##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550513_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	582958
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.204959877040885	33.0	31.0	34.0	30.0	34.0
2	32.32816600852892	34.0	31.0	34.0	30.0	34.0
3	32.409305644660506	34.0	31.0	34.0	30.0	34.0
4	35.92652472390807	37.0	35.0	37.0	35.0	37.0
5	35.891551363906146	37.0	35.0	37.0	35.0	37.0
6	35.9152048689614	37.0	35.0	37.0	35.0	37.0
7	36.09059829353058	37.0	35.0	37.0	35.0	37.0
8	36.082204892976854	37.0	35.0	37.0	35.0	37.0
9	37.6676518720045	39.0	38.0	39.0	35.0	39.0
10	37.444354481798	39.0	37.0	39.0	35.0	39.0
11	37.38174791322874	39.0	37.0	39.0	35.0	39.0
12	37.30948884825322	39.0	37.0	39.0	34.0	39.0
13	37.25004031165196	39.0	37.0	39.0	34.0	39.0
14	38.46755169326092	40.0	38.0	41.0	34.0	41.0
15	38.51879380675795	40.0	38.0	41.0	34.0	41.0
16	38.54085886118725	40.0	38.0	41.0	34.0	41.0
17	38.54401689315525	40.0	38.0	41.0	34.0	41.0
18	38.488235859187114	40.0	38.0	41.0	34.0	41.0
19	38.432487417618425	40.0	37.0	41.0	34.0	41.0
20	38.29724782917466	40.0	37.0	41.0	34.0	41.0
21	38.25764806383993	40.0	37.0	41.0	34.0	41.0
22	38.237214344772696	40.0	37.0	41.0	34.0	41.0
23	38.20268870141588	40.0	37.0	41.0	34.0	41.0
24	38.18049670816765	40.0	37.0	41.0	34.0	41.0
25	38.13783497267384	40.0	37.0	41.0	34.0	41.0
26	38.00729726669846	40.0	37.0	41.0	34.0	41.0
27	37.92999324136559	40.0	36.0	41.0	33.0	41.0
28	37.861046593408105	40.0	36.0	41.0	33.0	41.0
29	37.809720425828274	40.0	36.0	41.0	33.0	41.0
30	37.761620905794246	40.0	36.0	41.0	33.0	41.0
31	37.646708682272134	40.0	36.0	41.0	33.0	41.0
32	37.46872673503066	40.0	36.0	41.0	33.0	41.0
33	37.30728285742712	40.0	36.0	41.0	32.0	41.0
34	37.1159174417368	40.0	35.0	41.0	31.0	41.0
35	37.0540896599755	40.0	35.0	41.0	31.0	41.0
36	36.85699312814988	39.0	35.0	41.0	31.0	41.0
37	36.838624737974264	39.0	35.0	41.0	31.0	41.0
38	36.76680309730718	39.0	35.0	41.0	31.0	41.0
39	36.66122602314403	39.0	35.0	41.0	30.0	41.0
40	36.591222695288515	39.0	35.0	41.0	30.0	41.0
41	36.417330236483586	39.0	35.0	41.0	30.0	41.0
42	36.31115792218307	39.0	35.0	41.0	30.0	41.0
43	36.226757330716794	39.0	35.0	41.0	29.0	41.0
44	36.0664198793052	39.0	35.0	41.0	29.0	41.0
45	36.014790087793635	39.0	35.0	41.0	29.0	41.0
46	35.879763207641034	38.0	35.0	40.0	28.0	41.0
47	35.75789679530944	38.0	35.0	40.0	28.0	41.0
48	35.61956264430714	38.0	35.0	40.0	28.0	41.0
49	35.519323862096414	38.0	35.0	40.0	28.0	41.0
50	35.430881813098026	38.0	34.0	40.0	27.0	41.0
51	34.121025871503605	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	6.0
11	7.0
12	4.0
13	9.0
14	8.0
15	13.0
16	18.0
17	47.0
18	117.0
19	222.0
20	400.0
21	688.0
22	1065.0
23	1666.0
24	2474.0
25	3827.0
26	5420.0
27	6407.0
28	7086.0
29	8063.0
30	9285.0
31	11387.0
32	14274.0
33	19300.0
34	31978.0
35	43876.0
36	43523.0
37	64094.0
38	114060.0
39	193494.0
40	138.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.65661677170568	22.237622607460573	25.334415172276564	11.771345448557186
2	32.47335142497401	25.661539939412442	24.485297397068056	17.379811238545486
3	30.236483588869177	25.036280486758912	28.355215984685	16.372019939686908
4	27.79582748671431	25.762576377715035	27.787593617378953	18.654002518191707
5	25.456207822862027	29.370898074989967	25.88522672302293	19.287667379125082
6	25.89208828080239	33.96574024200714	24.97281107729888	15.169360399891588
7	79.77710229553415	5.815341757039101	9.687661889878859	4.719894057547886
8	80.51283282843704	5.360935093094185	9.244233718381084	4.881998360087691
9	73.4172959286947	8.025106439915055	11.400306711632743	7.1572909197575125
10	37.98781387338367	31.041858933233613	17.395935899327224	13.574391294055491
11	27.890860061959867	24.796297503422203	27.8687315381211	19.444110896496834
12	26.796784674024543	22.595281306715066	30.503398186490276	20.104535832770114
13	23.27354629321495	23.810634728402388	32.4390436360767	20.476775342305963
14	20.15170904250392	27.14964028283341	30.584879185121398	22.11377148954127
15	19.63760682587768	25.75828790410287	33.83399833264146	20.770106937377992
16	22.426315446395794	26.01096476933158	31.03791353751042	20.524806246762203
17	22.124063826210463	26.503453078952514	28.625904439084803	22.746578655752213
18	23.071301877665288	26.060711063232684	29.549813194089452	21.318173865012575
19	23.144377468016565	27.413981796287214	27.745051959146284	21.69658877654994
20	25.862926660239676	26.448560616716815	27.93151479180318	19.75699793124033
21	24.239653628563293	27.70388261246951	28.00064498643127	20.05581877253593
22	23.23237694653817	24.954971027072276	27.85672381200704	23.95592821438251
23	22.66698458551045	26.83383708603364	28.419886166756434	22.07929216169947
24	23.497233076825434	24.507940537740282	29.421158985724528	22.573667399709755
25	21.855262300200014	27.749168893813962	27.31826306526371	23.077305740722316
26	22.253061112464362	27.644187059788184	27.465958096466643	22.636793731280815
27	22.993423196868385	27.62771932111747	27.26371368091698	22.11514380109716
28	20.193393006014155	28.480610953104684	28.595370506966194	22.730625533914964
29	22.954483856469935	25.848002772069346	27.686214101187396	23.51129927027333
30	23.75111071466555	26.892331866103564	27.728927298364546	21.62763012086634
31	24.469515814175292	28.41456845947736	25.20507480813369	21.910840918213662
32	25.13628769139458	27.798229031937122	25.111757622333	21.953725654335305
33	23.37578350412894	27.770096645041324	26.494533053839213	22.35958679699052
34	23.4018574236909	26.48458379505899	27.654479396457376	22.45907938479273
35	23.808576261068552	26.593511024807963	26.316475629462154	23.28143708466133
36	23.52176314588701	28.739634759279397	27.00897834835443	20.729623746479163
37	23.580772542790392	28.10099526895591	27.649504767067267	20.668727421186432
38	22.363532192713713	27.616740828670334	27.16576494361515	22.853962035000805
39	23.973082108831168	24.671074073947008	28.36876756129944	22.987076255922382
40	25.613337496011717	23.61576648746565	29.611567214104618	21.159328802418013
41	23.238380809595203	25.47044555525441	27.75568737370445	23.535486261445936
42	23.265655501768567	26.227961534107084	27.763921243039807	22.742461721084535
43	23.227230778203577	25.56427735788856	28.15520157541367	23.053290288494196
44	22.817081161936194	25.877335931576546	27.261140596749676	24.04444230973758
45	23.150724408962567	24.736773489685362	26.675678179217027	25.436823922135044
46	23.79862700228833	25.88316825568909	26.764363813516585	23.553840928506
47	22.799069572765106	25.011578878752843	29.574343263151032	22.61500828533102
48	22.82668734282744	24.34617931308945	28.800016467738672	24.027116876344436
49	22.554798115816233	24.060223892630344	29.777273834478642	23.607704157074778
50	21.404972570922776	23.934828924210663	29.933545812905905	24.726652691960656
51	21.422641082204894	23.105438127618115	28.211123271316286	27.26079751886071
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	176.0
1	436.5
2	697.0
3	2871.0
4	5045.0
5	3333.0
6	1621.0
7	1572.0
8	1523.0
9	1522.0
10	1521.0
11	1530.5
12	1540.0
13	1607.0
14	1674.0
15	1688.0
16	1702.0
17	1669.5
18	1637.0
19	1618.5
20	1600.0
21	1778.5
22	1957.0
23	2013.0
24	2069.0
25	2579.0
26	3546.0
27	4003.0
28	5292.0
29	6581.0
30	7362.0
31	8143.0
32	9448.5
33	10754.0
34	12778.0
35	14802.0
36	16002.0
37	17202.0
38	18855.0
39	20508.0
40	22916.5
41	25325.0
42	28231.0
43	31137.0
44	35200.0
45	39263.0
46	46637.0
47	54011.0
48	57272.0
49	60533.0
50	58569.5
51	56606.0
52	49445.0
53	42284.0
54	38195.5
55	34107.0
56	31392.0
57	28677.0
58	27192.0
59	25707.0
60	24535.5
61	23364.0
62	20969.5
63	18575.0
64	15697.5
65	12820.0
66	10748.5
67	8677.0
68	7006.0
69	5335.0
70	4354.0
71	3373.0
72	2770.5
73	2168.0
74	1827.5
75	1182.5
76	878.0
77	635.0
78	392.0
79	305.0
80	218.0
81	166.5
82	115.0
83	71.0
84	27.0
85	21.0
86	15.0
87	13.5
88	12.0
89	9.0
90	6.0
91	4.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	582958.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.938475294375664
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.53685495608153	26.428024450652227
2	8.945982084529284	6.430099122575659
3	3.4448115158005113	3.714038206631324
4	2.0160655126526508	2.898172824732404
5	1.3882054225627145	2.4944993141144223
6	1.1674436732122218	2.517368736438757
7	1.0010461254549274	2.5183250013734675
8	0.8697833766891104	2.5006950715680194
9	0.7561763402670397	2.4458242250590576
>10	6.773037089346953	42.49020622145016
>50	0.06083536062987999	1.4460766048911993
>100	0.0344893383092875	2.0424110524053654
>500	0.0033531301134029517	0.8617927957131878
>1k	0.0019160743505159724	1.2124663723947227
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3166	0.5430922982444704	No Hit
GCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGC	1502	0.2576514946188233	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTC	1245	0.21356598588577563	No Hit
CCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGC	1130	0.19383900726982045	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGC	916	0.15712967314969517	No Hit
CTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGCT	887	0.15215504375958475	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCG	831	0.142548862868337	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	676	0.11596032647291914	No Hit
CGCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTG	665	0.11407339808356691	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.17376895076489215	0.0
2	0.0	0.0	0.0	0.8520339372647773	0.0
3	0.0	0.0	0.0	1.2764212859245434	0.0
4	0.0	0.0	0.0	1.7601611093766618	0.0
5	0.0	0.0	0.0	3.274335372359587	0.0
6	0.0	0.0	0.0	4.327241413618133	0.0
7	0.0	0.0	0.0	5.257668648513272	0.0
8	0.0	0.0	0.0	6.716607371371523	0.0
9	0.0	0.0	0.0	7.379433852867616	0.0
10	0.0	0.0	0.0	8.62789429084085	0.0
11	0.0	0.0	0.0	10.062302944637521	0.0
12	0.0	0.0	0.0	11.335808068505793	0.0
13	0.0	0.0	0.0	11.830183306516078	0.0
14	0.0	0.0	0.0	12.050267772292344	0.0
15	0.0	0.0	0.0	12.38819949293088	0.0
16	0.0	0.0	0.0	13.017918958141067	0.0
17	0.0	0.0	0.0	13.817805056281928	0.0
18	0.0	0.0	0.0	14.628669646869929	0.0
19	0.0	0.0	0.0	15.190802767952409	0.0
20	0.0	0.0	0.0	15.733551988307905	0.0
21	0.0	0.0	0.0	16.389002295191077	0.0
22	0.0	0.0	0.0	17.099688142198925	0.0
23	0.0	0.0	0.0	17.76234308475053	0.0
24	0.0	0.0	0.0	18.298745364160027	0.0
25	0.0	0.0	0.0	18.775966707721654	0.0
26	0.0	0.0	0.0	19.225055664387487	0.0
27	0.0	0.0	0.0	19.67465923788678	0.0
28	0.0	0.0	0.0	20.126835895553366	0.0
29	0.0	0.0	0.0	20.62344113984198	0.0
30	0.0	0.0	0.0	21.20598739531836	0.0
31	1.7153894448656678E-4	0.0	0.0	21.694530309216102	0.0
32	1.7153894448656678E-4	0.0	0.0	22.17175165277773	0.0
33	1.7153894448656678E-4	0.0	0.0	22.64897299633936	0.0
34	1.7153894448656678E-4	0.0	0.0	23.12053355473293	0.0
35	1.7153894448656678E-4	0.0	0.0	23.61267878646489	0.0
36	1.7153894448656678E-4	0.0	0.0	24.11048480336491	0.0
37	1.7153894448656678E-4	0.0	0.0	24.600228489874056	0.0
38	1.7153894448656678E-4	0.0	0.0	25.089286020605257	0.0
39	1.7153894448656678E-4	0.0	0.0	25.580573557614784	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCACGT	30	2.1636533E-6	45.000004	27
TTGCGTG	30	2.1636533E-6	45.000004	1
TGTTACG	35	1.2105556E-7	45.000004	1
TTAACGG	30	2.1636533E-6	45.000004	2
GATCGCC	20	7.0304226E-4	45.0	9
CAGTTAG	20	7.0304226E-4	45.0	1
TAGCGTA	20	7.0304226E-4	45.0	1
CCAGACG	20	7.0304226E-4	45.0	21
TCAGGCG	25	3.888432E-5	45.0	19
TCCCGTA	25	3.888432E-5	45.0	44
ACGTAAG	25	3.888432E-5	45.0	1
TCGAGAG	20	7.0304226E-4	45.0	1
CGTTTTT	1615	0.0	42.492264	1
CGGGTTA	45	1.9255822E-8	40.0	6
TTTAGCG	45	1.9255822E-8	40.0	1
TATAGCG	45	1.9255822E-8	40.0	1
TGATTCG	40	3.4543154E-7	39.375	15
TAGTGCG	35	6.2429917E-6	38.57143	1
TAACGGG	130	0.0	38.07692	3
TTACGGG	190	0.0	37.894737	3
>>END_MODULE