##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550497_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	446177
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.397207834558934	33.0	31.0	34.0	31.0	34.0
2	32.51349352387057	34.0	31.0	34.0	31.0	34.0
3	32.60608906330896	34.0	31.0	34.0	31.0	34.0
4	36.07399081530424	37.0	35.0	37.0	35.0	37.0
5	36.040672199597914	37.0	35.0	37.0	35.0	37.0
6	36.04917331014373	37.0	35.0	37.0	35.0	37.0
7	36.19343668544098	37.0	36.0	37.0	35.0	37.0
8	36.194537145572276	37.0	37.0	37.0	35.0	37.0
9	37.77618299464114	39.0	38.0	39.0	35.0	39.0
10	37.529339253256	39.0	37.0	39.0	35.0	39.0
11	37.46925995737118	39.0	37.0	39.0	35.0	39.0
12	37.370832651615835	39.0	37.0	39.0	35.0	39.0
13	37.31243654424141	39.0	37.0	39.0	34.0	39.0
14	38.549654061056486	40.0	38.0	41.0	34.0	41.0
15	38.62148654009508	40.0	38.0	41.0	35.0	41.0
16	38.65398261228167	40.0	38.0	41.0	35.0	41.0
17	38.65889097824406	40.0	38.0	41.0	35.0	41.0
18	38.61944474950524	40.0	38.0	41.0	35.0	41.0
19	38.58028316116698	40.0	38.0	41.0	35.0	41.0
20	38.487004036514655	40.0	38.0	41.0	34.0	41.0
21	38.43889084376828	40.0	38.0	41.0	34.0	41.0
22	38.40831105144371	40.0	37.0	41.0	34.0	41.0
23	38.38292874800808	40.0	37.0	41.0	34.0	41.0
24	38.36437557292285	40.0	37.0	41.0	34.0	41.0
25	38.331429006873954	40.0	37.0	41.0	34.0	41.0
26	38.201641949271256	40.0	37.0	41.0	34.0	41.0
27	38.14271466256665	40.0	37.0	41.0	34.0	41.0
28	38.071487324537124	40.0	37.0	41.0	34.0	41.0
29	38.01539523552312	40.0	37.0	41.0	34.0	41.0
30	38.01124441645356	40.0	37.0	41.0	34.0	41.0
31	37.92427892966244	40.0	37.0	41.0	34.0	41.0
32	37.754998576797995	40.0	36.0	41.0	33.0	41.0
33	37.67826221432301	40.0	36.0	41.0	33.0	41.0
34	37.534904309276364	40.0	36.0	41.0	33.0	41.0
35	37.49970527391596	40.0	36.0	41.0	33.0	41.0
36	37.376592697516905	40.0	36.0	41.0	33.0	41.0
37	37.345524309859094	40.0	35.0	41.0	32.0	41.0
38	37.23637704319138	40.0	35.0	41.0	32.0	41.0
39	37.16718925448869	40.0	35.0	41.0	32.0	41.0
40	37.097326845624046	40.0	35.0	41.0	32.0	41.0
41	36.95655311681239	39.0	35.0	41.0	31.0	41.0
42	36.9045132312961	39.0	35.0	41.0	31.0	41.0
43	36.8468096741876	39.0	35.0	41.0	31.0	41.0
44	36.685232542242204	39.0	35.0	41.0	31.0	41.0
45	36.62528099834819	39.0	35.0	41.0	31.0	41.0
46	36.50235444677785	39.0	35.0	41.0	31.0	41.0
47	36.417822971600955	39.0	35.0	41.0	31.0	41.0
48	36.290631296548234	39.0	35.0	41.0	30.0	41.0
49	36.21601516886796	39.0	35.0	41.0	30.0	41.0
50	36.12756372471015	38.0	35.0	40.0	30.0	41.0
51	34.89098048532309	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	2.0
11	2.0
12	2.0
13	4.0
14	9.0
15	7.0
16	17.0
17	40.0
18	85.0
19	141.0
20	252.0
21	449.0
22	672.0
23	973.0
24	1491.0
25	2098.0
26	2883.0
27	3602.0
28	4370.0
29	4877.0
30	6094.0
31	7769.0
32	9789.0
33	13401.0
34	24637.0
35	33581.0
36	30516.0
37	45551.0
38	82952.0
39	169754.0
40	154.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.444935530069905	22.506090632193054	26.022184021139594	11.026789816597448
2	31.37454418313808	25.27987771669091	25.614722408371566	17.730855691799444
3	31.012356082899835	24.66689228714165	27.525399112908104	16.795352517050407
4	29.144935754196204	26.168090242213292	26.17145213670808	18.515521866882427
5	26.183779083188956	30.467953301044204	24.354460225426234	18.993807390340606
6	27.109644827052943	34.92268763293491	22.711390322674635	15.256277217337514
7	83.08160214444044	5.91738256342214	6.842351802087513	4.158663490049913
8	84.77689347501104	4.949605201523162	6.396564592078928	3.8769367313868717
9	77.92086996864474	7.778751482035156	8.57484809840041	5.7255304509197025
10	39.29404698135494	30.702389410480595	15.776026106231383	14.227537501933089
11	29.46835000459459	25.685322192762065	25.789540922100425	19.056786880542923
12	28.098714187418892	23.708528229828072	28.245516913691205	19.94724066906183
13	24.771783395378964	24.776938300270967	29.767782740930173	20.6834955634199
14	20.738406506834732	28.107230986805686	29.184830235534324	21.96953227082526
15	20.00013447577979	26.6396519766819	32.58773984315641	20.772473704381895
16	23.174883510355755	26.018598000345154	30.42021439921825	20.38630409008084
17	23.094646295080203	25.533588687897403	27.91179285350881	23.459972163513584
18	23.77061121483178	26.06835403886798	28.726043700145905	21.434991046154327
19	24.789713499351155	26.928326650634165	26.894259453087006	21.387700396927677
20	26.51615838557344	26.657806206953744	27.641944788727347	19.184090618745476
21	25.152349852188706	26.71316540296788	27.491107789061292	20.643376955782124
22	24.062647783278834	25.503555763743986	26.833521225881206	23.600275227095974
23	23.60655076348624	26.43524879139893	27.590395739807295	22.367804705307535
24	22.892260246494104	25.234604204161126	29.32311616241985	22.55001938692492
25	22.912879866062124	27.25465454292803	26.949618649101144	22.882846941908706
26	22.045735212707065	28.083921851641836	27.374337986942404	22.496004948708695
27	22.292274142324683	27.51912357651784	27.650461588114133	22.538140693043342
28	20.980687036758955	27.91246523240777	28.542260134430954	22.564587596402326
29	21.76871510633672	25.635342027939583	29.196708929415905	23.39923393630779
30	22.90525957187394	25.909448492414445	28.054561306387377	23.130730629324237
31	24.086629297341638	26.939757091916437	26.35994235471573	22.613671256026198
32	23.765008057340474	26.81021209071736	27.044199947554446	22.38057990438772
33	22.999392617727942	26.820746026801025	26.90882766256441	23.271033692906627
34	22.928120454438485	26.120351340387337	28.029683287125962	22.92184491804822
35	21.901846128330238	25.960773415034836	28.411370375433965	23.726010081200958
36	22.339564791551336	27.367614197952832	27.403026153297905	22.889794857197927
37	23.611929794677895	27.19167505272571	27.67152946028146	21.524865692314936
38	21.931654926184002	27.322788938022352	27.868984730275205	22.876571405518437
39	23.159867048279047	25.17364185065568	28.5792409738736	23.087250127191673
40	23.78361054021162	25.995961244080263	28.59873996194335	21.621688253764763
41	20.878261317817817	26.691873404500903	28.555483586110448	23.874381691570832
42	22.244535240498724	26.398940330855243	28.480177149427245	22.876347279218788
43	22.822781093601865	25.491677069862405	28.6644089677415	23.021132868794222
44	22.371614852401624	25.940377921766473	27.95527335564137	23.732733870190533
45	21.91619021150799	25.29511830506727	27.46286787530509	25.325823608119645
46	22.390217335272773	26.695683551594996	27.338926031597328	23.575173081534906
47	21.93434444177983	25.913931018407492	29.160624595171868	22.9910999446408
48	22.002254710574505	25.027735629581983	28.94389446340802	24.026115196435498
49	21.832142849138346	24.67339194983157	29.747387247661806	23.747077953368283
50	20.7258554340542	25.40023353960424	29.23481040035681	24.639100625984756
51	21.6299809268519	24.44747263978197	28.202260537858294	25.72028589550784
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	120.0
1	368.0
2	616.0
3	1277.0
4	1938.0
5	1359.5
6	781.0
7	764.5
8	748.0
9	771.5
10	795.0
11	768.5
12	742.0
13	765.5
14	789.0
15	818.0
16	847.0
17	834.5
18	822.0
19	902.5
20	983.0
21	1095.0
22	1207.0
23	1499.5
24	1792.0
25	2204.0
26	3350.5
27	4085.0
28	5091.5
29	6098.0
30	7238.0
31	8378.0
32	9076.5
33	9775.0
34	11743.0
35	13711.0
36	15113.5
37	16516.0
38	17290.5
39	18065.0
40	20511.0
41	22957.0
42	24287.0
43	25617.0
44	27350.0
45	29083.0
46	31797.0
47	34511.0
48	36966.5
49	39422.0
50	38151.0
51	36880.0
52	33762.5
53	30645.0
54	28467.5
55	26290.0
56	24053.5
57	21817.0
58	21085.5
59	20354.0
60	19401.0
61	18448.0
62	17019.0
63	15590.0
64	13588.0
65	11586.0
66	9553.0
67	7520.0
68	6406.0
69	5292.0
70	4395.5
71	3499.0
72	2774.0
73	2049.0
74	1738.0
75	1180.0
76	933.0
77	647.5
78	362.0
79	295.5
80	229.0
81	193.0
82	157.0
83	109.0
84	61.0
85	37.0
86	13.0
87	8.0
88	3.0
89	3.0
90	3.0
91	2.5
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	446177.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.89482750159657
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.68483117958667	23.251653286319783
2	8.131362006076289	5.349595010858315
3	3.2755083590344145	3.2324184735142416
4	2.017131267029553	2.654127403080563
5	1.6227739284261018	2.669043422483242
6	1.4430823824688503	2.8481967625143505
7	1.3205172071983637	3.0406730020576167
8	1.1996696126057944	3.157033997245983
9	1.1326589336720305	3.353275821715538
>10	9.110971188702528	47.94398674435958
>50	0.04099595679964897	0.8675703364905337
>100	0.017764914613181224	1.0013020334366134
>500	0.0027330637866432653	0.6311237059236836
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	997	0.22345392075342296	No Hit
GCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTCTGC	648	0.1452338421747423	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	594	0.1331310219935138	No Hit
CCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTCTGC	569	0.1275278645022043	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.4825259930476023E-4	0.0	0.0	0.10870125533140436	0.0
2	4.4825259930476023E-4	0.0	0.0	0.5258002989844838	0.0
3	4.4825259930476023E-4	0.0	0.0	0.7743563652989732	0.0
4	4.4825259930476023E-4	0.0	0.0	1.0370323884915629	0.0
5	6.723788989571403E-4	0.0	0.0	1.8116128800901885	0.0
6	6.723788989571403E-4	0.0	0.0	2.365653092830872	0.0
7	6.723788989571403E-4	0.0	0.0	2.8854019817247414	0.0
8	6.723788989571403E-4	0.0	0.0	3.7895274745224428	0.0
9	6.723788989571403E-4	0.0	0.0	4.151491448461036	0.0
10	6.723788989571403E-4	0.0	0.0	4.826783989313658	0.0
11	6.723788989571403E-4	0.0	0.0	5.815853349679611	0.0
12	6.723788989571403E-4	0.0	0.0	6.565107569417518	0.0
13	6.723788989571403E-4	0.0	0.0	6.862074916456922	0.0
14	6.723788989571403E-4	0.0	0.0	6.988706275760516	0.0
15	6.723788989571403E-4	0.0	0.0	7.219332238102815	0.0
16	6.723788989571403E-4	0.0	0.0	7.696497130062733	0.0
17	6.723788989571403E-4	0.0	0.0	8.306344791416858	0.0
18	6.723788989571403E-4	0.0	0.0	8.953845671112585	0.0
19	6.723788989571403E-4	0.0	0.0	9.340015285413637	0.0
20	6.723788989571403E-4	0.0	0.0	9.72551252081573	0.0
21	6.723788989571403E-4	0.0	0.0	10.22822781093602	0.0
22	6.723788989571403E-4	0.0	0.0	10.799525748749936	0.0
23	6.723788989571403E-4	0.0	0.0	11.307395943762229	0.0
24	6.723788989571403E-4	0.0	0.0	11.741528586188888	0.0
25	6.723788989571403E-4	0.0	0.0	12.120974411500368	0.0
26	6.723788989571403E-4	0.0	0.0	12.456939734679287	0.0
27	6.723788989571403E-4	0.0	0.0	12.80747326733561	0.0
28	6.723788989571403E-4	0.0	0.0	13.151731263601665	0.0
29	6.723788989571403E-4	0.0	0.0	13.52691868921975	0.0
30	6.723788989571403E-4	0.0	0.0	13.974498909625552	0.0
31	8.965051986095205E-4	0.0	0.0	14.365599302518955	0.0
32	8.965051986095205E-4	0.0	0.0	14.726442644959288	0.0
33	8.965051986095205E-4	0.0	0.0	15.102750702075634	0.0
34	8.965051986095205E-4	0.0	0.0	15.476817496195457	0.0
35	8.965051986095205E-4	0.0	0.0	15.868142015388512	0.0
36	8.965051986095205E-4	0.0	0.0	16.252294492992693	0.0
37	8.965051986095205E-4	0.0	0.0	16.650791053774622	0.0
38	8.965051986095205E-4	0.0	0.0	17.03382289988054	0.0
39	0.0011206314982619005	0.0	0.0	17.45921461662076	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCTAG	35	1.2097007E-7	45.000004	1
TAGCGAG	20	7.028642E-4	45.0	1
GCGTTAG	20	7.028642E-4	45.0	1
GTATGCG	25	3.8869563E-5	45.0	1
TTAAGCG	20	7.028642E-4	45.0	1
AGCGCAT	20	7.028642E-4	45.0	27
GTATACG	40	6.7993824E-9	45.0	1
CGGTCTA	25	3.8869563E-5	45.0	31
CACAACG	40	6.7993824E-9	45.0	12
GTAACGG	55	1.8189894E-12	45.0	2
TTGACGG	30	2.1625056E-6	44.999996	2
AGTAACG	30	2.1625056E-6	44.999996	1
AACACGT	80	0.0	42.1875	41
TCGTCCC	75	0.0	42.0	38
TATACGG	65	0.0	41.53846	2
CTCGTCC	80	0.0	39.375	37
CCTCGTC	80	0.0	39.375	36
ACCGGGT	35	6.2396975E-6	38.57143	4
AGACACG	105	0.0	38.57143	24
TAACGGG	100	0.0	38.25	3
>>END_MODULE