FastQCFastQC Report
Sat 18 Jun 2016
SRR3550513_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550513_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences582958
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31660.5430922982444704No Hit
GCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGC15020.2576514946188233No Hit
GAATCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTC12450.21356598588577563No Hit
CCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGC11300.19383900726982045No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGC9160.15712967314969517No Hit
CTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGCT8870.15215504375958475Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCG8310.142548862868337No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT6760.11596032647291914No Hit
CGCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTG6650.11407339808356691No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCACGT302.1636533E-645.00000427
TTGCGTG302.1636533E-645.0000041
TGTTACG351.2105556E-745.0000041
TTAACGG302.1636533E-645.0000042
GATCGCC207.0304226E-445.09
CAGTTAG207.0304226E-445.01
TAGCGTA207.0304226E-445.01
CCAGACG207.0304226E-445.021
TCAGGCG253.888432E-545.019
TCCCGTA253.888432E-545.044
ACGTAAG253.888432E-545.01
TCGAGAG207.0304226E-445.01
CGTTTTT16150.042.4922641
CGGGTTA451.9255822E-840.06
TTTAGCG451.9255822E-840.01
TATAGCG451.9255822E-840.01
TGATTCG403.4543154E-739.37515
TAGTGCG356.2429917E-638.571431
TAACGGG1300.038.076923
TTACGGG1900.037.8947373