SRR3550499_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550499_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	568265
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1454	0.2558665411383773	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1124	0.19779504280573323	No Hit
GCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	999	0.17579826313427716	TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	747	0.13145275531662165	TruSeq Adapter, Index 16 (95% over 21bp)
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	687	0.12089430107432271	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	632	0.11121571801888204	No Hit
CTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGCT	595	0.10470467123613104	TruSeq Adapter, Index 13 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTA	20	7.030271E-4	45.000004	41
AATTGCG	25	3.8883067E-5	45.000004	1
GATATCG	20	7.030271E-4	45.000004	9
CGATGCA	40	6.8048394E-9	45.000004	10
GTGTACG	35	1.2104829E-7	45.0	1
TCGATAG	30	2.163557E-6	44.999996	1
CTACGAA	155	0.0	43.548386	11
GCTACGA	160	0.0	42.187504	10
CAATCAC	135	0.0	41.666664	42
TTGTGCG	55	6.002665E-11	40.909092	1
CTAAGCG	50	1.0786607E-9	40.500004	1
AATACGG	45	1.9254003E-8	40.000004	2
CGAATAT	170	0.0	39.705883	14
ACGTTAG	40	3.4540972E-7	39.375004	1
GTTAACG	40	3.4540972E-7	39.375004	1
TAATGCG	40	3.4540972E-7	39.375004	1
GCGTATG	40	3.4540972E-7	39.375004	1
CGTAAGG	110	0.0	38.86364	2
TACGAAT	175	0.0	38.571426	12
ACTCAAT	35	6.2427152E-6	38.571426	30