FastQCFastQC Report
Sat 18 Jun 2016
SRR3550488_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550488_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences591763
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47450.8018412776736632No Hit
GCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTCTGC12380.20920537444889253No Hit
CTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTCTGCT10040.16966251691978038Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTCTGC9700.16391697351811452No Hit
CGCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTCTG8870.1498910881552243No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTT6680.1128830291856706No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG207.030509E-445.0000041
ACGGGTA207.030509E-445.0000045
CGCAGTA207.030509E-445.00000437
CCGAATT207.030509E-445.00000418
CGGTCTA207.030509E-445.00000431
GTACGTT207.030509E-445.00000433
TCGTTTA253.888504E-545.038
TAATACG253.888504E-545.01
TAACGCC253.888504E-545.012
CATCGTT253.888504E-545.036
GCTACGA351.210592E-745.010
TAGTACG253.888504E-545.01
TATAGCG302.1637097E-644.9999961
CGCGACC650.041.5384610
CGTTTTT25600.041.396491
GTAAGCG501.0804797E-940.51
TTGCGAG451.9255822E-840.01
AATACGG451.9255822E-840.02
TAGGGTA1750.038.5714265
ATAACGC356.2431536E-638.57142611
Produced by FastQC (version 0.11.5)