##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550505_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	477130
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.260010898497264	33.0	31.0	34.0	30.0	34.0
2	32.38590321296083	34.0	31.0	34.0	30.0	34.0
3	32.452411292519855	34.0	31.0	34.0	30.0	34.0
4	35.96394902856664	37.0	35.0	37.0	35.0	37.0
5	35.93338503133318	37.0	35.0	37.0	35.0	37.0
6	35.954217928028	37.0	35.0	37.0	35.0	37.0
7	36.112675790665016	37.0	35.0	37.0	35.0	37.0
8	36.10762894808543	37.0	35.0	37.0	35.0	37.0
9	37.69763167270975	39.0	38.0	39.0	35.0	39.0
10	37.44401525789617	39.0	37.0	39.0	35.0	39.0
11	37.37732902982415	39.0	37.0	39.0	35.0	39.0
12	37.27071028860059	39.0	37.0	39.0	34.0	39.0
13	37.21599564060109	39.0	37.0	39.0	34.0	39.0
14	38.44346194957349	40.0	38.0	41.0	34.0	41.0
15	38.506509756250914	40.0	38.0	41.0	34.0	41.0
16	38.51837863894536	40.0	38.0	41.0	34.0	41.0
17	38.54020078385346	40.0	38.0	41.0	34.0	41.0
18	38.45485507094502	40.0	38.0	41.0	34.0	41.0
19	38.37904554314338	40.0	37.0	41.0	34.0	41.0
20	38.232779326389036	40.0	37.0	41.0	34.0	41.0
21	38.18440886131662	40.0	37.0	41.0	34.0	41.0
22	38.158288097583466	40.0	37.0	41.0	34.0	41.0
23	38.12247605474399	40.0	36.0	41.0	34.0	41.0
24	38.094192358476725	40.0	36.0	41.0	34.0	41.0
25	38.07103514765368	40.0	36.0	41.0	34.0	41.0
26	37.931982897742756	40.0	36.0	41.0	34.0	41.0
27	37.86209418816675	40.0	36.0	41.0	33.0	41.0
28	37.77331964034959	40.0	36.0	41.0	33.0	41.0
29	37.732580219227465	40.0	36.0	41.0	33.0	41.0
30	37.66819944249995	40.0	36.0	41.0	33.0	41.0
31	37.52609142162513	40.0	35.0	41.0	33.0	41.0
32	37.29347976442479	40.0	35.0	41.0	32.0	41.0
33	37.12661538783979	40.0	35.0	41.0	32.0	41.0
34	36.888307169953684	40.0	35.0	41.0	31.0	41.0
35	36.83095801982688	40.0	35.0	41.0	31.0	41.0
36	36.67016536373735	39.0	35.0	41.0	31.0	41.0
37	36.622344015257895	39.0	35.0	41.0	30.0	41.0
38	36.52187454152956	39.0	35.0	41.0	30.0	41.0
39	36.4642801752143	39.0	35.0	41.0	30.0	41.0
40	36.3987487686794	39.0	35.0	41.0	30.0	41.0
41	36.276903569257854	39.0	35.0	41.0	30.0	41.0
42	36.1996248401903	39.0	35.0	41.0	30.0	41.0
43	36.13263261584893	39.0	35.0	41.0	29.0	41.0
44	35.969473728333995	39.0	35.0	41.0	29.0	41.0
45	35.89244231131977	39.0	35.0	41.0	28.0	41.0
46	35.77759730052606	38.0	35.0	41.0	28.0	41.0
47	35.67310167040429	38.0	35.0	40.0	28.0	41.0
48	35.52758367740448	38.0	35.0	40.0	27.0	41.0
49	35.431278687150254	38.0	35.0	40.0	27.0	41.0
50	35.35641649026471	38.0	35.0	40.0	27.0	41.0
51	34.12106344182927	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	6.0
12	4.0
13	6.0
14	9.0
15	19.0
16	26.0
17	51.0
18	95.0
19	198.0
20	344.0
21	627.0
22	919.0
23	1403.0
24	2347.0
25	3779.0
26	5333.0
27	5859.0
28	6196.0
29	6633.0
30	7724.0
31	9366.0
32	11615.0
33	15469.0
34	26912.0
35	36919.0
36	34102.0
37	50095.0
38	89078.0
39	161902.0
40	91.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.23075472093559	21.97619097520592	25.523861421415546	12.26919288244294
2	33.98381992329135	24.223167690147342	25.287028692389914	16.505983694171398
3	31.37362982834867	23.532161046255737	28.660532768847062	16.433676356548528
4	27.965124808752336	25.417391486596948	28.513193469285103	18.104290235365625
5	25.01393750130991	30.216502839896886	26.237293819294532	18.532265839498667
6	26.78054199065244	33.651415756712005	25.015195020225097	14.552847232410453
7	79.62525936327626	6.092469557562928	9.792509378995243	4.489761700165573
8	81.60333661685495	4.947917758262947	9.429505585479848	4.019240039402259
9	74.81503992622555	7.702093768993776	11.640643011338629	5.842223293442038
10	39.71475279273993	27.515352210089496	18.6563410391298	14.113553958040786
11	29.847840211263176	25.188313457548254	26.15995640601094	18.803889925177625
12	27.084651981640224	23.91612348835747	29.43474524762643	19.56447928237587
13	24.014000377255677	24.652400813195566	31.34764110410161	19.985957705447152
14	21.032632615848932	26.875484668748562	30.89535346760841	21.196529247794103
15	21.539831911638338	26.041120868526395	32.958313247961776	19.46073397187349
16	24.203047387504455	25.69215936956385	30.700857208727182	19.403936034204513
17	23.592312367698533	25.837402804267178	28.96610986523589	21.604174962798396
18	24.02489887452057	26.156183849265403	29.52319074466078	20.295726531553246
19	24.67210194286672	27.15486345440446	28.018359776161635	20.15467482656718
20	26.195586108607717	26.726259090813826	28.118122943432606	18.96003185714585
21	24.86093936662964	26.764194244755096	28.799069436002767	19.575796952612496
22	23.706117829522352	25.27403433026638	28.41783161821726	22.602016221994006
23	23.212122482342338	26.059774065768238	29.190157818623856	21.53794563326557
24	22.89019764005617	25.417810659568673	29.808857124892583	21.883134575482575
25	22.75124179992874	27.187978119170875	27.928237587240375	22.132542493660008
26	21.94915431852954	27.948986649340853	28.89443128707061	21.207427745059
27	23.381258776434098	26.658562655879948	28.8246389872781	21.135539580407855
28	21.058202167124264	27.919854127805838	28.986230167878773	22.03571353719112
29	23.618720264917318	26.21319137342024	28.509840085511286	21.658248276151156
30	24.091966549996858	26.315888751493304	28.302768637478255	21.289376061031582
31	24.892796512480874	27.030369081801602	26.715151007063064	21.361683398654456
32	24.38266300588938	28.116655838031562	27.18860687862847	20.31207427745059
33	23.27604636053067	28.747720746966237	26.3244817974137	21.651751095089388
34	22.578123362605577	28.052312786871504	27.85865487393373	21.510908976589192
35	23.499255967975184	28.05818120847568	26.66296397208308	21.77959885146606
36	23.45754825728837	29.35531196948421	25.735962945109296	21.451176828118122
37	23.67740448095907	28.538763020560438	27.14878544631442	20.635047052166076
38	21.54549074675665	29.677236811770374	26.835872822920376	21.941399618552595
39	23.333682644143106	27.39714543206254	26.563200804812105	22.705971118982248
40	24.009599061052544	26.716618112464108	27.605264812524887	21.66851801395846
41	22.204221071825287	27.579695261249555	26.46783895374426	23.748244713180895
42	22.33227841468782	27.065160438454928	27.23932680820741	23.363234338649843
43	22.835705153731688	25.94806446880305	27.776916144447007	23.439314233018255
44	22.665730513696477	26.554607758891706	27.074172657347056	23.70548907006476
45	22.893551023829982	26.176723324880015	26.72227694758242	24.207448703707584
46	22.762140297193636	27.6985307987341	26.563200804812105	22.97612809926016
47	22.75124179992874	26.10818854400268	28.582147423134156	22.55842223293442
48	22.09125395594492	25.847253369102763	28.45932974241821	23.60216293253411
49	22.296019952633454	26.24337182738457	28.45702429107371	23.003583928908263
50	21.1135329993922	26.1436086601136	28.5006182801333	24.24224006036091
51	21.504411795527425	25.638295642696956	27.24498564332572	25.6123069184499
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	185.0
1	396.0
2	607.0
3	2774.0
4	4941.0
5	3365.0
6	1789.0
7	1768.0
8	1747.0
9	1736.5
10	1726.0
11	1764.5
12	1803.0
13	1742.0
14	1681.0
15	1678.0
16	1675.0
17	1601.0
18	1527.0
19	1585.0
20	1643.0
21	1661.0
22	1679.0
23	1846.0
24	2013.0
25	2385.0
26	3201.0
27	3645.0
28	4581.0
29	5517.0
30	6396.0
31	7275.0
32	8372.5
33	9470.0
34	10515.5
35	11561.0
36	12530.0
37	13499.0
38	15114.0
39	16729.0
40	19243.0
41	21757.0
42	23848.0
43	25939.0
44	28954.5
45	31970.0
46	36595.5
47	41221.0
48	44255.5
49	47290.0
50	45763.0
51	44236.0
52	39194.5
53	34153.0
54	30991.5
55	27830.0
56	25852.5
57	23875.0
58	22740.5
59	21606.0
60	20362.0
61	19118.0
62	17090.0
63	15062.0
64	12921.0
65	10780.0
66	8777.5
67	6775.0
68	5754.5
69	4734.0
70	3779.5
71	2825.0
72	2409.5
73	1994.0
74	1562.5
75	935.5
76	740.0
77	554.0
78	368.0
79	236.5
80	105.0
81	85.5
82	66.0
83	54.5
84	43.0
85	36.0
86	29.0
87	18.5
88	8.0
89	5.0
90	2.0
91	2.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	477130.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.95134783535934
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.45945683249504	29.002962027144374
2	8.268053677406066	6.441036694201312
3	3.169474820893475	3.7036594861210506
4	1.987689757454647	3.0969278052558797
5	1.557636335348774	3.0336017349582267
6	1.2948922341859799	3.026267869385021
7	1.0370316088898848	2.8275645239892557
8	0.9409220452427878	2.9320145496167633
9	0.8684748869944874	3.0445440668637005
>10	6.348225457943443	38.92628740898878
>50	0.04272416752768027	1.134159302950291
>100	0.022714114381803836	1.677856398216717
>500	0.0021632489887432223	0.519471924419639
>1k	5.408122471858056E-4	0.633646207889
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3008	0.6304361494770817	No Hit
GCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC	761	0.15949531574204096	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGCT	629	0.1318298996080733	TruSeq Adapter, Index 15 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTG	557	0.11673967262590909	TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC	519	0.10877538616310022	TruSeq Adapter, Index 14 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1506926833357785	0.0
2	0.0	0.0	0.0	0.5725902793787857	0.0
3	0.0	0.0	0.0	0.8989164378680863	0.0
4	0.0	0.0	0.0	1.2332068828201959	0.0
5	0.0	0.0	0.0	2.214490809632595	0.0
6	0.0	0.0	0.0	3.0475970909395764	0.0
7	0.0	0.0	0.0	3.759352796931654	0.0
8	0.0	0.0	0.0	4.829291807264267	0.0
9	0.0	0.0	0.0	5.301490159914489	0.0
10	0.0	0.0	0.0	6.227024081487226	0.0
11	0.0	0.0	0.0	7.447236602183891	0.0
12	0.0	0.0	0.0	8.375075975101126	0.0
13	0.0	0.0	0.0	8.79068597656823	0.0
14	0.0	0.0	0.0	8.982876784104961	0.0
15	0.0	0.0	0.0	9.287615534550332	0.0
16	2.0958648586339153E-4	0.0	0.0	9.877182319284053	0.0
17	4.1917297172678307E-4	0.0	0.0	10.605076184687611	0.0
18	4.1917297172678307E-4	0.0	0.0	11.341982268983296	0.0
19	4.1917297172678307E-4	0.0	0.0	11.831366713474315	0.0
20	4.1917297172678307E-4	0.0	0.0	12.318236120134973	0.0
21	4.1917297172678307E-4	0.0	0.0	12.914928845388049	0.0
22	4.1917297172678307E-4	0.0	0.0	13.571353719112192	0.0
23	4.1917297172678307E-4	0.0	0.0	14.227569006350471	0.0
24	4.1917297172678307E-4	0.0	0.0	14.776685599312556	0.0
25	4.1917297172678307E-4	0.0	0.0	15.263974178944942	0.0
26	4.1917297172678307E-4	0.0	0.0	15.701171588455976	0.0
27	4.1917297172678307E-4	0.0	0.0	16.135644373650788	0.0
28	4.1917297172678307E-4	0.0	0.0	16.600716785781653	0.0
29	4.1917297172678307E-4	0.0	0.0	17.0796219059795	0.0
30	4.1917297172678307E-4	0.0	0.0	17.62391800976673	0.0
31	4.1917297172678307E-4	0.0	0.0	18.147465051453484	0.0
32	6.287594575901746E-4	0.0	0.0	18.646909647265943	0.0
33	6.287594575901746E-4	0.0	0.0	19.128958564751745	0.0
34	6.287594575901746E-4	0.0	0.0	19.581036614759082	0.0
35	6.287594575901746E-4	0.0	0.0	20.057845870098298	0.0
36	6.287594575901746E-4	0.0	0.0	20.551631630792446	0.0
37	6.287594575901746E-4	0.0	0.0	21.052752918491816	0.0
38	6.287594575901746E-4	0.0	0.0	21.58133003583929	0.0
39	6.287594575901746E-4	0.0	0.0	22.071133653302034	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGCCG	20	7.0291327E-4	45.000004	1
CGGGCAA	50	2.1827873E-11	45.0	6
CGGTCTA	25	3.8873644E-5	45.0	31
GCCGATT	30	2.162822E-6	44.999996	9
TACGGGT	65	0.0	41.53846	4
CGTTTCT	65	0.0	41.53846	1
CGTTTTT	1855	0.0	41.23989	1
TTGCGAG	50	1.0786607E-9	40.5	1
GCGTTAG	35	6.240609E-6	38.57143	1
TATTGCG	35	6.240609E-6	38.57143	1
GTTAACG	35	6.240609E-6	38.57143	1
CACGACG	30	1.13892136E-4	37.499996	26
TCTAGCG	30	1.13892136E-4	37.499996	1
TTGCGCG	30	1.13892136E-4	37.499996	1
AATACGG	30	1.13892136E-4	37.499996	2
AGTAACG	30	1.13892136E-4	37.499996	1
ACGGTCT	30	1.13892136E-4	37.499996	30
TAAGGCG	55	2.743036E-9	36.81818	1
TGGGCGA	245	0.0	36.734695	6
CCGGATG	25	0.0021058763	36.0	1
>>END_MODULE