FastQCFastQC Report
Sat 18 Jun 2016
SRR3550523_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550523_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences536136
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12950.24154319053374518No Hit
GCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTGC10390.19379411194174612No Hit
CCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTGC10090.18819851679424623No Hit
CTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTGCT7820.14585851351149706No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATAT207.0299144E-445.00000414
TTAGGCG253.8880113E-545.01
GTCGAAG502.1827873E-1145.01
TAGACCG253.8880113E-545.01
TAACGCC253.8880113E-545.012
ATGGTCG253.8880113E-545.044
GCTACGA253.8880113E-545.010
CCGCATC253.8880113E-545.020
ACGTTAG302.163326E-644.9999961
GAATAAC302.163326E-644.9999969
TTAAGCG302.163326E-644.9999961
TACGAAT302.163326E-644.99999612
CGTATGG850.042.352942
ACGTAGG556.002665E-1140.9090922
AGGGCGC556.002665E-1140.9090926
GCGAGAC1050.040.71428721
AGTTGCG451.9250365E-840.01
TCGAAGG900.040.02
CGTTTTT7300.039.7602731
TATTGCG403.4536242E-739.3750041
Produced by FastQC (version 0.11.5)