##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550517_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	503832
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30167397068864	33.0	31.0	34.0	30.0	34.0
2	32.41439805331936	34.0	31.0	34.0	30.0	34.0
3	32.51432620397275	34.0	31.0	34.0	31.0	34.0
4	35.997044649803904	37.0	35.0	37.0	35.0	37.0
5	35.95448085869893	37.0	35.0	37.0	35.0	37.0
6	35.96474420044777	37.0	35.0	37.0	35.0	37.0
7	36.12512504168056	37.0	35.0	37.0	35.0	37.0
8	36.10394337795138	37.0	35.0	37.0	35.0	37.0
9	37.661246209053814	39.0	38.0	39.0	35.0	39.0
10	37.43054629320885	39.0	37.0	39.0	35.0	39.0
11	37.381670874418454	39.0	37.0	39.0	35.0	39.0
12	37.27267620953016	39.0	37.0	39.0	34.0	39.0
13	37.20051525111545	39.0	37.0	39.0	34.0	39.0
14	38.40740167357373	40.0	38.0	41.0	34.0	41.0
15	38.49247963606916	40.0	38.0	41.0	34.0	41.0
16	38.52309103034345	40.0	38.0	41.0	34.0	41.0
17	38.55535376871656	40.0	38.0	41.0	34.0	41.0
18	38.513057924069926	40.0	38.0	41.0	34.0	41.0
19	38.466391574969435	40.0	37.0	41.0	34.0	41.0
20	38.350795503262994	40.0	37.0	41.0	34.0	41.0
21	38.314640594483876	40.0	37.0	41.0	34.0	41.0
22	38.28747082360787	40.0	37.0	41.0	34.0	41.0
23	38.25818725289382	40.0	37.0	41.0	34.0	41.0
24	38.23280180695152	40.0	37.0	41.0	34.0	41.0
25	38.19846893408914	40.0	37.0	41.0	34.0	41.0
26	38.07087283062608	40.0	37.0	41.0	34.0	41.0
27	38.008272598802776	40.0	37.0	41.0	34.0	41.0
28	37.932568792772194	40.0	36.0	41.0	33.0	41.0
29	37.89573508629861	40.0	36.0	41.0	33.0	41.0
30	37.88506089331364	40.0	36.0	41.0	33.0	41.0
31	37.79205767001699	40.0	36.0	41.0	33.0	41.0
32	37.65941623398275	40.0	36.0	41.0	33.0	41.0
33	37.57404849235459	40.0	36.0	41.0	33.0	41.0
34	37.44170278981883	40.0	36.0	41.0	33.0	41.0
35	37.43900149256101	40.0	36.0	41.0	33.0	41.0
36	37.29965544070246	40.0	35.0	41.0	32.0	41.0
37	37.28014695374649	40.0	35.0	41.0	32.0	41.0
38	37.189880753902095	40.0	35.0	41.0	32.0	41.0
39	37.123787294177426	39.0	35.0	41.0	32.0	41.0
40	37.04811722955271	39.0	35.0	41.0	31.0	41.0
41	36.929956811000494	39.0	35.0	41.0	31.0	41.0
42	36.854475301290904	39.0	35.0	41.0	31.0	41.0
43	36.796114577875166	39.0	35.0	41.0	31.0	41.0
44	36.63162323949253	39.0	35.0	41.0	31.0	41.0
45	36.57285166484066	39.0	35.0	41.0	31.0	41.0
46	36.454504676161896	39.0	35.0	41.0	30.0	41.0
47	36.355098524905124	39.0	35.0	41.0	30.0	41.0
48	36.22179615427365	38.0	35.0	40.0	30.0	41.0
49	36.1313036885311	38.0	35.0	40.0	30.0	41.0
50	36.05149137013925	38.0	35.0	40.0	30.0	41.0
51	34.8273154543578	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	7.0
11	3.0
12	2.0
13	7.0
14	2.0
15	14.0
16	24.0
17	56.0
18	113.0
19	203.0
20	329.0
21	488.0
22	809.0
23	1144.0
24	1794.0
25	2417.0
26	3362.0
27	4144.0
28	4876.0
29	5803.0
30	7250.0
31	9241.0
32	11717.0
33	15918.0
34	28336.0
35	38981.0
36	35844.0
37	53866.0
38	96128.0
39	180812.0
40	139.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.66977881516061	21.256093300941583	25.598215278108576	10.475912605789231
2	31.540275329871864	25.111346639355975	25.20522713920513	18.143150891567032
3	31.22409850902682	25.160966353863984	26.480453802061003	17.13448133504819
4	28.723860334397177	26.26351641023198	25.495800187364043	19.516823068006797
5	25.819916162530365	30.419068260848853	23.57154765874339	20.189467917877387
6	27.114196795757316	34.775679194652184	23.064831134187585	15.045292875402913
7	81.95152352371426	6.239579859953317	7.28536496292415	4.523531653408279
8	82.79168452976388	5.544903856841169	7.097603963225837	4.565807650169104
9	75.42633258705283	8.262674859873927	9.774091363787928	6.5369011892853175
10	40.028223693612155	28.177646517092995	16.612878896139982	15.18125089315486
11	31.22628177646517	26.02315851315518	24.05146953746487	18.699090172914783
12	28.383072135156162	24.3384699661792	27.148335159338828	20.130122739325806
13	25.24670922053383	25.016672224074693	29.032098000920943	20.704520554470538
14	20.940511916670637	28.60417758300386	28.13556899919021	22.319741501135297
15	20.345273821432542	26.462590704838117	32.85499928547611	20.337136188253226
16	22.779220057479478	25.62024643135013	30.855920227377382	20.744613283793008
17	22.470188475523585	25.953889391701995	27.34602010193874	24.229902030835675
18	24.23347465028025	25.816145064227758	27.869805808285303	22.08057447720669
19	25.07085695231744	27.28746883881929	26.024150907445335	21.617523301417933
20	27.74932914145985	26.05253338414392	26.246050270725164	19.952087203671066
21	25.756402927960114	26.704337953921147	26.542577684625034	20.996681433493706
22	23.860532876038043	26.34132012258054	25.494013877641752	24.30413312373966
23	23.6521300751044	27.235665856872927	26.379031105606632	22.73317296241604
24	23.316502326172216	25.457295288905822	28.254854792867462	22.971347592054496
25	23.02533384143921	27.263452896997414	26.190476190476193	23.52073707108719
26	22.61547499960304	28.390614331761384	26.295074548659077	22.6988361199765
27	24.085210943330317	27.244597405484367	26.742644373521333	21.927547277663983
28	21.021888248463775	28.164745391320917	28.137553787770525	22.675812572444784
29	22.52675495006272	25.77803712348561	27.456175868146527	24.23903205830515
30	23.918885660299466	25.65617110465393	26.83434160593214	23.590601629114467
31	23.248225599009192	27.35376077740199	25.60655139014592	23.791462233442896
32	24.04491973514981	26.253195509614315	27.090180853935436	22.611703901300434
33	23.428047444386223	27.817605868622874	25.31399355340669	23.44035313358421
34	21.652654059289606	26.083099128280857	28.18062369996348	24.08362311246606
35	22.07362771717557	25.152034805252544	27.37896759237206	25.39536988519983
36	21.475015481350926	28.37791168484733	26.451277409930295	23.69579542387145
37	22.541045427841027	27.379364550088127	27.81046062973372	22.269129392337128
38	21.494069451722	27.029049365661567	27.89560805982947	23.58127312278696
39	22.834397180012385	25.79590022070849	27.18525222693279	24.18445037234634
40	23.043990854094222	24.98332777592531	28.623231553374932	23.349449816605535
41	20.609250702615157	26.422497975515647	27.40596071706442	25.562290604804776
42	22.4219581130218	26.488392956382285	27.367455818606203	23.72219311198971
43	22.946934692516553	26.135497546801318	27.958525857825624	22.95904190285651
44	22.257816097429302	26.223026723193442	27.018529986185875	24.50062719319138
45	21.812628018863432	24.475618857079343	26.959581765350354	26.75217135870687
46	21.825727623493545	26.347472967179534	26.857960590037948	24.96883881928897
47	21.47124438304832	26.14700932056717	28.6639197192715	23.717826577113005
48	21.236840851712476	25.218922180409344	28.451547341177218	25.092689626700963
49	21.524238238142875	24.914058654472125	28.984860032709314	24.576843074675686
50	20.041601168643517	25.136950412042108	28.903880658632243	25.917567760682132
51	20.1827196367043	24.431755029454262	27.32438590641325	28.061139427428188
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	136.0
1	419.0
2	702.0
3	1132.0
4	1562.0
5	1140.5
6	719.0
7	730.5
8	742.0
9	751.0
10	760.0
11	770.5
12	781.0
13	810.5
14	840.0
15	860.0
16	880.0
17	1007.0
18	1134.0
19	1107.0
20	1080.0
21	1226.0
22	1372.0
23	1564.5
24	1757.0
25	2058.5
26	3238.0
27	4116.0
28	4738.5
29	5361.0
30	6415.5
31	7470.0
32	9089.5
33	10709.0
34	12306.5
35	13904.0
36	14670.0
37	15436.0
38	16655.5
39	17875.0
40	20416.0
41	22957.0
42	25434.0
43	27911.0
44	30363.5
45	32816.0
46	36529.0
47	40242.0
48	44292.5
49	48343.0
50	47495.0
51	46647.0
52	42239.5
53	37832.0
54	34327.0
55	30822.0
56	28316.0
57	25810.0
58	25296.0
59	24782.0
60	23956.5
61	23131.0
62	20504.0
63	17877.0
64	14876.5
65	11876.0
66	10284.0
67	8692.0
68	7080.5
69	5469.0
70	4567.5
71	3666.0
72	2985.5
73	2305.0
74	1834.5
75	1088.0
76	812.0
77	612.0
78	412.0
79	296.0
80	180.0
81	151.5
82	123.0
83	77.5
84	32.0
85	25.5
86	19.0
87	13.5
88	8.0
89	5.0
90	2.0
91	4.0
92	6.0
93	3.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	503832.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.05207844524271
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.74105763902116	22.99450007190585
2	9.222308141876075	5.911882880192249
3	3.259787218448478	3.1344886692153047
4	1.7919000955462414	2.2973648971394414
5	1.3102421976470124	2.0997992850624625
6	1.0858398376538225	2.088205419326996
7	0.9305653190447198	2.087858682311057
8	0.8799872897953486	2.2564337314669634
9	0.8243734488122295	2.378059420455437
>10	8.862627841495536	51.22283432867477
>50	0.06397979576813317	1.4145747573346716
>100	0.0242253595626912	1.350266854925228
>500	0.002484652262840123	0.5449248572589721
>1k	6.211630657100307E-4	0.21880614473058468
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGC	1099	0.21812826497721463	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	748	0.14846218580796774	No Hit
CCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGC	725	0.14389717207323077	TruSeq Adapter, Index 13 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	677	0.13437018688769273	No Hit
CTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGCT	587	0.11650708966480891	TruSeq Adapter, Index 16 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.9695771606408485E-4	0.0	0.0	0.13159148287524414	0.0
2	3.9695771606408485E-4	0.0	0.0	0.6819733561980977	0.0
3	3.9695771606408485E-4	0.0	0.0	0.9622255037393417	0.0
4	3.9695771606408485E-4	0.0	0.0	1.3079756744311597	0.0
5	3.9695771606408485E-4	0.0	0.0	2.361302974007209	0.0
6	3.9695771606408485E-4	0.0	0.0	3.0395052319026976	0.0
7	3.9695771606408485E-4	0.0	0.0	3.728028390415853	0.0
8	3.9695771606408485E-4	0.0	0.0	4.889526667619365	0.0
9	3.9695771606408485E-4	0.0	0.0	5.342653900506518	0.0
10	3.9695771606408485E-4	0.0	0.0	6.275901490973181	0.0
11	3.9695771606408485E-4	0.0	0.0	7.42052906524397	0.0
12	3.9695771606408485E-4	0.0	0.0	8.362509725464044	0.0
13	3.9695771606408485E-4	0.0	0.0	8.732077359119707	0.0
14	5.954365740961273E-4	0.0	0.0	8.879745629495547	0.0
15	5.954365740961273E-4	0.0	0.0	9.152852538147636	0.0
16	5.954365740961273E-4	0.0	0.0	9.705814636624906	0.0
17	5.954365740961273E-4	0.0	0.0	10.39116213340955	0.0
18	5.954365740961273E-4	0.0	0.0	11.080677686212864	0.0
19	5.954365740961273E-4	0.0	0.0	11.503437653821114	0.0
20	5.954365740961273E-4	0.0	0.0	11.937709395195224	0.0
21	5.954365740961273E-4	0.0	0.0	12.482335381635147	0.0
22	5.954365740961273E-4	0.0	0.0	13.097818320392511	0.0
23	7.939154321281697E-4	0.0	0.0	13.694247288778799	0.0
24	7.939154321281697E-4	0.0	0.0	14.173176773210118	0.0
25	7.939154321281697E-4	0.0	0.0	14.5911732482256	0.0
26	7.939154321281697E-4	0.0	0.0	14.963321107035679	0.0
27	7.939154321281697E-4	0.0	0.0	15.35690468251322	0.0
28	7.939154321281697E-4	0.0	0.0	15.754854792867464	0.0
29	7.939154321281697E-4	0.0	0.0	16.175629971895393	0.0
30	7.939154321281697E-4	0.0	0.0	16.685720637037743	0.0
31	7.939154321281697E-4	0.0	0.0	17.139641785357025	0.0
32	9.923942901602122E-4	0.0	0.0	17.5637911049715	0.0
33	9.923942901602122E-4	0.0	0.0	17.978611918258466	0.0
34	0.0011908731481922545	0.0	0.0	18.39680687213198	0.0
35	0.0011908731481922545	0.0	0.0	18.839414725543435	0.0
36	0.0011908731481922545	0.0	0.0	19.28400736753521	0.0
37	0.001389352006224297	0.0	0.0	19.71847758776735	0.0
38	0.001389352006224297	0.0	0.0	20.13349687991235	0.0
39	0.001389352006224297	0.0	0.0	20.55228727035996	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGAG	20	7.0295105E-4	45.0	1
ACGCACT	20	7.0295105E-4	45.0	34
TCTACGG	40	6.8012014E-9	45.0	2
GCGATCT	35	1.2101191E-7	45.0	9
CATCGCG	20	7.0295105E-4	45.0	1
TGTAGCG	25	3.8876766E-5	45.0	1
CGTTTCA	20	7.0295105E-4	45.0	25
AGTACGG	25	3.8876766E-5	45.0	2
ATCGACG	20	7.0295105E-4	45.0	1
CTTACGG	20	7.0295105E-4	45.0	2
TTACGGG	100	0.0	42.75	3
TGATTCG	65	0.0	41.53846	15
TACGAAT	120	0.0	41.250004	12
CGAATAT	120	0.0	41.250004	14
CATACGA	80	0.0	39.375	18
CGACTGG	40	3.4530603E-7	39.375	2
GACACGA	145	0.0	38.793106	25
ACACGAC	140	0.0	38.571426	26
CACGACC	140	0.0	38.571426	27
ACGGGTC	35	6.2413055E-6	38.571426	5
>>END_MODULE