FastQCFastQC Report
Sat 18 Jun 2016
SRR3550505_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550505_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477130
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30080.6304361494770817No Hit
GCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC7610.15949531574204096TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGCT6290.1318298996080733TruSeq Adapter, Index 15 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTG5570.11673967262590909TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC5190.10877538616310022TruSeq Adapter, Index 14 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCCG207.0291327E-445.0000041
CGGGCAA502.1827873E-1145.06
CGGTCTA253.8873644E-545.031
GCCGATT302.162822E-644.9999969
TACGGGT650.041.538464
CGTTTCT650.041.538461
CGTTTTT18550.041.239891
TTGCGAG501.0786607E-940.51
GCGTTAG356.240609E-638.571431
TATTGCG356.240609E-638.571431
GTTAACG356.240609E-638.571431
CACGACG301.13892136E-437.49999626
TCTAGCG301.13892136E-437.4999961
TTGCGCG301.13892136E-437.4999961
AATACGG301.13892136E-437.4999962
AGTAACG301.13892136E-437.4999961
ACGGTCT301.13892136E-437.49999630
TAAGGCG552.743036E-936.818181
TGGGCGA2450.036.7346956
CCGGATG250.002105876336.01
Produced by FastQC (version 0.11.5)