##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550512_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	399319
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.342538171236534	33.0	31.0	34.0	30.0	34.0
2	32.44657529443878	34.0	31.0	34.0	31.0	34.0
3	32.54444441661929	34.0	31.0	34.0	31.0	34.0
4	36.03212218802511	37.0	35.0	37.0	35.0	37.0
5	35.98965989597289	37.0	35.0	37.0	35.0	37.0
6	35.9996819585344	37.0	35.0	37.0	35.0	37.0
7	36.13509249497269	37.0	35.0	37.0	35.0	37.0
8	36.110215141278026	37.0	36.0	37.0	35.0	37.0
9	37.65269871957007	39.0	38.0	39.0	35.0	39.0
10	37.47638354298193	39.0	37.0	39.0	35.0	39.0
11	37.40698539263096	39.0	37.0	39.0	35.0	39.0
12	37.285185528362035	39.0	37.0	39.0	34.0	39.0
13	37.216493580320495	39.0	37.0	39.0	34.0	39.0
14	38.44313944490495	40.0	38.0	41.0	34.0	41.0
15	38.510391441429036	40.0	38.0	41.0	34.0	41.0
16	38.55092044205259	40.0	38.0	41.0	34.0	41.0
17	38.581159423919225	40.0	38.0	41.0	34.0	41.0
18	38.52018060748424	40.0	38.0	41.0	34.0	41.0
19	38.491318970547354	40.0	37.0	41.0	34.0	41.0
20	38.3845196446951	40.0	37.0	41.0	34.0	41.0
21	38.330162100976914	40.0	37.0	41.0	34.0	41.0
22	38.30420040118302	40.0	37.0	41.0	34.0	41.0
23	38.27925793663712	40.0	37.0	41.0	34.0	41.0
24	38.24536773857493	40.0	37.0	41.0	34.0	41.0
25	38.202402089557474	40.0	37.0	41.0	34.0	41.0
26	38.076164670351275	40.0	37.0	41.0	34.0	41.0
27	38.012037994685954	40.0	37.0	41.0	34.0	41.0
28	37.92891147178071	40.0	36.0	41.0	33.0	41.0
29	37.903152617331	40.0	36.0	41.0	33.0	41.0
30	37.891202271867854	40.0	36.0	41.0	33.0	41.0
31	37.791289670664305	40.0	36.0	41.0	33.0	41.0
32	37.62860770461711	40.0	36.0	41.0	33.0	41.0
33	37.548411170016955	40.0	36.0	41.0	33.0	41.0
34	37.368031573754315	40.0	36.0	41.0	32.0	41.0
35	37.3294909583566	40.0	35.0	41.0	32.0	41.0
36	37.2059255883141	40.0	35.0	41.0	32.0	41.0
37	37.16437484817902	40.0	35.0	41.0	32.0	41.0
38	37.067560020935645	39.0	35.0	41.0	32.0	41.0
39	37.01615500389413	39.0	35.0	41.0	31.0	41.0
40	36.905797119596116	39.0	35.0	41.0	31.0	41.0
41	36.776173936126256	39.0	35.0	41.0	31.0	41.0
42	36.701617002947515	39.0	35.0	41.0	31.0	41.0
43	36.66675515064397	39.0	35.0	41.0	31.0	41.0
44	36.493447594529684	39.0	35.0	41.0	31.0	41.0
45	36.46628134398814	39.0	35.0	41.0	31.0	41.0
46	36.3424129580611	39.0	35.0	41.0	30.0	41.0
47	36.234464175258374	38.0	35.0	41.0	30.0	41.0
48	36.11188548503828	38.0	35.0	40.0	30.0	41.0
49	36.043291203273576	38.0	35.0	40.0	30.0	41.0
50	35.964732457008054	38.0	35.0	40.0	30.0	41.0
51	34.72846771628698	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	3.0
11	3.0
12	5.0
13	5.0
14	7.0
15	10.0
16	20.0
17	46.0
18	78.0
19	143.0
20	252.0
21	412.0
22	611.0
23	972.0
24	1431.0
25	1973.0
26	2754.0
27	3542.0
28	3953.0
29	4585.0
30	5611.0
31	7220.0
32	9207.0
33	12876.0
34	23109.0
35	31307.0
36	28968.0
37	42976.0
38	75963.0
39	141156.0
40	116.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.42903292856088	21.95663116455766	25.384467055161412	11.229868851720054
2	31.494870016202587	26.002018436387953	24.717080830113268	17.786030717296196
3	32.1119205447274	24.486438160969048	26.21262699746318	17.189014296840373
4	29.284857469842407	26.42874493825738	25.446823216526138	18.839574375374074
5	25.67721545931949	31.108211730471126	23.558107678322344	19.65646513188704
6	27.987648972375467	34.66075994380432	22.432942083897835	14.918648999922368
7	81.25533721160275	6.315001289695707	7.6452660654764735	4.7843954332250656
8	81.98758386152424	6.137449006934306	7.538584440009116	4.336382691532334
9	74.71420092707835	8.883373944139898	9.83799919362715	6.564425935154601
10	40.912904219433585	27.99516176290134	16.258680403386766	14.833253614278307
11	31.770589428502028	25.256499189870755	23.464949075801552	19.507962305825668
12	29.47217638028744	23.29265574640826	27.1442130226711	20.090954850633203
13	25.55550825280039	25.017842877498943	28.388831986456943	21.037816883243725
14	20.924373746303083	27.59247619071469	28.3432543906	23.13989567238223
15	20.636133016460523	26.3022796310719	31.74204082450372	21.31954652796386
16	22.877448856678495	25.515189610311555	30.138811326282998	21.468550206726952
17	22.828365291909474	25.93265033719908	27.683881808779446	23.555102562111998
18	23.81955278862263	25.90936068656888	28.24734109821972	22.023745426588768
19	25.612104608095283	26.620321096667077	25.681472707284154	22.08610158795349
20	27.36058138981616	25.422531860492487	26.822916014514714	20.39397073517664
21	25.253494073660406	26.872500431985458	26.567981989336847	21.30602350501729
22	24.54403622166739	25.201906245382766	26.142758045572588	24.11129948737726
23	24.49395095149492	26.096178744312194	26.77984268216639	22.6300276220265
24	23.653520118000895	24.760655015163316	28.55361252532436	23.032212341511425
25	23.072030131298536	27.10639864369088	26.19735099006058	23.624220234950002
26	23.16443745476674	28.183732805100686	25.76236041861269	22.889469321519886
27	23.116105169050307	27.63379653860698	26.46305334832552	22.78704494401719
28	21.17329753906025	28.546099734798496	27.066831280254632	23.21377144588662
29	22.322754489518402	26.91582419068464	27.404656427567936	23.35676489222902
30	23.155422106135696	26.495107921235906	26.282495949353773	24.066974023274625
31	24.069227860432385	27.98890110412978	24.743626023304678	23.198245012133157
32	23.98383247478833	28.29792722109391	25.761108286858374	21.957132017259383
33	24.567325872297587	27.908514245502968	24.76666524758401	22.757494634615433
34	23.299667684232407	27.79431982950974	26.272228468968418	22.633784017289436
35	23.44416368867998	26.251443081846844	26.449279899028095	23.855113330445082
36	23.596673336355146	29.40280828109857	24.59762746075193	22.402890921794356
37	23.646758606527612	28.761466396540108	26.455540557799655	21.136234439132622
38	23.564618763444763	27.15848732467025	26.572740090003233	22.704153821881754
39	25.280540119553542	25.514438331258972	26.657133770243842	22.54788777894365
40	25.060916209847267	25.148815858999946	28.19525241724035	21.595015513912436
41	22.224086507278642	26.511385634041957	27.184531665159934	24.079996193519467
42	22.73270242588006	26.430998775415144	27.38537359855153	23.45092520015326
43	23.848101392620936	25.83999258738001	27.029768180327007	23.282137839672043
44	23.008171411828638	26.311545406053803	26.203110796130414	24.477172385987142
45	23.056754123895935	25.4450702320701	26.34359997896419	25.154575665069785
46	23.269366095778064	26.892284113703578	25.998011614774153	23.840338175744204
47	22.91025470864146	26.35662214920903	28.040238506056564	22.69288463609295
48	22.441707006178017	25.230204423030212	28.37080128919485	23.95728728159692
49	22.828365291909474	25.69699914103762	27.992657499392713	23.481978067660194
50	21.28548854424658	25.522451974486565	28.543845897640736	24.648213583626124
51	21.76330202169193	24.77718315432023	27.16925565775733	26.290259166230506
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	142.0
1	404.0
2	666.0
3	1153.0
4	1640.0
5	1204.0
6	768.0
7	732.5
8	697.0
9	706.5
10	716.0
11	761.0
12	806.0
13	794.5
14	783.0
15	752.5
16	722.0
17	758.5
18	795.0
19	785.5
20	776.0
21	878.0
22	980.0
23	1181.5
24	1383.0
25	1709.5
26	2431.5
27	2827.0
28	3661.0
29	4495.0
30	5414.0
31	6333.0
32	7394.5
33	8456.0
34	9593.5
35	10731.0
36	11702.0
37	12673.0
38	13620.0
39	14567.0
40	16468.0
41	18369.0
42	19857.5
43	21346.0
44	23286.5
45	25227.0
46	28261.5
47	31296.0
48	33435.5
49	35575.0
50	34854.5
51	34134.0
52	31036.5
53	27939.0
54	25323.0
55	22707.0
56	21395.5
57	20084.0
58	20040.0
59	19996.0
60	19574.5
61	19153.0
62	17771.5
63	16390.0
64	14034.5
65	11679.0
66	9782.5
67	7886.0
68	6759.5
69	5633.0
70	4515.5
71	3398.0
72	2821.5
73	2245.0
74	1816.0
75	1199.0
76	1011.0
77	746.0
78	481.0
79	362.0
80	243.0
81	174.0
82	105.0
83	64.0
84	23.0
85	16.0
86	9.0
87	7.0
88	5.0
89	4.0
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	399319.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.90266613175914
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.67611359145657	24.66784796172893
2	8.25547905697244	5.762764585664778
3	3.365070116562035	3.5234975636497365
4	2.257065928649272	3.1511047418005775
5	1.8717983581355364	3.2665376579989776
6	1.541836987260079	3.2288532957561547
7	1.4296089273109607	3.4928014162241747
8	1.321407779563974	3.6896523643224453
9	1.1915555780944405	3.7429619867698953
>10	8.010207170140285	41.91037366709815
>50	0.057554238453618324	1.3843117979348003
>100	0.019424555478096184	1.4237344266806309
>500	0.0028777119226809163	0.7555585343707263
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	974	0.24391526573992223	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	807	0.2020940651459109	No Hit
GCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTGC	674	0.16878736048121928	TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTGC	554	0.1387361983777381	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTGCT	459	0.1149456950458155	TruSeq Adapter, Index 21 (95% over 23bp)
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	413	0.10342608290614771	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.008527017246863E-4	0.0	0.0	0.13147383420273015	0.0
2	5.008527017246863E-4	0.0	0.0	0.6002719630170366	0.0
3	7.512790525870294E-4	0.0	0.0	0.8594632361595617	0.0
4	7.512790525870294E-4	0.0	0.0	1.1594740044926488	0.0
5	7.512790525870294E-4	0.0	0.0	2.0642644101582945	0.0
6	7.512790525870294E-4	0.0	0.0	2.6773081170693107	0.0
7	7.512790525870294E-4	0.0	0.0	3.2610519409294323	0.0
8	7.512790525870294E-4	0.0	0.0	4.17460726887526	0.0
9	7.512790525870294E-4	0.0	0.0	4.53046311345065	0.0
10	7.512790525870294E-4	0.0	0.0	5.281491739686817	0.0
11	7.512790525870294E-4	0.0	0.0	6.310994468081909	0.0
12	7.512790525870294E-4	0.0	0.0	7.1243792556828	0.0
13	7.512790525870294E-4	0.0	0.0	7.461703550294376	0.0
14	7.512790525870294E-4	0.0	0.0	7.600439748672114	0.0
15	7.512790525870294E-4	0.0	0.0	7.903706059566412	0.0
16	7.512790525870294E-4	0.0	0.0	8.454644031463566	0.0
17	7.512790525870294E-4	0.0	0.0	9.111512349775493	0.0
18	7.512790525870294E-4	0.0	0.0	9.754356792439127	0.0
19	7.512790525870294E-4	0.0	0.0	10.174822635537002	0.0
20	7.512790525870294E-4	0.0	0.0	10.60630723807282	0.0
21	7.512790525870294E-4	0.0	0.0	11.157245209969973	0.0
22	0.0010017054034493725	0.0	0.0	11.7349788014094	0.0
23	0.0010017054034493725	0.0	0.0	12.307703865831579	0.0
24	0.0010017054034493725	0.0	0.0	12.765483235207942	0.0
25	0.0010017054034493725	0.0	0.0	13.17467989251701	0.0
26	0.0010017054034493725	0.0	0.0	13.530034884390675	0.0
27	0.0010017054034493725	0.0	0.0	13.882635186404855	0.0
28	0.0010017054034493725	0.0	0.0	14.259276418101818	0.0
29	0.0010017054034493725	0.0	0.0	14.64217830857034	0.0
30	0.0010017054034493725	0.0	0.0	15.125751592085525	0.0
31	0.0010017054034493725	0.0	0.0	15.55047468314806	0.0
32	0.0012521317543117156	0.0	0.0	15.942892774949351	0.0
33	0.0012521317543117156	0.0	0.0	16.35334156401273	0.0
34	0.0012521317543117156	0.0	0.0	16.73899814434074	0.0
35	0.0012521317543117156	0.0	0.0	17.169981894174832	0.0
36	0.0012521317543117156	0.0	0.0	17.608478434534796	0.0
37	0.0012521317543117156	0.0	0.0	18.03745877356199	0.0
38	0.0012521317543117156	0.0	0.0	18.4403947720995	0.0
39	0.0012521317543117156	0.0	0.0	18.84658631319822	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCACGA	20	7.0277514E-4	45.0	24
CCGATTT	20	7.0277514E-4	45.0	31
CGGTCTA	20	7.0277514E-4	45.0	31
ATAACGG	50	2.1827873E-11	45.0	2
AATAGCG	25	3.8862177E-5	45.0	1
GCGTAAG	20	7.0277514E-4	45.0	1
TTGGACG	30	2.1619308E-6	44.999996	1
GCTACGA	185	0.0	43.783787	10
CGAATAT	185	0.0	41.351353	14
CTACGAA	200	0.0	40.5	11
TACGAAT	190	0.0	40.263157	12
ATAGGGA	275	0.0	40.090908	4
TTGTCGG	45	1.9228537E-8	40.000004	2
TGAACGG	45	1.9228537E-8	40.000004	2
CATACGG	35	6.2380514E-6	38.57143	2
CGTTTGG	35	6.2380514E-6	38.57143	2
AGCTACG	205	0.0	38.414635	9
ACACGAC	30	1.1385884E-4	37.499996	26
CACGACC	30	1.1385884E-4	37.499996	27
CGAGGGT	30	1.1385884E-4	37.499996	4
>>END_MODULE