##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550499_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	568265
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33321953665983	33.0	31.0	34.0	30.0	34.0
2	32.43420059303318	34.0	31.0	34.0	31.0	34.0
3	32.538556835279316	34.0	31.0	34.0	31.0	34.0
4	36.02674984382286	37.0	35.0	37.0	35.0	37.0
5	35.98116899685886	37.0	35.0	37.0	35.0	37.0
6	35.99433714904138	37.0	35.0	37.0	35.0	37.0
7	36.11884244146657	37.0	35.0	37.0	35.0	37.0
8	36.084010100921226	37.0	35.0	37.0	35.0	37.0
9	37.62209180575964	39.0	38.0	39.0	35.0	39.0
10	37.46667487879775	39.0	37.0	39.0	35.0	39.0
11	37.40038714332222	39.0	37.0	39.0	35.0	39.0
12	37.270796195436986	39.0	37.0	39.0	34.0	39.0
13	37.191398379277274	39.0	37.0	39.0	34.0	39.0
14	38.39271290683044	40.0	38.0	41.0	34.0	41.0
15	38.4659076311228	40.0	38.0	41.0	34.0	41.0
16	38.50011526312548	40.0	38.0	41.0	34.0	41.0
17	38.52003026756883	40.0	38.0	41.0	34.0	41.0
18	38.47529585668658	40.0	38.0	41.0	34.0	41.0
19	38.441251880724664	40.0	37.0	41.0	34.0	41.0
20	38.33833862722497	40.0	37.0	41.0	34.0	41.0
21	38.270704688833554	40.0	37.0	41.0	34.0	41.0
22	38.25337650567957	40.0	37.0	41.0	34.0	41.0
23	38.21731234547262	40.0	37.0	41.0	34.0	41.0
24	38.19247006238287	40.0	37.0	41.0	34.0	41.0
25	38.132413574652674	40.0	37.0	41.0	34.0	41.0
26	38.00437120005631	40.0	37.0	41.0	34.0	41.0
27	37.95020984927807	40.0	36.0	41.0	34.0	41.0
28	37.86167545071402	40.0	36.0	41.0	33.0	41.0
29	37.80736628157638	40.0	36.0	41.0	33.0	41.0
30	37.78936411709326	40.0	36.0	41.0	33.0	41.0
31	37.7029326106658	40.0	36.0	41.0	33.0	41.0
32	37.56470132772562	40.0	36.0	41.0	33.0	41.0
33	37.459620071621515	40.0	36.0	41.0	33.0	41.0
34	37.311296666168076	40.0	35.0	41.0	32.0	41.0
35	37.28800823559431	40.0	35.0	41.0	32.0	41.0
36	37.146644611228915	40.0	35.0	41.0	32.0	41.0
37	37.13434929126376	40.0	35.0	41.0	32.0	41.0
38	37.046809147140856	39.0	35.0	41.0	32.0	41.0
39	36.94879677615197	39.0	35.0	41.0	31.0	41.0
40	36.85318469376083	39.0	35.0	41.0	31.0	41.0
41	36.730887878014656	39.0	35.0	41.0	31.0	41.0
42	36.649723280511736	39.0	35.0	41.0	31.0	41.0
43	36.581156678662246	39.0	35.0	41.0	31.0	41.0
44	36.37338565633991	39.0	35.0	41.0	30.0	41.0
45	36.32919852533589	39.0	35.0	41.0	30.0	41.0
46	36.22925395722066	38.0	35.0	41.0	30.0	41.0
47	36.14166278056892	38.0	35.0	40.0	30.0	41.0
48	36.032743526347744	38.0	35.0	40.0	30.0	41.0
49	35.93323185485645	38.0	35.0	40.0	29.0	41.0
50	35.83082540716039	38.0	35.0	40.0	29.0	41.0
51	34.57544631465954	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	11.0
11	5.0
12	4.0
13	5.0
14	9.0
15	18.0
16	28.0
17	83.0
18	133.0
19	242.0
20	385.0
21	599.0
22	976.0
23	1454.0
24	2102.0
25	3023.0
26	4102.0
27	5057.0
28	5955.0
29	6925.0
30	8432.0
31	10524.0
32	13502.0
33	18367.0
34	33283.0
35	44971.0
36	41732.0
37	61498.0
38	105624.0
39	199037.0
40	175.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.14846946407046	21.559835640062293	24.55966846453679	11.732026431330453
2	30.971289803172812	25.224675107564252	25.347505125249665	18.456529964013267
3	32.40055255910534	24.817294748048884	26.07058326660977	16.711569426236
4	28.97433415747934	26.109649547306272	25.63645482301391	19.27956147220047
5	25.748022489507537	31.16186990224631	23.62894072307814	19.46116688516801
6	27.60895004971272	34.451884244146655	22.4653990655768	15.473766640563822
7	80.56470132772561	6.917371296842142	7.615989019207587	4.901938356224648
8	81.48962191935101	6.157162591396619	7.614581225308614	4.738634263943759
9	74.46367451805057	9.018679665297	10.037042576966732	6.48060323968571
10	42.03883751418792	27.166022894248282	16.260547455852464	14.534592135711332
11	31.718828363527578	25.060491144096503	23.323273472763585	19.897407019612327
12	29.71096231511707	22.989098395994827	26.949574582281155	20.350364706606953
13	25.271308280467743	24.797057710751147	27.979551793617414	21.952082215163703
14	20.808073698010613	26.999551265694706	28.738000756689217	23.454374279605467
15	21.107229901542414	25.860118078713278	31.631897090265987	21.400754929478325
16	23.485873668094992	25.367918136784773	29.583732941497367	21.56247525362287
17	23.275056531723756	25.817180364794595	27.234124924111107	23.673638179370542
18	24.35782601427151	26.086948870685333	27.317184764150525	22.23804035089263
19	25.54987549822706	26.867218639191222	25.496907252778193	22.085998609803525
20	27.198578128162037	25.999665648948994	26.43379409254485	20.367962130344118
21	25.8456881912488	26.791549717121416	26.35214204640441	21.010620045225377
22	24.718045278171275	25.079496361732645	26.000017597423735	24.202440762672346
23	24.556676902501472	26.034156599473835	26.63229303230007	22.776873465724616
24	24.44950859194214	24.372079927498614	27.640273463964878	23.53813801659437
25	23.325209189374675	26.7109535164052	25.9232928299297	24.040544464290427
26	22.896008024425225	28.10185388859071	26.161913895805654	22.84022419117841
27	23.607119917644056	27.100384503708657	26.309732255197837	22.982763323449447
28	21.397939341680377	27.867280230174302	26.948518736856926	23.786261691288395
29	22.719857812816205	26.616103402461878	26.48359480172103	24.180443983000888
30	22.763499423684372	26.24039840567341	26.570350100745248	24.425752069896966
31	23.825855894697014	26.947990814144806	24.47291316551257	24.753240125645608
32	23.704785619385323	27.08014746641092	25.102724961065697	24.11234195313806
33	23.10999269706915	27.155992362718095	25.080376232919498	24.65363870729325
34	21.736513774383432	26.576333224815883	26.666783982824917	25.020369017975767
35	22.160435712211733	26.29266275417279	26.32996929249558	25.2169322411199
36	22.010329687733716	28.945650356787766	24.82943697042753	24.21458298505099
37	22.74079874706343	27.840004223381698	25.697693857619246	23.72150317193563
38	21.67228317774278	27.126956613551776	25.931387644848797	25.269372563856653
39	23.09327514451884	26.088708613059048	26.62349432043149	24.19452192199062
40	23.217336981865856	25.024944348147432	28.38482046228432	23.37289820770239
41	20.51014931414041	26.65868916790582	27.226382057666758	25.604779460287013
42	21.50510765223971	26.731190553702938	26.908220636498815	24.855481157558536
43	22.89460023052625	25.61217037825662	26.93848820532674	24.554741185890386
44	22.371076874345597	25.74819846374491	26.340351772500505	25.540372889408992
45	21.998715388067186	24.987461835587272	26.31342771418264	26.7003950621629
46	21.369959437938284	26.690716479107458	26.275417278910368	25.66390680404389
47	21.088224683906276	25.84674403667303	28.38024513211266	24.684786147308035
48	21.377526330145265	24.79969732431172	28.03656744652583	25.786208899017183
49	21.771708621857762	25.46294422496547	27.85742567288149	24.907921480295283
50	20.03959420340862	25.812956983097674	27.912153660704075	26.235295152789632
51	20.266249021143302	25.08213597529322	27.066949398608042	27.584665604955433
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	145.0
1	524.5
2	904.0
3	1862.0
4	2820.0
5	1957.0
6	1094.0
7	1069.0
8	1044.0
9	1010.0
10	976.0
11	1007.5
12	1039.0
13	1038.5
14	1038.0
15	1105.0
16	1172.0
17	1261.5
18	1351.0
19	1239.5
20	1128.0
21	1261.0
22	1394.0
23	1555.5
24	1717.0
25	2338.0
26	3533.5
27	4108.0
28	5351.0
29	6594.0
30	7982.0
31	9370.0
32	10445.5
33	11521.0
34	13115.0
35	14709.0
36	15404.5
37	16100.0
38	17688.0
39	19276.0
40	21683.5
41	24091.0
42	27067.0
43	30043.0
44	32736.0
45	35429.0
46	38669.5
47	41910.0
48	45142.0
49	48374.0
50	47969.0
51	47564.0
52	42801.5
53	38039.0
54	36540.0
55	35041.0
56	33254.5
57	31468.0
58	30695.0
59	29922.0
60	28797.5
61	27673.0
62	26051.5
63	24430.0
64	21385.5
65	18341.0
66	15188.5
67	12036.0
68	10436.0
69	8836.0
70	7181.0
71	5526.0
72	4704.5
73	3883.0
74	3060.5
75	1900.0
76	1562.0
77	1162.5
78	763.0
79	561.0
80	359.0
81	265.0
82	171.0
83	113.0
84	55.0
85	40.5
86	26.0
87	20.5
88	15.0
89	10.0
90	5.0
91	4.5
92	4.0
93	2.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	568265.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.604790172843728
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.51859449261825	22.603301723958964
2	8.380192466984992	5.297084490542127
3	3.0762283122486958	2.9167065099714367
4	1.9023447576437444	2.404928276069592
5	1.4395844519901018	2.2748882270617696
6	1.240173839342377	2.35172603821595
7	1.0688774625816244	2.36471535477617
8	1.0358541704866817	2.6190362966317338
9	0.9697771712211675	2.758464360977305
>10	9.261572739371863	49.82903694971148
>50	0.0687770506167237	1.4804026822064094
>100	0.03354978078864469	1.9022388052130141
>500	0.003354978078864469	0.7418802029249567
>1k	0.0011183260262881563	0.4555900817390515
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1454	0.2558665411383773	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1124	0.19779504280573323	No Hit
GCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	999	0.17579826313427716	TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	747	0.13145275531662165	TruSeq Adapter, Index 16 (95% over 21bp)
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	687	0.12089430107432271	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	632	0.11121571801888204	No Hit
CTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGCT	595	0.10470467123613104	TruSeq Adapter, Index 13 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7597423737164878E-4	0.0	0.0	0.12001442988746447	0.0
2	1.7597423737164878E-4	0.0	0.0	0.5729721168820885	0.0
3	1.7597423737164878E-4	0.0	0.0	0.8228555339498298	0.0
4	1.7597423737164878E-4	0.0	0.0	1.134329934097648	0.0
5	1.7597423737164878E-4	0.0	0.0	2.0534433758897697	0.0
6	1.7597423737164878E-4	0.0	0.0	2.6931097287357133	0.0
7	1.7597423737164878E-4	0.0	0.0	3.3121870958091737	0.0
8	1.7597423737164878E-4	0.0	0.0	4.257344724732299	0.0
9	1.7597423737164878E-4	0.0	0.0	4.637977000167176	0.0
10	1.7597423737164878E-4	0.0	0.0	5.380412307638162	0.0
11	1.7597423737164878E-4	0.0	0.0	6.341935540636851	0.0
12	1.7597423737164878E-4	0.0	0.0	7.150889109834321	0.0
13	1.7597423737164878E-4	0.0	0.0	7.477321320158729	0.0
14	1.7597423737164878E-4	0.0	0.0	7.624259808364055	0.0
15	1.7597423737164878E-4	0.0	0.0	7.896140005103253	0.0
16	1.7597423737164878E-4	0.0	0.0	8.429517918576721	0.0
17	1.7597423737164878E-4	0.0	0.0	9.055458280907676	0.0
18	1.7597423737164878E-4	0.0	0.0	9.681046694763886	0.0
19	1.7597423737164878E-4	0.0	0.0	10.078220548511698	0.0
20	1.7597423737164878E-4	0.0	0.0	10.484721036840206	0.0
21	1.7597423737164878E-4	0.0	0.0	10.998213861490678	0.0
22	1.7597423737164878E-4	0.0	0.0	11.512234608853264	0.0
23	1.7597423737164878E-4	0.0	0.0	12.038749527069237	0.0
24	1.7597423737164878E-4	0.0	0.0	12.470766279816635	0.0
25	1.7597423737164878E-4	0.0	0.0	12.855621936948431	0.0
26	1.7597423737164878E-4	0.0	0.0	13.201587287621093	0.0
27	1.7597423737164878E-4	0.0	0.0	13.56321434541983	0.0
28	1.7597423737164878E-4	0.0	0.0	13.925369325930683	0.0
29	1.7597423737164878E-4	0.0	0.0	14.288404177628395	0.0
30	1.7597423737164878E-4	0.0	0.0	14.735906663264497	0.0
31	3.5194847474329756E-4	0.0	0.0	15.131672723113336	0.0
32	3.5194847474329756E-4	0.0	0.0	15.544156335512481	0.0
33	3.5194847474329756E-4	0.0	0.0	15.929891863831136	0.0
34	3.5194847474329756E-4	0.0	0.0	16.288175411119813	0.0
35	5.279227121149463E-4	0.0	0.0	16.697843435721012	0.0
36	5.279227121149463E-4	0.0	0.0	17.097832877266768	0.0
37	5.279227121149463E-4	0.0	0.0	17.50362946864579	0.0
38	5.279227121149463E-4	0.0	0.0	17.915761132570193	0.0
39	7.038969494865951E-4	0.0	0.0	18.349185679216564	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTA	20	7.030271E-4	45.000004	41
AATTGCG	25	3.8883067E-5	45.000004	1
GATATCG	20	7.030271E-4	45.000004	9
CGATGCA	40	6.8048394E-9	45.000004	10
GTGTACG	35	1.2104829E-7	45.0	1
TCGATAG	30	2.163557E-6	44.999996	1
CTACGAA	155	0.0	43.548386	11
GCTACGA	160	0.0	42.187504	10
CAATCAC	135	0.0	41.666664	42
TTGTGCG	55	6.002665E-11	40.909092	1
CTAAGCG	50	1.0786607E-9	40.500004	1
AATACGG	45	1.9254003E-8	40.000004	2
CGAATAT	170	0.0	39.705883	14
ACGTTAG	40	3.4540972E-7	39.375004	1
GTTAACG	40	3.4540972E-7	39.375004	1
TAATGCG	40	3.4540972E-7	39.375004	1
GCGTATG	40	3.4540972E-7	39.375004	1
CGTAAGG	110	0.0	38.86364	2
TACGAAT	175	0.0	38.571426	12
ACTCAAT	35	6.2427152E-6	38.571426	30
>>END_MODULE