##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550503_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	340777
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.314551744982786	33.0	31.0	34.0	30.0	34.0
2	32.466862493654205	34.0	31.0	34.0	31.0	34.0
3	32.45075225147237	34.0	31.0	34.0	30.0	34.0
4	36.00685198825038	37.0	35.0	37.0	35.0	37.0
5	35.98244599840952	37.0	35.0	37.0	35.0	37.0
6	36.00821064801909	37.0	35.0	37.0	35.0	37.0
7	36.15200556375577	37.0	35.0	37.0	35.0	37.0
8	36.16766096303448	37.0	37.0	37.0	35.0	37.0
9	37.77357039941076	39.0	38.0	39.0	35.0	39.0
10	37.538399011670386	39.0	37.0	39.0	35.0	39.0
11	37.477030433391924	39.0	37.0	39.0	35.0	39.0
12	37.405006793298845	39.0	37.0	39.0	35.0	39.0
13	37.382170745091365	39.0	37.0	39.0	35.0	39.0
14	38.65621506146248	40.0	38.0	41.0	35.0	41.0
15	38.697940295266406	40.0	38.0	41.0	35.0	41.0
16	38.707119318498606	40.0	38.0	41.0	35.0	41.0
17	38.714634497046454	40.0	38.0	41.0	35.0	41.0
18	38.513083336023264	40.0	38.0	41.0	35.0	41.0
19	38.32697922688445	40.0	37.0	41.0	34.0	41.0
20	38.06593167966148	40.0	35.0	41.0	34.0	41.0
21	38.00707794246677	40.0	35.0	41.0	34.0	41.0
22	37.98964131969	40.0	35.0	41.0	34.0	41.0
23	37.96622131188431	40.0	35.0	41.0	34.0	41.0
24	37.9281671004792	40.0	35.0	41.0	34.0	41.0
25	37.892715764268125	40.0	35.0	41.0	34.0	41.0
26	37.773543989177675	40.0	35.0	41.0	34.0	41.0
27	37.71356928431203	40.0	35.0	41.0	34.0	41.0
28	37.61233592642696	40.0	35.0	41.0	33.0	41.0
29	37.52695457733356	40.0	35.0	41.0	33.0	41.0
30	37.43151973284582	40.0	35.0	41.0	33.0	41.0
31	37.20249899494391	40.0	35.0	41.0	33.0	41.0
32	36.812959794821836	39.0	35.0	41.0	31.0	41.0
33	36.43209782350335	39.0	35.0	41.0	30.0	41.0
34	35.98995237354634	39.0	35.0	41.0	27.0	41.0
35	35.80264806603732	39.0	35.0	41.0	25.0	41.0
36	35.60889379271488	39.0	35.0	41.0	24.0	41.0
37	35.52928748125607	39.0	35.0	41.0	24.0	41.0
38	35.4214544995701	39.0	35.0	41.0	23.0	41.0
39	35.330717742101136	39.0	35.0	41.0	23.0	41.0
40	35.239250301516826	39.0	35.0	41.0	23.0	41.0
41	35.11496667908926	39.0	35.0	41.0	22.0	41.0
42	35.02858761007932	39.0	35.0	41.0	22.0	41.0
43	34.94634907872304	39.0	35.0	41.0	21.0	41.0
44	34.76091989776305	38.0	34.0	41.0	20.0	41.0
45	34.73993256587152	38.0	34.0	41.0	20.0	41.0
46	34.66447559547739	38.0	34.0	40.0	20.0	41.0
47	34.60196257376525	38.0	34.0	40.0	20.0	41.0
48	34.461474806104874	38.0	34.0	40.0	20.0	41.0
49	34.3763692972237	38.0	34.0	40.0	20.0	41.0
50	34.3088794138102	38.0	34.0	40.0	20.0	41.0
51	33.21006699395792	37.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	4.0
11	4.0
12	1.0
13	4.0
14	3.0
15	8.0
16	16.0
17	47.0
18	91.0
19	167.0
20	344.0
21	529.0
22	875.0
23	1447.0
24	2498.0
25	4489.0
26	6689.0
27	7567.0
28	7132.0
29	6379.0
30	6458.0
31	6869.0
32	7945.0
33	10166.0
34	17582.0
35	23747.0
36	22569.0
37	32045.0
38	59105.0
39	115920.0
40	76.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.03852372665995	20.780158285330288	23.970220994961515	19.21109699304824
2	38.549549998972935	22.890629355854415	23.113062207836794	15.446758437335854
3	28.51835657922923	22.05019704968352	34.28018909726889	15.151257273818361
4	25.514632736364252	23.65975403269587	34.01021782573355	16.81539540520634
5	23.78887072777799	28.0652743583047	31.28409487729512	16.86176003662219
6	24.538334453322847	31.54144792635653	29.994982055713855	13.925235564606767
7	74.9290591794634	5.231573727100127	15.781581503446535	4.057785589989934
8	77.33356417833363	4.278457759766651	15.063223163535097	3.3247548983646196
9	70.79732493683552	7.032458176461439	17.020221435132065	5.149995451570969
10	35.83721906114556	28.036809995979777	23.450819744290254	12.67515119858441
11	26.606842597945285	22.356849200503557	33.571221062454335	17.46508713909683
12	24.435627991325706	20.976180904227693	35.70868926013199	18.87950184431461
13	21.96715153898297	21.557792926165792	37.545667694709444	18.929387840141793
14	19.545626612124643	24.10608697183202	36.81762560266685	19.530660813376493
15	19.88807930112654	24.538334453322847	37.73141966740713	17.842166578143477
16	23.035005296718968	23.943810761876534	36.041458197002726	16.97972574440176
17	22.289943276688273	24.79304647907576	34.285177696851605	18.63183254738436
18	22.206017424884894	24.689459675975783	34.413120603796614	18.6914022953427
19	22.49887756509389	25.93191441910692	33.408651405464575	18.16055661033462
20	23.241298561816084	25.44537923627475	33.78220948009989	17.53111272180928
21	23.38919586709197	25.351476185305934	34.097665041948254	17.161662905653845
22	22.440481605272655	23.224572080862266	34.131998344958724	20.20294796890635
23	21.30043987710438	24.891351235558737	34.476505163200564	19.33170372413631
24	21.321861510606645	23.69790214715195	35.45544446955047	19.52479187269094
25	21.476801544705186	25.443031660000525	33.10405338388448	19.976113411409806
26	20.243150212602377	26.56546656611215	33.468808047491464	19.722575173794006
27	21.24468494059165	25.410165592161444	33.532486053929695	19.812663413317214
28	20.266919422378855	26.081278959554194	33.97735175789446	19.674449860172487
29	21.48501806166496	24.899567752518507	33.10581406609014	20.50960011972639
30	22.08423690565971	24.94270446655731	33.02834404904087	19.944714578742108
31	23.147688957881545	25.06360464467966	32.02915689732582	19.75954950011298
32	22.727766251830374	25.744988658272128	31.686997655358194	19.8402474345393
33	22.652350364021046	26.00028757809359	31.141773065670513	20.20558899221485
34	21.548696068103187	26.887671409748897	31.761826649098975	19.801805873048945
35	21.69894094965329	27.639776158602253	30.391722446057102	20.269560445687357
36	22.000311053856333	29.13987739782908	29.059766357471307	19.800045190843278
37	23.039113555198853	28.597000384415615	29.319760429841217	19.044125630544315
38	21.581562135942274	28.081707392224242	29.72677146638418	20.609959005449312
39	22.564903147806337	26.730677246410412	29.715913926115906	20.988505679667348
40	22.374456022560206	26.674922309897674	30.54255422167576	20.408067445866358
41	20.68273386995014	27.92882148736564	30.021392288798832	21.367052353885384
42	21.78286680145667	27.946428309422288	29.185948582210653	21.084756306910386
43	22.479803507865846	26.095070970165242	30.075093096071626	21.35003242589729
44	22.17315135704581	26.40729861463655	29.706523621019027	21.713026407298614
45	21.524046517223873	25.6067751051274	30.227979000930226	22.6411993767185
46	21.55573879692585	27.02500462179079	29.17919930042227	22.24005728086109
47	21.638490860592118	26.124122226558715	30.665801976072327	21.571584936776837
48	21.497342837104615	25.489396291416384	31.091006728740496	21.922254142738506
49	21.414884220472626	25.339444856900556	30.893810321705985	22.351860600920837
50	19.880743125269603	25.23585805380058	31.810538856789044	23.072859964140772
51	20.326782617371478	24.55212646393389	31.446664534284885	23.674426384409745
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	114.0
1	260.0
2	406.0
3	4917.5
4	9429.0
5	6373.5
6	3318.0
7	3286.0
8	3254.0
9	3259.5
10	3265.0
11	3180.5
12	3096.0
13	3002.0
14	2908.0
15	2694.5
16	2481.0
17	2388.0
18	2295.0
19	2177.5
20	2060.0
21	1992.5
22	1925.0
23	1954.5
24	1984.0
25	2249.0
26	2969.5
27	3425.0
28	4057.5
29	4690.0
30	5511.5
31	6333.0
32	7116.0
33	7899.0
34	8556.0
35	9213.0
36	10038.5
37	10864.0
38	11716.5
39	12569.0
40	14340.5
41	16112.0
42	16869.5
43	17627.0
44	18895.0
45	20163.0
46	23232.0
47	26301.0
48	27337.5
49	28374.0
50	28218.0
51	28062.0
52	25257.5
53	22453.0
54	20351.5
55	18250.0
56	16974.0
57	15698.0
58	14874.0
59	14050.0
60	13080.5
61	12111.0
62	10902.5
63	9694.0
64	8290.5
65	6887.0
66	5500.0
67	4113.0
68	3319.0
69	2525.0
70	2242.5
71	1960.0
72	1474.0
73	988.0
74	809.5
75	497.0
76	363.0
77	277.0
78	191.0
79	145.0
80	99.0
81	67.5
82	36.0
83	30.0
84	24.0
85	15.0
86	6.0
87	8.0
88	10.0
89	7.5
90	5.0
91	3.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	340777.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.862048798281506
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.16190326681297	34.0595448673479
2	9.483913193783641	9.078390321590769
3	5.31817631855563	7.636164434297241
4	3.6882166483178387	7.061024208016906
5	2.7631342871241293	6.612463404326993
6	2.0232643834078563	5.810254719029507
7	1.5371141197406444	5.149860170528798
8	1.1294211649232977	4.324512872749666
9	0.8170742751805096	3.5196163947459036
>10	2.0422158830002703	12.707593026265416
>50	0.01962287401556042	0.6503897354339834
>100	0.013490725885697788	1.2696687705268106
>500	0.0018396444389587891	0.584059374329254
>1k	0.0	0.0
>5k	6.132148129862631E-4	1.5364577008108768
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5235	1.5361952244429642	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTT	775	0.22742145156509977	No Hit
CGCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTCTG	683	0.20042432441156532	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTC	532	0.1561138222356556	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCT	474	0.13909389424755778	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08950134545465216	0.0
2	0.0	0.0	0.0	0.3180965851568621	0.0
3	0.0	0.0	0.0	0.6696461322213647	0.0
4	0.0	0.0	0.0	0.9501814969907006	0.0
5	0.0	0.0	0.0	1.7251751145177052	0.0
6	0.0	0.0	0.0	2.7669120862030008	0.0
7	2.934470342775481E-4	0.0	0.0	3.524005434639075	0.0
8	2.934470342775481E-4	0.0	0.0	4.58129509914108	0.0
9	5.868940685550962E-4	0.0	0.0	5.194012506712601	0.0
10	5.868940685550962E-4	0.0	0.0	5.99600325139314	0.0
11	5.868940685550962E-4	0.0	0.0	6.8200025236444946	0.0
12	5.868940685550962E-4	0.0	0.0	7.515471994882284	0.0
13	5.868940685550962E-4	0.0	0.0	7.901648291991537	0.0
14	5.868940685550962E-4	0.0	0.0	8.120559779562587	0.0
15	5.868940685550962E-4	0.0	0.0	8.341818843407859	0.0
16	5.868940685550962E-4	0.0	0.0	8.687792896821088	0.0
17	5.868940685550962E-4	0.0	0.0	9.110356626180758	0.0
18	5.868940685550962E-4	0.0	0.0	9.558156800488296	0.0
19	5.868940685550962E-4	0.0	0.0	9.880361644125044	0.0
20	5.868940685550962E-4	0.0	0.0	10.188774477150746	0.0
21	5.868940685550962E-4	0.0	0.0	10.550594670414963	0.0
22	5.868940685550962E-4	0.0	0.0	10.970223929431857	0.0
23	5.868940685550962E-4	0.0	0.0	11.390733529551584	0.0
24	5.868940685550962E-4	0.0	0.0	11.737587924067645	0.0
25	5.868940685550962E-4	0.0	0.0	12.038664581236409	0.0
26	5.868940685550962E-4	0.0	0.0	12.32829680406835	0.0
27	8.803411028326442E-4	0.0	0.0	12.620570050208787	0.0
28	0.0011737881371101924	0.0	0.0	12.918712237034777	0.0
29	0.0011737881371101924	0.0	0.0	13.2555894323854	0.0
30	0.0011737881371101924	0.0	0.0	13.639124706186157	0.0
31	0.0011737881371101924	0.0	0.0	13.999771111313263	0.0
32	0.0011737881371101924	0.0	0.0	14.37010126857153	0.0
33	0.0011737881371101924	0.0	0.0	14.710499828333486	0.0
34	0.0011737881371101924	0.0	0.0	15.054419752506771	0.0
35	0.0011737881371101924	0.0	0.0	15.388069030480343	0.0
36	0.0011737881371101924	0.0	0.0	15.736977554236349	0.0
37	0.0011737881371101924	0.0	0.0	16.11728491066005	0.0
38	0.0011737881371101924	0.0	0.0	16.5204811357574	0.0
39	0.0011737881371101924	0.0	0.0	16.928079066368916	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	20	7.026293E-4	45.000004	1
GTCGCAG	20	7.026293E-4	45.000004	1
ATTAGCG	20	7.026293E-4	45.000004	1
ATTACGG	20	7.026293E-4	45.000004	2
GTATACG	20	7.026293E-4	45.000004	1
CGCAAGT	20	7.026293E-4	45.000004	30
TATACGG	20	7.026293E-4	45.000004	2
GGTTACG	20	7.026293E-4	45.000004	1
GCGAGAC	35	1.208573E-7	45.0	21
CACGACC	35	1.208573E-7	45.0	27
CGGGCTA	35	1.208573E-7	45.0	6
TACGAAT	30	2.1609903E-6	44.999996	12
GCTACGA	30	2.1609903E-6	44.999996	10
CGCCGGT	30	2.1609903E-6	44.999996	28
CGTTTTT	3530	0.0	43.78895	1
CATAAGG	55	6.002665E-11	40.909092	2
GGACCGA	110	0.0	40.909092	8
GACACGA	40	3.4486766E-7	39.375004	25
GTCAAGC	40	3.4486766E-7	39.375004	16
CGAGACA	40	3.4486766E-7	39.375004	22
>>END_MODULE