FastQCFastQC Report
Sat 18 Jun 2016
SRR3550503_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550503_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340777
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52351.5361952244429642No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTT7750.22742145156509977No Hit
CGCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTCTG6830.20042432441156532No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTC5320.1561138222356556No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCT4740.13909389424755778No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG207.026293E-445.0000041
GTCGCAG207.026293E-445.0000041
ATTAGCG207.026293E-445.0000041
ATTACGG207.026293E-445.0000042
GTATACG207.026293E-445.0000041
CGCAAGT207.026293E-445.00000430
TATACGG207.026293E-445.0000042
GGTTACG207.026293E-445.0000041
GCGAGAC351.208573E-745.021
CACGACC351.208573E-745.027
CGGGCTA351.208573E-745.06
TACGAAT302.1609903E-644.99999612
GCTACGA302.1609903E-644.99999610
CGCCGGT302.1609903E-644.99999628
CGTTTTT35300.043.788951
CATAAGG556.002665E-1140.9090922
GGACCGA1100.040.9090928
GACACGA403.4486766E-739.37500425
GTCAAGC403.4486766E-739.37500416
CGAGACA403.4486766E-739.37500422