FastQCFastQC Report
Sat 18 Jun 2016
SRR3550517_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550517_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503832
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGC10990.21812826497721463TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7480.14846218580796774No Hit
CCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGC7250.14389717207323077TruSeq Adapter, Index 13 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT6770.13437018688769273No Hit
CTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGCT5870.11650708966480891TruSeq Adapter, Index 16 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGAG207.0295105E-445.01
ACGCACT207.0295105E-445.034
TCTACGG406.8012014E-945.02
GCGATCT351.2101191E-745.09
CATCGCG207.0295105E-445.01
TGTAGCG253.8876766E-545.01
CGTTTCA207.0295105E-445.025
AGTACGG253.8876766E-545.02
ATCGACG207.0295105E-445.01
CTTACGG207.0295105E-445.02
TTACGGG1000.042.753
TGATTCG650.041.5384615
TACGAAT1200.041.25000412
CGAATAT1200.041.25000414
CATACGA800.039.37518
CGACTGG403.4530603E-739.3752
GACACGA1450.038.79310625
ACACGAC1400.038.57142626
CACGACC1400.038.57142627
ACGGGTC356.2413055E-638.5714265
Produced by FastQC (version 0.11.5)