Basic Statistics
Measure | Value |
---|---|
Filename | SRR3550516_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 463438 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 47 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GAATCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTC | 3816 | 0.8234111143238145 | No Hit |
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGC | 3419 | 0.7377470125453675 | No Hit |
GAATGATACCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCG | 3054 | 0.6589878257717322 | No Hit |
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 1901 | 0.41019510700460476 | No Hit |
GCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTGC | 1454 | 0.313742075531139 | Illumina Single End Adapter 1 (95% over 21bp) |
CCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTGC | 915 | 0.1974374134188392 | Illumina Single End Adapter 1 (95% over 21bp) |
CTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTGCT | 748 | 0.1614023882374773 | Illumina Single End Adapter 1 (95% over 22bp) |
GAACTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCT | 741 | 0.1598919380801747 | No Hit |
GAATGACTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCT | 635 | 0.13701940712673538 | No Hit |
GAATGATCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTC | 512 | 0.11047864007698979 | No Hit |
CGCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTG | 511 | 0.11026286148308943 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGTTTCG | 30 | 2.1626875E-6 | 45.000004 | 1 |
ATTACGG | 35 | 1.209828E-7 | 45.000004 | 2 |
TAGCGCG | 30 | 2.1626875E-6 | 45.000004 | 1 |
CTCGATC | 30 | 2.1626875E-6 | 45.000004 | 16 |
CGATGAT | 35 | 1.209828E-7 | 45.000004 | 10 |
AGGGCGC | 35 | 1.209828E-7 | 45.000004 | 6 |
CAAAACG | 20 | 7.0289243E-4 | 45.0 | 1 |
GAGTACG | 20 | 7.0289243E-4 | 45.0 | 1 |
TTCACCG | 20 | 7.0289243E-4 | 45.0 | 23 |
ACGATAG | 20 | 7.0289243E-4 | 45.0 | 1 |
CGAGACG | 25 | 3.8871898E-5 | 45.0 | 15 |
TATCGCG | 25 | 3.8871898E-5 | 45.0 | 1 |
TATGCGG | 60 | 3.6379788E-12 | 41.250004 | 2 |
ACAACGA | 55 | 6.002665E-11 | 40.909092 | 13 |
AACGAGC | 55 | 6.002665E-11 | 40.909092 | 15 |
CGAATAT | 55 | 6.002665E-11 | 40.909092 | 14 |
CGTTTTT | 1275 | 0.0 | 40.764706 | 1 |
ACACGAC | 45 | 1.9239451E-8 | 40.000004 | 26 |
TACGGGA | 135 | 0.0 | 40.0 | 4 |
TACGGCT | 365 | 0.0 | 39.452053 | 7 |