SRR3550490_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550490_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	177757
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGC	480	0.2700315599385678	TruSeq Adapter, Index 20 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGC	434	0.2441535354444551	TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGCT	382	0.21490011645111023	TruSeq Adapter, Index 27 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	358	0.20139853845418185	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	318	0.17889590845930117	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	282	0.15864354146390858	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	250	0.1406414374680041	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGC	236	0.13276551696979585	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	212	0.11926393897286745	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTC	204	0.11476341297389132	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCG	195	0.10970032122504317	No Hit
TCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGC	179	0.10069926922709092	TruSeq Adapter, Index 20 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	30	2.1551205E-6	45.000004	6
CCTTATC	30	2.1551205E-6	45.000004	43
ACGATGG	30	2.1551205E-6	45.000004	2
TAGGGTA	30	2.1551205E-6	45.000004	5
CGGGTAC	20	7.0171856E-4	45.0	6
TTGGCAG	20	7.0171856E-4	45.0	1
TACCAAT	25	3.877466E-5	45.0	27
GCGAACG	20	7.0171856E-4	45.0	26
TTGCTCA	20	7.0171856E-4	45.0	36
CCAATGT	25	3.877466E-5	45.0	29
AGGGTAC	35	1.2041892E-7	45.0	6
ATGTCGA	20	7.0171856E-4	45.0	29
ACGGGCG	25	3.877466E-5	45.0	5
ATGTACC	25	3.877466E-5	45.0	24
TCGGACC	20	7.0171856E-4	45.0	29
AATGATC	25	3.877466E-5	45.0	10
ACGGCCG	20	7.0171856E-4	45.0	30
TGCGAGC	20	7.0171856E-4	45.0	21
TGTTCGG	20	7.0171856E-4	45.0	1
GCGGGTA	40	6.7593646E-9	45.0	5