Basic Statistics
Measure | Value |
---|---|
Filename | SRR3550512_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 399319 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 49 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT | 974 | 0.24391526573992223 | No Hit |
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 807 | 0.2020940651459109 | No Hit |
GCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTGC | 674 | 0.16878736048121928 | TruSeq Adapter, Index 14 (95% over 21bp) |
CCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTGC | 554 | 0.1387361983777381 | TruSeq Adapter, Index 14 (95% over 21bp) |
CTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTGCT | 459 | 0.1149456950458155 | TruSeq Adapter, Index 21 (95% over 23bp) |
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG | 413 | 0.10342608290614771 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTCACGA | 20 | 7.0277514E-4 | 45.0 | 24 |
CCGATTT | 20 | 7.0277514E-4 | 45.0 | 31 |
CGGTCTA | 20 | 7.0277514E-4 | 45.0 | 31 |
ATAACGG | 50 | 2.1827873E-11 | 45.0 | 2 |
AATAGCG | 25 | 3.8862177E-5 | 45.0 | 1 |
GCGTAAG | 20 | 7.0277514E-4 | 45.0 | 1 |
TTGGACG | 30 | 2.1619308E-6 | 44.999996 | 1 |
GCTACGA | 185 | 0.0 | 43.783787 | 10 |
CGAATAT | 185 | 0.0 | 41.351353 | 14 |
CTACGAA | 200 | 0.0 | 40.5 | 11 |
TACGAAT | 190 | 0.0 | 40.263157 | 12 |
ATAGGGA | 275 | 0.0 | 40.090908 | 4 |
TTGTCGG | 45 | 1.9228537E-8 | 40.000004 | 2 |
TGAACGG | 45 | 1.9228537E-8 | 40.000004 | 2 |
CATACGG | 35 | 6.2380514E-6 | 38.57143 | 2 |
CGTTTGG | 35 | 6.2380514E-6 | 38.57143 | 2 |
AGCTACG | 205 | 0.0 | 38.414635 | 9 |
ACACGAC | 30 | 1.1385884E-4 | 37.499996 | 26 |
CACGACC | 30 | 1.1385884E-4 | 37.499996 | 27 |
CGAGGGT | 30 | 1.1385884E-4 | 37.499996 | 4 |