FastQCFastQC Report
Sat 18 Jun 2016
SRR3550516_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550516_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463438
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTC38160.8234111143238145No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGC34190.7377470125453675No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCG30540.6589878257717322No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19010.41019510700460476No Hit
GCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTGC14540.313742075531139Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTGC9150.1974374134188392Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTGCT7480.1614023882374773Illumina Single End Adapter 1 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCT7410.1598919380801747No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCT6350.13701940712673538No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTC5120.11047864007698979No Hit
CGCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTG5110.11026286148308943No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTCG302.1626875E-645.0000041
ATTACGG351.209828E-745.0000042
TAGCGCG302.1626875E-645.0000041
CTCGATC302.1626875E-645.00000416
CGATGAT351.209828E-745.00000410
AGGGCGC351.209828E-745.0000046
CAAAACG207.0289243E-445.01
GAGTACG207.0289243E-445.01
TTCACCG207.0289243E-445.023
ACGATAG207.0289243E-445.01
CGAGACG253.8871898E-545.015
TATCGCG253.8871898E-545.01
TATGCGG603.6379788E-1241.2500042
ACAACGA556.002665E-1140.90909213
AACGAGC556.002665E-1140.90909215
CGAATAT556.002665E-1140.90909214
CGTTTTT12750.040.7647061
ACACGAC451.9239451E-840.00000426
TACGGGA1350.040.04
TACGGCT3650.039.4520537