##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550516_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	463438
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13839175898394	33.0	31.0	34.0	30.0	34.0
2	32.24792960439152	34.0	31.0	34.0	30.0	34.0
3	32.333093099832126	34.0	31.0	34.0	30.0	34.0
4	35.876900469965776	37.0	35.0	37.0	35.0	37.0
5	35.83412236372503	37.0	35.0	37.0	35.0	37.0
6	35.84060651047174	37.0	35.0	37.0	35.0	37.0
7	36.009151170167314	37.0	35.0	37.0	35.0	37.0
8	35.96155041235289	37.0	35.0	37.0	35.0	37.0
9	37.57621299936561	39.0	38.0	39.0	35.0	39.0
10	37.29863973174405	39.0	37.0	39.0	34.0	39.0
11	37.266214682438644	39.0	37.0	39.0	34.0	39.0
12	37.18702178069127	39.0	37.0	39.0	34.0	39.0
13	37.10918396851359	39.0	37.0	39.0	33.0	39.0
14	38.31678886927701	40.0	38.0	41.0	34.0	41.0
15	38.349744733923416	40.0	38.0	41.0	34.0	41.0
16	38.41231189501077	40.0	38.0	41.0	34.0	41.0
17	38.4074396143605	40.0	38.0	41.0	34.0	41.0
18	38.34477966847777	40.0	37.0	41.0	34.0	41.0
19	38.27980441828249	40.0	37.0	41.0	34.0	41.0
20	38.1254989879984	40.0	37.0	41.0	34.0	41.0
21	38.13050073580501	40.0	37.0	41.0	34.0	41.0
22	38.118412818974704	40.0	37.0	41.0	34.0	41.0
23	38.08430469663688	40.0	37.0	41.0	34.0	41.0
24	38.05760425342764	40.0	37.0	41.0	34.0	41.0
25	38.03270340369154	40.0	37.0	41.0	34.0	41.0
26	37.8948208821892	40.0	36.0	41.0	33.0	41.0
27	37.82636728106025	40.0	36.0	41.0	33.0	41.0
28	37.75008091697271	40.0	36.0	41.0	33.0	41.0
29	37.70902688169723	40.0	36.0	41.0	33.0	41.0
30	37.66438660619112	40.0	36.0	41.0	33.0	41.0
31	37.551931434194	40.0	36.0	41.0	33.0	41.0
32	37.33706989931771	40.0	36.0	41.0	32.0	41.0
33	37.174633931615446	40.0	35.0	41.0	32.0	41.0
34	36.982485251533106	40.0	35.0	41.0	31.0	41.0
35	36.92526292621667	40.0	35.0	41.0	31.0	41.0
36	36.7862669871698	39.0	35.0	41.0	31.0	41.0
37	36.73802752471744	39.0	35.0	41.0	31.0	41.0
38	36.68220991804729	39.0	35.0	41.0	31.0	41.0
39	36.59704426482075	39.0	35.0	41.0	30.0	41.0
40	36.50123856912899	39.0	35.0	41.0	30.0	41.0
41	36.337920066977674	39.0	35.0	41.0	30.0	41.0
42	36.29573535187015	39.0	35.0	41.0	30.0	41.0
43	36.225782089513594	39.0	35.0	41.0	29.0	41.0
44	36.08139815897704	39.0	35.0	41.0	29.0	41.0
45	36.024376507752926	39.0	35.0	41.0	29.0	41.0
46	35.89981831442394	39.0	35.0	40.0	28.0	41.0
47	35.73902010624938	38.0	35.0	40.0	28.0	41.0
48	35.626297368795825	38.0	35.0	40.0	28.0	41.0
49	35.53231284443658	38.0	35.0	40.0	28.0	41.0
50	35.431790228682154	38.0	34.0	40.0	27.0	41.0
51	34.14485432787126	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	3.0
11	3.0
12	2.0
13	4.0
14	3.0
15	12.0
16	14.0
17	48.0
18	105.0
19	211.0
20	420.0
21	704.0
22	1061.0
23	1569.0
24	2331.0
25	3342.0
26	4631.0
27	5223.0
28	5670.0
29	6332.0
30	7548.0
31	9564.0
32	11968.0
33	15860.0
34	25961.0
35	35358.0
36	33850.0
37	50258.0
38	88779.0
39	152496.0
40	103.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.61273784195513	21.09322066813684	23.91733090510489	11.376710584803146
2	31.783323767149003	26.900685744371415	24.686581592359712	16.62940889611987
3	29.585618788273727	26.969950673013432	27.004043690849695	16.440386847863145
4	26.435682874516115	24.65227279592955	30.380547128202696	18.531497201351637
5	27.014401063356914	28.57426451866269	25.465973873527854	18.945360544452548
6	24.453756489541213	35.51370409849861	25.336722495781526	14.695816916178646
7	77.55147398357494	5.66354075410303	11.962765245836552	4.822220016485485
8	78.27778473064359	6.886573824330331	9.874460014068765	4.961181430957323
9	71.90174305948153	7.285332665858216	11.580837134632896	9.23208714002736
10	39.175898394175704	24.20755311390089	20.469188974576966	16.14735951734644
11	30.80865185850103	24.88445056296635	24.78001372351857	19.526883855014045
12	26.875439648885074	22.948916575680027	29.27122937696089	20.904414398474014
13	24.0972902524178	23.9822802618689	32.448784950737746	19.471644534975553
14	21.051143842326265	28.47932193734653	28.71754150501254	21.751992715314667
15	19.48221768607667	26.22314095952425	34.49501335669496	19.799627997704118
16	21.174784976631177	26.150207794785924	30.733776686417603	21.941230542165293
17	21.427677488682413	25.93162407916485	29.63373741471351	23.006961017439227
18	22.14039418433534	25.912419784307716	29.78068263715966	22.16650339419728
19	22.854621330145562	28.255127978284044	27.306349500904116	21.58390119066628
20	24.92372226705622	26.281616958471254	29.141977999214564	19.65268277525796
21	23.54489705203285	28.473280136717317	28.38783181353277	19.59399099771706
22	22.448741795018968	24.984140273348324	28.2300976613916	24.337020270241112
23	21.531682770942393	28.331082043336973	28.427750853404337	21.709484332316297
24	22.60108148231263	25.15935249159542	28.794574463034966	23.444991563056977
25	21.323672206422433	29.391418053763395	26.634199180904456	22.650710558909715
26	21.060853879051784	27.38920848096185	28.715599497667434	22.83433814231893
27	22.772409685869523	28.9272782982837	26.720510618464605	21.579801397382173
28	20.030726871771414	28.148749131491158	29.077675978232254	22.74284801850517
29	23.37054794816135	25.815966752834253	27.186592381289405	23.626892917714994
30	21.846503739443033	28.657986613096035	27.28282963416897	22.212680013291962
31	22.609065290286942	29.372861094687963	25.06311523871586	22.954958376309236
32	23.991558741406617	28.980791389570985	24.461092961733826	22.566556907288568
33	21.89721170900962	29.047682753680103	24.0416193751915	25.013486162118774
34	22.290576085689995	29.258498439920764	25.497045991049504	22.953879483339733
35	22.81189716855329	28.039780941571475	24.538557476944057	24.609764412931177
36	22.114932310255092	31.955083527893695	23.75873363858812	22.17125052326309
37	21.405668072104575	31.01299418692468	26.11136764788386	21.469970093086886
38	21.11328807736957	29.36034593624174	24.45246181797781	25.073904168410877
39	22.2344736512759	27.90772444210445	24.97723535834351	24.880566548276146
40	23.31164039202655	25.821576996275663	27.83975418502583	23.02702842667196
41	20.449768901125935	30.395220072587918	25.945002351986673	23.210008674299477
42	23.004803231500222	28.011082388582725	27.081076648872127	21.90303773104493
43	22.698397628161697	28.166227197597088	25.93486075807336	23.20051441616786
44	21.62425178772565	27.279377176666564	26.30211592489179	24.794255110715994
45	21.709484332316297	25.545164617489284	25.743033588095926	27.00231746209849
46	23.2827260604439	27.745243160897466	25.306513492635474	23.665517286023157
47	20.93613385177737	26.697638087511166	29.09903805902839	23.267190001683073
48	21.806153142383664	26.941683677212485	26.58910145477928	24.663061725624573
49	21.572680703783462	25.689088939620834	28.586995455702812	24.15123490089289
50	20.17529852968466	25.450653593360922	28.5233407705022	25.850707106452216
51	20.484509254743895	24.852731109662997	25.86732205818254	28.79543757741057
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	108.0
1	342.5
2	577.0
3	2007.5
4	3438.0
5	2394.5
6	1351.0
7	1337.5
8	1324.0
9	1323.0
10	1322.0
11	1310.5
12	1299.0
13	1306.5
14	1314.0
15	1248.5
16	1183.0
17	1182.0
18	1181.0
19	1222.0
20	1263.0
21	1393.5
22	1524.0
23	1623.0
24	1722.0
25	2077.5
26	2934.5
27	3436.0
28	3906.5
29	4377.0
30	5568.0
31	6759.0
32	7387.5
33	8016.0
34	9831.5
35	11647.0
36	12744.5
37	13842.0
38	15439.5
39	17037.0
40	18930.5
41	20824.0
42	23096.5
43	25369.0
44	28298.5
45	31228.0
46	41916.5
47	52605.0
48	50296.0
49	47987.0
50	46388.5
51	44790.0
52	39658.5
53	34527.0
54	30292.0
55	26057.0
56	23667.5
57	21278.0
58	19763.5
59	18249.0
60	17990.0
61	17731.0
62	15503.5
63	13276.0
64	10960.0
65	8644.0
66	7280.5
67	5917.0
68	4678.0
69	3439.0
70	2943.5
71	2448.0
72	2059.0
73	1670.0
74	1306.5
75	780.5
76	618.0
77	499.0
78	380.0
79	257.5
80	135.0
81	118.5
82	102.0
83	65.0
84	28.0
85	22.5
86	17.0
87	11.5
88	6.0
89	6.5
90	7.0
91	6.0
92	5.0
93	5.0
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	463438.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.19104474363691
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.71980299104396	28.89156097514102
2	9.32626973265753	7.310125087676157
3	3.5950356629029567	4.226796105594004
4	2.1786253362303483	3.4153041212729836
5	1.5730532269996993	3.082479970173382
6	1.3199904118158021	3.103908197438688
7	1.0525582752260447	2.8875600921768445
8	0.8502342820598859	2.665725583262639
9	0.7929366137974662	2.796841287918399
>10	5.516849586870592	34.97022045718783
>50	0.04644508113511722	1.2444747720639362
>100	0.022116705302436625	1.5682159020419049
>500	0.0033175057953654945	0.8802118272895146
>1k	0.002764588162804578	2.9565756207627127
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTC	3816	0.8234111143238145	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGC	3419	0.7377470125453675	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCG	3054	0.6589878257717322	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1901	0.41019510700460476	No Hit
GCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTGC	1454	0.313742075531139	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTGC	915	0.1974374134188392	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTGCT	748	0.1614023882374773	Illumina Single End Adapter 1 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCT	741	0.1598919380801747	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCT	635	0.13701940712673538	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTC	512	0.11047864007698979	No Hit
CGCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTG	511	0.11026286148308943	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.18513803356651806	0.0
2	0.0	0.0	0.0	0.885339570773221	0.0
3	0.0	0.0	0.0	1.2681307963524786	0.0
4	0.0	0.0	0.0	1.8421018561274647	0.0
5	0.0	0.0	0.0	3.836974956736392	0.0
6	0.0	0.0	0.0	4.824809359612289	0.0
7	0.0	0.0	0.0	5.736258140247455	0.0
8	0.0	0.0	0.0	7.110552004798916	0.0
9	0.0	0.0	0.0	7.617415921870887	0.0
10	0.0	0.0	0.0	9.346449794794557	0.0
11	0.0	0.0	0.0	10.695929121047476	0.0
12	0.0	0.0	0.0	12.539109870144442	0.0
13	0.0	0.0	0.0	13.070140989733254	0.0
14	0.0	0.0	0.0	13.315697029591876	0.0
15	0.0	0.0	0.0	13.752001346458426	0.0
16	0.0	0.0	0.0	14.450260876320026	0.0
17	0.0	0.0	0.0	15.26201994657322	0.0
18	0.0	0.0	0.0	16.119524078733292	0.0
19	0.0	0.0	0.0	16.772901661063617	0.0
20	0.0	0.0	0.0	17.327668425981468	0.0
21	0.0	0.0	0.0	18.02808574178207	0.0
22	0.0	0.0	0.0	18.78244770605777	0.0
23	0.0	0.0	0.0	19.549540607373586	0.0
24	0.0	0.0	0.0	20.1403423974728	0.0
25	0.0	0.0	0.0	20.67590486753352	0.0
26	0.0	0.0	0.0	21.17111674053487	0.0
27	0.0	0.0	0.0	21.68423823682995	0.0
28	0.0	0.0	0.0	22.221526935641876	0.0
29	0.0	0.0	0.0	22.74694781178928	0.0
30	0.0	0.0	0.0	23.373353069882054	0.0
31	0.0	0.0	0.0	23.92768827761211	0.0
32	0.0	0.0	0.0	24.46972410548984	0.0
33	0.0	0.0	0.0	24.98867162382023	0.0
34	0.0	0.0	0.0	25.49985111277021	0.0
35	0.0	0.0	0.0	26.03519780423703	0.0
36	2.1577859390037072E-4	0.0	0.0	26.572270724455052	0.0
37	2.1577859390037072E-4	0.0	0.0	27.12962683249971	0.0
38	6.473357817011121E-4	0.0	0.0	27.6485743508301	0.0
39	6.473357817011121E-4	0.0	0.0	28.190825957301733	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTTTCG	30	2.1626875E-6	45.000004	1
ATTACGG	35	1.209828E-7	45.000004	2
TAGCGCG	30	2.1626875E-6	45.000004	1
CTCGATC	30	2.1626875E-6	45.000004	16
CGATGAT	35	1.209828E-7	45.000004	10
AGGGCGC	35	1.209828E-7	45.000004	6
CAAAACG	20	7.0289243E-4	45.0	1
GAGTACG	20	7.0289243E-4	45.0	1
TTCACCG	20	7.0289243E-4	45.0	23
ACGATAG	20	7.0289243E-4	45.0	1
CGAGACG	25	3.8871898E-5	45.0	15
TATCGCG	25	3.8871898E-5	45.0	1
TATGCGG	60	3.6379788E-12	41.250004	2
ACAACGA	55	6.002665E-11	40.909092	13
AACGAGC	55	6.002665E-11	40.909092	15
CGAATAT	55	6.002665E-11	40.909092	14
CGTTTTT	1275	0.0	40.764706	1
ACACGAC	45	1.9239451E-8	40.000004	26
TACGGGA	135	0.0	40.0	4
TACGGCT	365	0.0	39.452053	7
>>END_MODULE