##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550491_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	374725
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.318687037160586	33.0	31.0	34.0	30.0	34.0
2	32.43089732470478	34.0	31.0	34.0	31.0	34.0
3	32.51990926679565	34.0	31.0	34.0	31.0	34.0
4	36.00972179598372	37.0	35.0	37.0	35.0	37.0
5	35.964235105744216	37.0	35.0	37.0	35.0	37.0
6	35.9806978450864	37.0	35.0	37.0	35.0	37.0
7	36.119327506838346	37.0	35.0	37.0	35.0	37.0
8	36.10726265928348	37.0	35.0	37.0	35.0	37.0
9	37.67448395490026	39.0	38.0	39.0	35.0	39.0
10	37.4401814664087	39.0	37.0	39.0	35.0	39.0
11	37.38162118887184	39.0	37.0	39.0	35.0	39.0
12	37.238836480085396	39.0	37.0	39.0	34.0	39.0
13	37.179694442591234	39.0	37.0	39.0	34.0	39.0
14	38.39573820801922	40.0	38.0	41.0	34.0	41.0
15	38.46163186336647	40.0	38.0	41.0	34.0	41.0
16	38.491848689038626	40.0	38.0	41.0	34.0	41.0
17	38.49625992394422	40.0	38.0	41.0	34.0	41.0
18	38.434633397825074	40.0	38.0	41.0	34.0	41.0
19	38.389378877843754	40.0	37.0	41.0	34.0	41.0
20	38.27999733137634	40.0	37.0	41.0	34.0	41.0
21	38.2160864634065	40.0	37.0	41.0	34.0	41.0
22	38.20558276069117	40.0	37.0	41.0	34.0	41.0
23	38.169580358929885	40.0	37.0	41.0	34.0	41.0
24	38.13174728133965	40.0	37.0	41.0	34.0	41.0
25	38.09094669424245	40.0	37.0	41.0	34.0	41.0
26	37.95608246047101	40.0	36.0	41.0	34.0	41.0
27	37.89185936353326	40.0	36.0	41.0	33.0	41.0
28	37.806546133831475	40.0	36.0	41.0	33.0	41.0
29	37.74477283341117	40.0	36.0	41.0	33.0	41.0
30	37.7276456067783	40.0	36.0	41.0	33.0	41.0
31	37.63149776502769	40.0	36.0	41.0	33.0	41.0
32	37.44877710320902	40.0	35.0	41.0	33.0	41.0
33	37.338885849623054	40.0	35.0	41.0	32.0	41.0
34	37.170818600306895	40.0	35.0	41.0	32.0	41.0
35	37.11906331309627	40.0	35.0	41.0	31.0	41.0
36	36.99583160984722	39.0	35.0	41.0	31.0	41.0
37	36.94294482620588	39.0	35.0	41.0	31.0	41.0
38	36.82407899126026	39.0	35.0	41.0	31.0	41.0
39	36.73951297618253	39.0	35.0	41.0	31.0	41.0
40	36.68343985589432	39.0	35.0	41.0	31.0	41.0
41	36.572232970845285	39.0	35.0	41.0	31.0	41.0
42	36.50982720661818	39.0	35.0	41.0	31.0	41.0
43	36.42245113082927	39.0	35.0	41.0	30.0	41.0
44	36.231300286877044	39.0	35.0	41.0	30.0	41.0
45	36.13404229768497	38.0	35.0	41.0	30.0	41.0
46	36.0437414103676	38.0	35.0	40.0	30.0	41.0
47	35.95401961438388	38.0	35.0	40.0	29.0	41.0
48	35.8166308626326	38.0	35.0	40.0	29.0	41.0
49	35.71666688905197	38.0	35.0	40.0	29.0	41.0
50	35.61337514177063	38.0	35.0	40.0	29.0	41.0
51	34.408638334778836	36.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	3.0
12	4.0
13	3.0
14	13.0
15	10.0
16	31.0
17	56.0
18	87.0
19	180.0
20	276.0
21	459.0
22	691.0
23	1011.0
24	1548.0
25	2102.0
26	2899.0
27	3557.0
28	4118.0
29	4787.0
30	5662.0
31	7161.0
32	9021.0
33	12474.0
34	22353.0
35	29621.0
36	28166.0
37	41597.0
38	71861.0
39	124882.0
40	88.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.94702782040163	21.64974314497298	25.06878377476816	11.33444525985723
2	32.192941490426314	24.598839148709054	25.280138768430184	17.928080592434455
3	32.450730535726194	24.612182266995795	26.21949429581693	16.71759290146107
4	28.42511174861565	26.213089599039296	25.727666955767564	19.63413169657749
5	25.781573153646008	30.671025418640337	24.123557275335244	19.42384415237841
6	26.937354059643738	34.745213156314634	23.103075588765094	15.214357195276538
7	81.46800987390753	6.414303822803389	7.524718126626192	4.592968176662886
8	82.99099339515644	5.308159316832343	7.400893988925211	4.299953299085996
9	76.23750750550403	7.8126626192541195	9.688705050370272	6.2611248248715725
10	41.12055507372073	26.807392087530857	16.538261391687236	15.53379144706118
11	32.16091800653813	25.27346720928681	23.188471545800255	19.37714323837481
12	28.27219961304957	23.599439589031956	27.214624057642272	20.913736740276203
13	25.71325638801788	24.680499032623924	28.572152912135568	21.03409166722263
14	22.069517646273933	27.290412969510975	28.632196944425914	22.00787243978918
15	21.28387484155047	25.33591300286877	31.77663619987991	21.603575955700848
16	24.855827606911735	24.660751217559543	29.355927680298887	21.127493495229835
17	24.31596504103009	25.16058442858096	27.627460137434117	22.895990392954836
18	25.424778170658485	24.51691240242845	28.144372539862566	21.913936887050504
19	25.775168456868368	26.50290212822737	25.746080459003267	21.975848955900997
20	27.739542331042767	25.77063179665088	25.680699179398225	20.809126692908134
21	25.44932950830609	26.982720661818664	26.246447394756157	21.32150243511909
22	24.98819134031623	24.687170591767295	26.162652611915405	24.161985456001066
23	24.484355193808792	26.36520114750817	26.71852691974114	22.43191673894189
24	24.48889185402629	24.827006471412368	28.280739208753086	22.40336246580826
25	24.037894455934353	26.31476416038428	26.318767095870303	23.328574287811062
26	23.41717259323504	27.569551004069652	26.16665554740143	22.84662085529388
27	24.104876909733804	26.325171792647943	26.52131563146307	23.04863566615518
28	22.34038294749483	27.108679698445528	27.304289812529188	23.246647541530454
29	23.451330976049103	26.19414237107212	26.521849356194544	23.832677296684235
30	24.7109213423177	24.849156047768364	27.040362932817395	23.399559677096537
31	25.70071385682834	25.638534925612117	25.420508372806726	23.240242844752817
32	25.40209486957102	25.76903062245647	25.592901461071456	23.23597304690106
33	24.997531523116955	25.488558276069117	26.428180665821603	23.08572953499233
34	23.298151978117286	25.407965841617187	28.536393355127093	22.757488825138434
35	24.077390086063115	24.60897991860698	28.022149576355993	23.291480418973915
36	24.314363866835677	27.234638735072387	26.52932150243512	21.921675895656815
37	24.650076722930148	26.974981653212353	27.463339782507173	20.911601841350326
38	23.79077990526386	26.52398425512042	27.415304556674897	22.26993128294082
39	23.921542464473948	24.655413970244847	28.687704316498763	22.73533924878244
40	24.769364200413637	24.085129094669426	29.12429114684102	22.02121555807592
41	22.301687904463275	25.279605043698712	27.99172726666222	24.426979785175796
42	23.878043898859165	25.305490693174992	27.34618720394956	23.470278204016278
43	24.329841884048303	24.369604376542796	28.08219360864634	23.21836013076256
44	23.976249249449598	24.894789512309025	27.31576489425579	23.81319634398559
45	23.42651277603576	24.472346387350726	27.57168590299553	24.529454933617988
46	23.959170058042563	25.188338114617387	27.076923076923077	23.775568750416973
47	22.989125358596304	25.642804723463875	28.63433184335179	22.733738074588032
48	23.33337781039429	24.458469544332512	28.5892321035426	23.618920541730603
49	23.704316498765763	24.364533991593834	28.920675161785308	23.010474347855094
50	22.004403229034626	24.087530855961038	29.624924944959634	24.283140970044702
51	22.28781106144506	24.018146640869972	27.849756488091266	25.844285809593703
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	161.0
1	363.5
2	566.0
3	1277.5
4	1989.0
5	1363.5
6	738.0
7	731.0
8	724.0
9	745.0
10	766.0
11	770.5
12	775.0
13	743.5
14	712.0
15	727.5
16	743.0
17	707.0
18	671.0
19	710.5
20	750.0
21	837.5
22	925.0
23	1113.5
24	1302.0
25	1579.5
26	2138.0
27	2419.0
28	3240.5
29	4062.0
30	4821.5
31	5581.0
32	6310.0
33	7039.0
34	7946.5
35	8854.0
36	9907.0
37	10960.0
38	12020.0
39	13080.0
40	14340.0
41	15600.0
42	17432.0
43	19264.0
44	21124.0
45	22984.0
46	25319.5
47	27655.0
48	29689.0
49	31723.0
50	31939.0
51	32155.0
52	30227.5
53	28300.0
54	25885.0
55	23470.0
56	22163.0
57	20856.0
58	20155.0
59	19454.0
60	18568.5
61	17683.0
62	16313.0
63	14943.0
64	13205.0
65	11467.0
66	9742.0
67	8017.0
68	6752.0
69	5487.0
70	4548.0
71	3609.0
72	3213.5
73	2818.0
74	2358.0
75	1635.5
76	1373.0
77	1043.5
78	714.0
79	517.5
80	321.0
81	234.0
82	147.0
83	109.0
84	71.0
85	45.5
86	20.0
87	17.0
88	14.0
89	10.0
90	6.0
91	3.0
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	374725.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.54408361561989
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.81981612702747	24.8168138244627
2	8.159658386273158	5.800551599131742
3	3.6103032684546497	3.849747637550935
4	2.5266720632313877	3.59232972338989
5	2.0971462459028736	3.727057075927754
6	1.7524490433514843	3.7373517197398933
7	1.5910180392186395	3.958589475396285
8	1.422553525526584	4.045068916720941
9	1.3455888897765578	4.304495160942039
>10	7.6235941888651695	39.81796039108658
>50	0.029364833419259977	0.6888724223125311
>100	0.02032950005948768	1.2265354745370356
>500	7.529444466476917E-4	0.14880072525476584
>1k	7.529444466476917E-4	0.2858258535469243
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1068	0.2850090066048435	No Hit
GCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGC	556	0.14837547534858897	No Hit
CCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGC	411	0.10968043231703249	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.668623657348722E-4	0.0	0.0	0.10647808392821402	0.0
2	2.668623657348722E-4	0.0	0.0	0.4835546067115885	0.0
3	2.668623657348722E-4	0.0	0.0	0.7106544799519647	0.0
4	2.668623657348722E-4	0.0	0.0	0.9542998198679031	0.0
5	2.668623657348722E-4	0.0	0.0	1.7193942224297818	0.0
6	2.668623657348722E-4	0.0	0.0	2.4089665754886918	0.0
7	2.668623657348722E-4	0.0	0.0	3.009673760757889	0.0
8	2.668623657348722E-4	0.0	0.0	3.993862165588098	0.0
9	2.668623657348722E-4	0.0	0.0	4.496630862632597	0.0
10	2.668623657348722E-4	0.0	0.0	5.328974581359664	0.0
11	2.668623657348722E-4	0.0	0.0	6.326239242110882	0.0
12	2.668623657348722E-4	0.0	0.0	7.080125425311896	0.0
13	2.668623657348722E-4	0.0	0.0	7.423043565281207	0.0
14	2.668623657348722E-4	0.0	0.0	7.573020214824204	0.0
15	2.668623657348722E-4	0.0	0.0	7.808659683768097	0.0
16	2.668623657348722E-4	0.0	0.0	8.2311028087264	0.0
17	2.668623657348722E-4	0.0	0.0	8.795516712255655	0.0
18	2.668623657348722E-4	0.0	0.0	9.371672559877243	0.0
19	2.668623657348722E-4	0.0	0.0	9.712989525652144	0.0
20	5.337247314697444E-4	0.0	0.0	10.063646674227767	0.0
21	5.337247314697444E-4	0.0	0.0	10.497831743278404	0.0
22	5.337247314697444E-4	0.0	0.0	10.94402561878711	0.0
23	5.337247314697444E-4	0.0	0.0	11.42544532657282	0.0
24	5.337247314697444E-4	0.0	0.0	11.813996931082794	0.0
25	5.337247314697444E-4	0.0	0.0	12.136633531256255	0.0
26	5.337247314697444E-4	0.0	0.0	12.457402094869572	0.0
27	5.337247314697444E-4	0.0	0.0	12.766962439122024	0.0
28	8.005870972046167E-4	0.0	0.0	13.106678230702515	0.0
29	8.005870972046167E-4	0.0	0.0	13.468276736273268	0.0
30	8.005870972046167E-4	0.0	0.0	13.90886650210154	0.0
31	8.005870972046167E-4	0.0	0.0	14.270731870038027	0.0
32	0.0010674494629394889	0.0	0.0	14.625391954099673	0.0
33	0.0010674494629394889	0.0	0.0	14.991260257522184	0.0
34	0.0010674494629394889	0.0	0.0	15.348322102875443	0.0
35	0.0010674494629394889	0.0	0.0	15.731269597704983	0.0
36	0.0010674494629394889	0.0	0.0	16.081126159183402	0.0
37	0.0010674494629394889	0.0	0.0	16.43765427980519	0.0
38	0.0013343118286743613	0.0	0.0	16.80779238107946	0.0
39	0.0013343118286743613	0.0	0.0	17.205684168390153	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGTACG	20	7.027193E-4	45.000004	1
CTCGGCA	20	7.027193E-4	45.000004	26
ACTACGG	20	7.027193E-4	45.000004	2
TGTTACG	20	7.027193E-4	45.000004	1
ACGTAAG	20	7.027193E-4	45.000004	1
TGGTCGC	20	7.027193E-4	45.000004	34
ACAACGG	25	3.8857565E-5	45.0	2
TATTGCG	35	1.2089913E-7	45.0	1
TGCGTAG	25	3.8857565E-5	45.0	1
ACAACGA	75	0.0	42.0	13
ACGGGCG	115	0.0	41.08696	5
CGTTCGG	40	3.4499135E-7	39.375004	45
GTAAGCG	40	3.4499135E-7	39.375004	1
CACGTGA	200	0.0	39.375	43
AACACGT	200	0.0	39.375	41
GCGAGAC	220	0.0	38.863632	21
GACGGGC	110	0.0	38.863632	4
TCAAGCG	220	0.0	38.863632	17
ACACGAC	215	0.0	38.720932	26
GACACGA	215	0.0	38.720932	25
>>END_MODULE