##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550490_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	177757
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.239112946325605	33.0	31.0	34.0	30.0	34.0
2	32.3340684192465	34.0	31.0	34.0	30.0	34.0
3	32.443678729951564	34.0	31.0	34.0	30.0	34.0
4	35.942027599475686	37.0	35.0	37.0	35.0	37.0
5	35.9076773347885	37.0	35.0	37.0	35.0	37.0
6	35.92282160477506	37.0	35.0	37.0	35.0	37.0
7	36.07202529295611	37.0	35.0	37.0	35.0	37.0
8	36.04102792013816	37.0	35.0	37.0	35.0	37.0
9	37.55432978729389	39.0	38.0	39.0	35.0	39.0
10	37.395056172190124	39.0	37.0	39.0	35.0	39.0
11	37.29025579864647	39.0	37.0	39.0	34.0	39.0
12	37.03091298795547	39.0	35.0	39.0	34.0	39.0
13	36.843156668935684	39.0	35.0	39.0	33.0	39.0
14	37.967241796384954	40.0	36.0	41.0	33.0	41.0
15	38.092677081633916	40.0	36.0	41.0	33.0	41.0
16	38.16691325798703	40.0	36.0	41.0	34.0	41.0
17	38.182175666781056	40.0	36.0	41.0	34.0	41.0
18	38.14229538077263	40.0	36.0	41.0	34.0	41.0
19	38.08604443144292	40.0	36.0	41.0	34.0	41.0
20	37.964811512345506	40.0	36.0	41.0	34.0	41.0
21	37.902085431234774	40.0	35.0	41.0	34.0	41.0
22	37.872533852394	40.0	35.0	41.0	34.0	41.0
23	37.82751171543174	40.0	35.0	41.0	33.0	41.0
24	37.777792154458055	40.0	35.0	41.0	33.0	41.0
25	37.741197252428876	40.0	35.0	41.0	33.0	41.0
26	37.61509813959506	40.0	35.0	41.0	33.0	41.0
27	37.53700276219783	39.0	35.0	41.0	33.0	41.0
28	37.44151285181456	39.0	35.0	41.0	33.0	41.0
29	37.4180369830724	39.0	35.0	41.0	33.0	41.0
30	37.3771440787142	39.0	35.0	41.0	33.0	41.0
31	37.27420017214512	39.0	35.0	41.0	33.0	41.0
32	37.11656362337348	39.0	35.0	41.0	32.0	41.0
33	36.96887323705958	39.0	35.0	41.0	32.0	41.0
34	36.82825430222157	39.0	35.0	41.0	31.0	41.0
35	36.76566886254831	39.0	35.0	41.0	31.0	41.0
36	36.59628031526184	39.0	35.0	41.0	31.0	41.0
37	36.52156033236385	39.0	35.0	41.0	31.0	41.0
38	36.42102983286171	39.0	35.0	41.0	31.0	41.0
39	36.308145389492395	38.0	35.0	41.0	30.0	41.0
40	36.180313574148975	38.0	35.0	41.0	30.0	41.0
41	36.09468544136096	38.0	35.0	40.0	30.0	41.0
42	36.022215721462445	38.0	35.0	40.0	30.0	41.0
43	35.94395719999775	38.0	35.0	40.0	30.0	41.0
44	35.69971927969081	38.0	35.0	40.0	29.0	41.0
45	35.64671996039537	38.0	35.0	40.0	29.0	41.0
46	35.56018609675006	38.0	35.0	40.0	29.0	41.0
47	35.44907936115033	37.0	35.0	40.0	28.0	41.0
48	35.337860112400634	37.0	34.0	40.0	28.0	41.0
49	35.254662263652065	37.0	34.0	40.0	28.0	41.0
50	35.127843066658414	37.0	34.0	40.0	27.0	41.0
51	33.85152764729378	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	0.0
13	4.0
14	4.0
15	7.0
16	13.0
17	30.0
18	46.0
19	92.0
20	162.0
21	257.0
22	370.0
23	557.0
24	865.0
25	1196.0
26	1496.0
27	1894.0
28	2144.0
29	2441.0
30	2954.0
31	3642.0
32	4862.0
33	6601.0
34	12851.0
35	18708.0
36	13608.0
37	19466.0
38	31553.0
39	51896.0
40	36.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.13737855611875	23.025816142261625	25.318271572990092	11.518533728629533
2	29.89868190844805	27.211305321309425	26.72637364491975	16.163639125322774
3	31.68370303279196	25.38127893697576	26.432151757736687	16.5028662724956
4	28.995763879903464	27.128608156078243	25.22544822426121	18.650179739757082
5	26.40852399624206	31.266841812136793	23.092198900746524	19.232435290874623
6	26.2650697300247	35.870879909089375	22.279853957931333	15.584196402954595
7	79.81401576309231	7.124895222129086	8.221335868629646	4.839753146148956
8	80.52622400243028	6.35586784205404	7.641330580511598	5.476577575004079
9	73.50315318102804	9.26320763739262	10.248260265418521	6.985378916160827
10	45.192594384468684	24.581310440657752	15.661267910687062	14.564827264186503
11	35.13279364525729	23.100074821244736	21.823050569035257	19.94408096446272
12	30.168713468386617	22.091956997474078	26.466468268478877	21.272861265660424
13	24.20607908549312	27.137046642326325	29.118403213375565	19.538471058804998
14	19.955332279460162	30.363361217842332	28.00114763412974	21.680158868567766
15	19.98233543545402	24.998171661312917	34.87907649206501	20.140416411168054
16	23.016815090263677	23.787530167588336	31.306783980377705	21.88887076177028
17	22.040763514235724	24.13688349825886	27.25293518679996	26.56941780070546
18	23.739149513099346	24.71238826037793	29.045269665892203	22.503192560630524
19	26.08673638731527	25.9674724483424	25.275516575999816	22.67027458834251
20	26.510348396968897	26.142430396552598	25.47072689120541	21.8764943152731
21	24.507614327424516	27.22480689930636	26.730311605168854	21.53726716810027
22	23.5630664333894	25.311520783991632	25.414470316218207	25.71094246640076
23	23.477556439408858	26.022041326079986	25.664812074911254	24.835590159599903
24	22.927929701783896	26.083923558565907	28.364565108547062	22.623581631103136
25	22.457624734890892	27.03859763609872	26.445090769983743	24.058686859026647
26	21.320116788649674	28.255427353071887	26.230753219282505	24.19370263899593
27	23.1490180414836	26.78263021990695	26.970527180364208	23.097824558245243
28	20.05490641718751	26.584044510202133	29.423876415556066	23.937172657054294
29	21.761730902299206	25.172004478023368	28.21379748758136	24.852467132096066
30	22.91836608403607	26.00741461658331	27.75080587543669	23.323413423943922
31	25.25638934050417	25.328397756487792	24.33265637921432	25.08255652379372
32	25.87577423111326	25.711505032150633	23.88035351631722	24.532367220418884
33	24.308466051969823	26.127241121306056	25.99447560433626	23.56981722238787
34	22.030637330738028	24.25727256873147	27.301315841288954	26.41077425924155
35	23.290222044701476	23.515810910400152	28.48326648177006	24.710700563128317
36	25.458350444708227	24.49748814392682	27.309754327537032	22.734407083827925
37	23.81959641533104	25.092120141541542	29.492509437040454	21.595774006086963
38	23.480369268158217	26.133429344554642	26.589107601950978	23.797093785336163
39	25.458913010458094	23.68233037236227	28.172167622090832	22.6865889950888
40	23.547877158142857	23.556878210140813	29.82554836096469	23.069696270751646
41	21.617151504582097	23.687393464111118	27.2259320308061	27.469523000500683
42	23.11638922799102	22.77266155481922	28.537272793757772	25.57367642343199
43	25.069054945796793	21.22391804542156	28.616594564489723	25.090432444291928
44	22.977998053522505	24.072188437023577	26.975027706363182	25.97478580309074
45	21.515889669605134	25.525295768942996	27.257998278548808	25.700816282903066
46	24.2848382904752	25.031363040555366	27.8149383709221	22.868860298047334
47	23.02919153676086	23.264906585957235	29.65284067575398	24.053061201527928
48	22.75634714807293	22.122898113717042	29.76422869422864	25.356526043981393
49	23.083197848748572	21.86017990852681	30.66039593377476	24.39622630894986
50	22.08126824822651	22.548760386370155	29.777167706475694	25.592803658927636
51	22.408118948902153	23.569254656637995	27.037472504598973	26.98515388986088
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	109.0
1	238.0
2	367.0
3	560.5
4	754.0
5	537.0
6	320.0
7	344.0
8	368.0
9	358.5
10	349.0
11	390.0
12	431.0
13	448.5
14	466.0
15	450.5
16	435.0
17	410.5
18	386.0
19	384.0
20	382.0
21	443.5
22	505.0
23	576.0
24	647.0
25	681.5
26	1064.5
27	1413.0
28	1641.5
29	1870.0
30	2271.5
31	2673.0
32	2767.0
33	2861.0
34	2974.0
35	3087.0
36	3312.0
37	3537.0
38	4077.5
39	4618.0
40	5435.0
41	6252.0
42	8164.5
43	10077.0
44	11386.5
45	12696.0
46	14360.0
47	16024.0
48	17110.0
49	18196.0
50	17418.0
51	16640.0
52	14455.0
53	12270.0
54	11100.5
55	9931.0
56	9465.5
57	9000.0
58	8474.0
59	7948.0
60	7719.5
61	7491.0
62	7133.0
63	6775.0
64	5927.0
65	5079.0
66	4726.5
67	4374.0
68	3658.5
69	2943.0
70	2504.5
71	2066.0
72	1815.5
73	1565.0
74	1271.0
75	754.0
76	531.0
77	471.0
78	411.0
79	280.0
80	149.0
81	91.5
82	34.0
83	26.5
84	19.0
85	14.0
86	9.0
87	6.0
88	3.0
89	2.0
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	177757.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.07034884702149
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.2139878087905	26.027104417828834
2	8.19377606673083	5.747171700692518
3	2.9371190247032404	3.0901736640469855
4	1.7420596727622715	2.4437856174440387
5	1.195059351940969	2.095557418273261
6	1.0699390439525185	2.251388130987809
7	0.9640680141161373	2.3667141097115723
8	0.8325312800769971	2.335772993468612
9	0.8036573628488931	2.5366089661729214
>10	7.95636830285531	47.18182687601614
>50	0.05935194096888033	1.3687224694386155
>100	0.03208213025344883	2.555173635918698
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGC	480	0.2700315599385678	TruSeq Adapter, Index 20 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGC	434	0.2441535354444551	TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGCT	382	0.21490011645111023	TruSeq Adapter, Index 27 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	358	0.20139853845418185	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	318	0.17889590845930117	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	282	0.15864354146390858	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	250	0.1406414374680041	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGC	236	0.13276551696979585	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	212	0.11926393897286745	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTC	204	0.11476341297389132	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCG	195	0.10970032122504317	No Hit
TCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGC	179	0.10069926922709092	TruSeq Adapter, Index 20 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.24471610119432707	0.0
2	0.0	0.0	0.0	1.0289327565159179	0.0
3	0.0	0.0	0.0	1.412602597928633	0.0
4	0.0	0.0	0.0	1.8710936840743262	0.0
5	0.0	0.0	0.0	3.283696282002959	0.0
6	0.0	0.0	0.0	4.106729974065719	0.0
7	0.0	0.0	0.0	4.960704782371439	0.0
8	0.0	0.0	0.0	6.459379940030491	0.0
9	0.0	0.0	0.0	7.060762726643676	0.0
10	0.0	0.0	0.0	8.298969942111984	0.0
11	0.0	0.0	0.0	9.705384316792026	0.0
12	0.0	0.0	0.0	10.957093110257262	0.0
13	0.0	0.0	0.0	11.486467480886828	0.0
14	0.0	0.0	0.0	11.67380187559421	0.0
15	0.0	0.0	0.0	12.041719876010509	0.0
16	0.0	0.0	0.0	12.711173118358207	0.0
17	0.0	0.0	0.0	13.49876516817903	0.0
18	0.0	0.0	0.0	14.380305698228481	0.0
19	0.0	0.0	0.0	14.881551781364447	0.0
20	0.0	0.0	0.0	15.362545497505021	0.0
21	0.0	0.0	0.0	15.93411229937499	0.0
22	0.0	0.0	0.0	16.48936469449867	0.0
23	0.0	0.0	0.0	17.05586840461979	0.0
24	0.0	0.0	0.0	17.52448567426318	0.0
25	5.625657498720163E-4	0.0	0.0	17.898029332178197	0.0
26	5.625657498720163E-4	0.0	0.0	18.263697069595008	0.0
27	5.625657498720163E-4	0.0	0.0	18.63274020151105	0.0
28	5.625657498720163E-4	0.0	0.0	19.000095636177477	0.0
29	0.0011251314997440325	0.0	0.0	19.38826600358917	0.0
30	0.0011251314997440325	0.0	0.0	19.825379591239727	0.0
31	0.0011251314997440325	0.0	0.0	20.220300747649883	0.0
32	0.0011251314997440325	0.0	0.0	20.60453315481247	0.0
33	0.0011251314997440325	0.0	0.0	20.976389115477872	0.0
34	0.0011251314997440325	0.0	0.0	21.362309219890076	0.0
35	0.0011251314997440325	0.0	0.0	21.764543731048565	0.0
36	0.0011251314997440325	0.0	0.0	22.155526927209618	0.0
37	0.0011251314997440325	0.0	0.0	22.533571111123614	0.0
38	0.0011251314997440325	0.0	0.0	22.88461213904375	0.0
39	0.0011251314997440325	0.0	0.0	23.236778298463634	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	30	2.1551205E-6	45.000004	6
CCTTATC	30	2.1551205E-6	45.000004	43
ACGATGG	30	2.1551205E-6	45.000004	2
TAGGGTA	30	2.1551205E-6	45.000004	5
CGGGTAC	20	7.0171856E-4	45.0	6
TTGGCAG	20	7.0171856E-4	45.0	1
TACCAAT	25	3.877466E-5	45.0	27
GCGAACG	20	7.0171856E-4	45.0	26
TTGCTCA	20	7.0171856E-4	45.0	36
CCAATGT	25	3.877466E-5	45.0	29
AGGGTAC	35	1.2041892E-7	45.0	6
ATGTCGA	20	7.0171856E-4	45.0	29
ACGGGCG	25	3.877466E-5	45.0	5
ATGTACC	25	3.877466E-5	45.0	24
TCGGACC	20	7.0171856E-4	45.0	29
AATGATC	25	3.877466E-5	45.0	10
ACGGCCG	20	7.0171856E-4	45.0	30
TGCGAGC	20	7.0171856E-4	45.0	21
TGTTCGG	20	7.0171856E-4	45.0	1
GCGGGTA	40	6.7593646E-9	45.0	5
>>END_MODULE