SRR3550520_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550520_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	416352
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGC	782	0.1878218430558758	TruSeq Adapter, Index 21 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGC	586	0.14074629159941587	TruSeq Adapter, Index 21 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	585	0.14050611021443393	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	452	0.10856198601183614	No Hit
CTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGCT	451	0.10832180462685422	Illumina Paired End PCR Primer 2 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	445	0.10688071631696258	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTATG	20	7.028098E-4	45.0	43
CGACGGT	25	3.886505E-5	45.0	28
CACGCCA	25	3.886505E-5	45.0	27
ATATGCG	20	7.028098E-4	45.0	1
CTACGAA	45	3.8380676E-10	45.0	11
TGCGACG	20	7.028098E-4	45.0	1
GTCAACG	20	7.028098E-4	45.0	1
TCGTACA	20	7.028098E-4	45.0	34
ACGTCAC	20	7.028098E-4	45.0	35
ATAACGC	20	7.028098E-4	45.0	11
ACGTAGG	20	7.028098E-4	45.0	2
GTACGAG	20	7.028098E-4	45.0	1
TACGAAT	50	1.0786607E-9	40.5	12
CGAATAT	45	1.9232175E-8	40.0	14
GCGATGA	75	0.0	39.0	9
GGCGATT	185	0.0	38.918922	8
AGGGTCA	105	0.0	38.57143	6
TTGTGCG	35	6.2386953E-6	38.571426	1
AAGCACG	35	6.2386953E-6	38.571426	1
ACTACGG	35	6.2386953E-6	38.571426	2