5
TGCGACG	20	7.028949E-4	45.0	1
GCGATAT	20	7.028949E-4	45.0	9
TACTCGA	20	7.028949E-4	45.0	1
TATAGCG	35	1.2098462E-7	45.0	1
GTACGAG	20	7.028949E-4	45.0	1
TCGCAAG	30	2.1627038E-6	44.999996	1
CGGTCTA	30	2.1627038E-6	44.999996	31
CGTTTTT	1055	0.0	41.80095	1
TACGGGT	70	0.0	41.785713	4
CGAATAG	115	0.0	41.08696	26
GCGATAA	50	1.0786607E-9	40.5	9
TCACGAC	45	1.924127E-8	40.0	25
CGTCGGG	45	1.924127E-8	40.0	3
AACGAAT	125	0.0	39.600002	24
CGACGGT	35	6.2402687E-6	38.571426	28
ACGGTCT	35	6.2402687E-6	38.571426	30
TACGGCT	380	0.0	37.894737	7
>>END_MODULE