FastQCFastQC Report
Sat 18 Jun 2016
SRR3550512_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550512_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399319
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT9740.24391526573992223No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8070.2020940651459109No Hit
GCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTGC6740.16878736048121928TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTGC5540.1387361983777381TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTGCT4590.1149456950458155TruSeq Adapter, Index 21 (95% over 23bp)
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4130.10342608290614771No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGA207.0277514E-445.024
CCGATTT207.0277514E-445.031
CGGTCTA207.0277514E-445.031
ATAACGG502.1827873E-1145.02
AATAGCG253.8862177E-545.01
GCGTAAG207.0277514E-445.01
TTGGACG302.1619308E-644.9999961
GCTACGA1850.043.78378710
CGAATAT1850.041.35135314
CTACGAA2000.040.511
TACGAAT1900.040.26315712
ATAGGGA2750.040.0909084
TTGTCGG451.9228537E-840.0000042
TGAACGG451.9228537E-840.0000042
CATACGG356.2380514E-638.571432
CGTTTGG356.2380514E-638.571432
AGCTACG2050.038.4146359
ACACGAC301.1385884E-437.49999626
CACGACC301.1385884E-437.49999627
CGAGGGT301.1385884E-437.4999964