FastQCFastQC Report
Sat 18 Jun 2016
SRR3550497_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550497_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446177
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9970.22345392075342296No Hit
GCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTCTGC6480.1452338421747423No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT5940.1331310219935138No Hit
CCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTCTGC5690.1275278645022043No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTAG351.2097007E-745.0000041
TAGCGAG207.028642E-445.01
GCGTTAG207.028642E-445.01
GTATGCG253.8869563E-545.01
TTAAGCG207.028642E-445.01
AGCGCAT207.028642E-445.027
GTATACG406.7993824E-945.01
CGGTCTA253.8869563E-545.031
CACAACG406.7993824E-945.012
GTAACGG551.8189894E-1245.02
TTGACGG302.1625056E-644.9999962
AGTAACG302.1625056E-644.9999961
AACACGT800.042.187541
TCGTCCC750.042.038
TATACGG650.041.538462
CTCGTCC800.039.37537
CCTCGTC800.039.37536
ACCGGGT356.2396975E-638.571434
AGACACG1050.038.5714324
TAACGGG1000.038.253