##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550488_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	591763
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.269800578947994	33.0	31.0	34.0	30.0	34.0
2	32.38308410630607	34.0	31.0	34.0	30.0	34.0
3	32.453668106995536	34.0	31.0	34.0	30.0	34.0
4	35.977541684762315	37.0	35.0	37.0	35.0	37.0
5	35.95435165767376	37.0	35.0	37.0	35.0	37.0
6	35.980742290410184	37.0	35.0	37.0	35.0	37.0
7	36.14351353497938	37.0	35.0	37.0	35.0	37.0
8	36.13046777172618	37.0	36.0	37.0	35.0	37.0
9	37.701617032494426	39.0	38.0	39.0	35.0	39.0
10	37.49732916725108	39.0	37.0	39.0	35.0	39.0
11	37.45327605815166	39.0	37.0	39.0	35.0	39.0
12	37.399540018554724	39.0	37.0	39.0	35.0	39.0
13	37.38405408922153	39.0	37.0	39.0	35.0	39.0
14	38.623011239296815	40.0	38.0	41.0	35.0	41.0
15	38.67779330576599	40.0	38.0	41.0	35.0	41.0
16	38.67492898339369	40.0	38.0	41.0	35.0	41.0
17	38.6871315036594	40.0	38.0	41.0	35.0	41.0
18	38.59528730251807	40.0	38.0	41.0	35.0	41.0
19	38.509628009862055	40.0	37.0	41.0	34.0	41.0
20	38.34838440389142	40.0	37.0	41.0	34.0	41.0
21	38.30637603229671	40.0	37.0	41.0	34.0	41.0
22	38.282873380052486	40.0	37.0	41.0	34.0	41.0
23	38.24709554331717	40.0	37.0	41.0	34.0	41.0
24	38.225458502812785	40.0	37.0	41.0	34.0	41.0
25	38.170010629255295	40.0	37.0	41.0	34.0	41.0
26	38.040776459494765	40.0	37.0	41.0	34.0	41.0
27	37.989362295378385	40.0	36.0	41.0	34.0	41.0
28	37.90158053139517	40.0	36.0	41.0	33.0	41.0
29	37.829294835939386	40.0	36.0	41.0	33.0	41.0
30	37.77986964375941	40.0	36.0	41.0	33.0	41.0
31	37.6443288951827	40.0	36.0	41.0	33.0	41.0
32	37.42183272695319	40.0	36.0	41.0	33.0	41.0
33	37.212505682173436	40.0	36.0	41.0	32.0	41.0
34	37.00216133823845	40.0	35.0	41.0	31.0	41.0
35	36.90944347652692	40.0	35.0	41.0	31.0	41.0
36	36.74823197800471	39.0	35.0	41.0	31.0	41.0
37	36.7221877677381	39.0	35.0	41.0	31.0	41.0
38	36.606712484558855	39.0	35.0	41.0	30.0	41.0
39	36.520027443419075	39.0	35.0	41.0	30.0	41.0
40	36.43986528390589	39.0	35.0	41.0	30.0	41.0
41	36.333868457473685	39.0	35.0	41.0	30.0	41.0
42	36.26361060086555	39.0	35.0	41.0	30.0	41.0
43	36.18556922281386	39.0	35.0	41.0	30.0	41.0
44	35.97322069815112	39.0	35.0	41.0	29.0	41.0
45	35.893170069774555	39.0	35.0	41.0	28.0	41.0
46	35.805746557321086	39.0	35.0	41.0	28.0	41.0
47	35.71635097158829	38.0	35.0	40.0	28.0	41.0
48	35.588142550311524	38.0	35.0	40.0	27.0	41.0
49	35.50468853240233	38.0	35.0	40.0	27.0	41.0
50	35.42402786250577	38.0	35.0	40.0	27.0	41.0
51	34.20579353558772	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	6.0
11	7.0
12	3.0
13	6.0
14	8.0
15	13.0
16	24.0
17	54.0
18	104.0
19	223.0
20	392.0
21	696.0
22	1055.0
23	1657.0
24	2821.0
25	4393.0
26	6290.0
27	7254.0
28	7730.0
29	8214.0
30	9354.0
31	11211.0
32	13936.0
33	18564.0
34	30954.0
35	42125.0
36	43234.0
37	62825.0
38	113611.0
39	204837.0
40	160.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.13589393050934	21.123828289365846	24.465199750575824	13.275078029548993
2	34.77152170716993	23.566022208215113	24.59650231596095	17.065953768654005
3	31.282962942934926	23.399739422708077	29.093065974047043	16.224231660309954
4	27.699264739431158	25.84075043556289	27.97674068841749	18.483244136588464
5	25.04448571472025	30.196548280308168	26.317968511042427	18.440997493929157
6	26.204071562432933	33.67395393088111	25.567499150842483	14.554475355843472
7	79.61852971544351	5.606467454031428	10.274214508173035	4.500788322352022
8	80.43084816049668	4.860222758097414	10.168090941812855	4.540838139593046
9	72.99898777044189	8.149546355551124	12.852611602955912	5.99885427105108
10	35.78679302355842	31.78079738003221	18.632290291890506	13.800119304518871
11	27.131807835231335	23.774382649810818	29.925493821006043	19.1683156939518
12	24.32663076265329	23.221796563827073	32.2228662488192	20.22870642470043
13	23.281617809832653	23.25559387795452	33.81962035477041	19.643167957442422
14	20.569721324246363	26.413952883164377	31.89537027492425	21.12095551766501
15	20.29038652298302	25.647936758465807	34.476302168266685	19.58537455028449
16	23.609451756868882	25.009843467739618	31.946404219256696	19.434300556134804
17	23.17802904203203	24.944445664903007	29.729469399066854	22.1480558939981
18	23.436916468248267	24.135844924403855	31.699852812696978	20.7273857946509
19	23.798378742841305	25.52018291106406	29.501675501847867	21.17976284424677
20	24.369215378453873	25.755412217391083	29.388623486091557	20.486748918063483
21	23.612324528569715	26.521428342089653	30.067780513482596	19.798466615858036
22	22.563762857765692	24.51285396349552	29.206455962944627	23.71692721579416
23	21.675400455925768	26.20069183102019	29.65663618712221	22.467271525931835
24	21.97298580681793	24.818719656348907	31.13983131760519	22.06846321922797
25	21.90302536657412	26.57550404469357	29.099656450301893	22.421814138430417
26	21.19226785047392	27.68321101522062	29.216595157182855	21.9079259771226
27	22.505124517754574	26.25240172163518	29.388623486091557	21.853850274518685
28	20.89637236528813	26.864809053624512	29.793684295909006	22.445134285178355
29	22.089248567416348	26.245304285668418	28.7170708543792	22.948376292536032
30	23.448069581910325	26.580404655242045	28.486404185459378	21.485121577388245
31	24.043071297124015	26.961638358599643	26.72218438800669	22.273105956269657
32	23.97818045399932	27.811471822334276	26.432879379075747	21.777468344590655
33	23.042332825810334	28.47913776292198	26.09338535866555	22.38514405260214
34	22.602460782441618	27.369064980406005	27.56661028148093	22.461863955671443
35	23.50096237851978	27.420436897879725	26.607780479685278	22.470820243915217
36	22.844449551594135	30.775834244452593	25.427916243496128	20.951799960457144
37	23.808348950508904	28.194395391398245	26.362411979120022	21.634843678972832
38	23.526986310397913	28.718760720085573	25.852410508936856	21.901842460579658
39	23.576499375594622	27.472653748206632	26.332163382975953	22.618683493222793
40	23.493864942553017	26.099130902067213	27.896641053935443	22.510363101444327
41	21.471264678595993	27.593817119353524	26.474957035164415	24.459961166886067
42	23.108237588358854	27.86723739064457	26.628565827873658	22.395959193122923
43	23.501131365090416	26.274200989247387	27.783927011320408	22.44074063434179
44	22.962909137610836	26.800932129923634	27.0885472731482	23.14761145931733
45	23.034390456990383	25.188800246044448	27.321241780915674	24.455567516049502
46	23.62736433335643	26.201029804161465	26.474281088881867	23.697324773600243
47	22.082151131449585	26.284002210344344	28.43638416055076	23.197462497655312
48	22.424686910131253	25.806108188582254	28.350201009525772	23.419003891760724
49	22.402887642519048	25.600282545546104	28.57377024247883	23.423059569456015
50	21.66407835569307	25.01237826629918	28.908025679199273	24.415517698808475
51	22.377708643494103	24.251431738719724	27.122851546987558	26.248008070798612
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	224.0
1	445.5
2	667.0
3	4113.5
4	7560.0
5	4974.0
6	2388.0
7	2280.0
8	2172.0
9	2186.5
10	2201.0
11	2202.5
12	2204.0
13	2211.0
14	2218.0
15	2228.0
16	2238.0
17	2057.5
18	1877.0
19	1942.5
20	2008.0
21	2010.5
22	2013.0
23	2190.5
24	2368.0
25	2893.5
26	3945.0
27	4471.0
28	5700.5
29	6930.0
30	7922.0
31	8914.0
32	10126.5
33	11339.0
34	12855.0
35	14371.0
36	15128.0
37	15885.0
38	17837.0
39	19789.0
40	22502.5
41	25216.0
42	28323.5
43	31431.0
44	35206.5
45	38982.0
46	45362.0
47	51742.0
48	56250.0
49	60758.0
50	60187.5
51	59617.0
52	52565.5
53	45514.0
54	40442.5
55	35371.0
56	31755.5
57	28140.0
58	25987.5
59	23835.0
60	22411.0
61	20987.0
62	19353.5
63	17720.0
64	15431.5
65	13143.0
66	10672.5
67	8202.0
68	7026.0
69	5850.0
70	4791.5
71	3733.0
72	3141.0
73	2549.0
74	2031.5
75	1312.5
76	1111.0
77	858.5
78	606.0
79	420.0
80	234.0
81	180.0
82	126.0
83	98.5
84	71.0
85	51.0
86	31.0
87	21.5
88	12.0
89	9.0
90	6.0
91	4.5
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	591763.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.18256788530717
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.21343201197868	27.954768379136972
2	8.758023710094882	6.6880766970365535
3	3.3722693807974964	3.862857136795296
4	2.0697322265910736	3.1611076498488657
5	1.5558055102974477	2.9702324756633636
6	1.3044086382317017	2.988340282767782
7	1.14187140100302	3.051970759956204
8	1.0619787366689066	3.243926016449062
9	0.9351298042882176	3.2135091510427998
>10	6.509988300601771	37.9876834436879
>50	0.05112892032833527	1.3781070712477965
>100	0.02222996535464423	1.6115262903335486
>500	0.002667595842557308	0.7017854929146623
>1k	0.001333797921278654	1.1861091531191934
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4745	0.8018412776736632	No Hit
GCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTCTGC	1238	0.20920537444889253	No Hit
CTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTCTGCT	1004	0.16966251691978038	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTCTGC	970	0.16391697351811452	No Hit
CGCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTCTG	887	0.1498910881552243	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTT	668	0.1128830291856706	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.379731412744629E-4	0.0	0.0	0.19044786510815986	0.0
2	3.379731412744629E-4	0.0	0.0	0.7354295554132313	0.0
3	3.379731412744629E-4	0.0	0.0	1.1388004995243028	0.0
4	3.379731412744629E-4	0.0	0.0	1.5278075851312096	0.0
5	3.379731412744629E-4	0.0	0.0	2.6755643728992857	0.0
6	3.379731412744629E-4	0.0	0.0	3.6757958845010585	0.0
7	3.379731412744629E-4	0.0	0.0	4.549287468124908	0.0
8	3.379731412744629E-4	0.0	0.0	5.993446700790688	0.0
9	3.379731412744629E-4	0.0	0.0	6.657394936824371	0.0
10	3.379731412744629E-4	0.0	0.0	7.761384202797403	0.0
11	3.379731412744629E-4	0.0	0.0	9.15383354484819	0.0
12	3.379731412744629E-4	0.0	0.0	10.216421101015102	0.0
13	3.379731412744629E-4	0.0	0.0	10.698877760184399	0.0
14	3.379731412744629E-4	0.0	0.0	10.93748679792417	0.0
15	3.379731412744629E-4	0.0	0.0	11.279008657182013	0.0
16	3.379731412744629E-4	0.0	0.0	11.946674597769714	0.0
17	3.379731412744629E-4	0.0	0.0	12.769301223631757	0.0
18	3.379731412744629E-4	0.0	0.0	13.618965700795759	0.0
19	3.379731412744629E-4	0.0	0.0	14.142824069771175	0.0
20	3.379731412744629E-4	0.0	0.0	14.654853378801986	0.0
21	3.379731412744629E-4	0.0	0.0	15.288722005262242	0.0
22	3.379731412744629E-4	0.0	0.0	15.934588678237741	0.0
23	5.069597119116944E-4	0.0	0.0	16.593467317152307	0.0
24	5.069597119116944E-4	0.0	0.0	17.140983806016937	0.0
25	8.449328531861573E-4	0.0	0.0	17.62715816974025	0.0
26	8.449328531861573E-4	0.0	0.0	18.097616782394304	0.0
27	8.449328531861573E-4	0.0	0.0	18.556415321674386	0.0
28	8.449328531861573E-4	0.0	0.0	19.03904096741432	0.0
29	8.449328531861573E-4	0.0	0.0	19.541607028489445	0.0
30	8.449328531861573E-4	0.0	0.0	20.131708133154657	0.0
31	0.0010139194238233888	0.0	0.0	20.645258321321204	0.0
32	0.0011829059944606201	0.0	0.0	21.153569925797996	0.0
33	0.0011829059944606201	0.0	0.0	21.63551962525538	0.0
34	0.0011829059944606201	0.0	0.0	22.132002169787565	0.0
35	0.0011829059944606201	0.0	0.0	22.624767009765733	0.0
36	0.0011829059944606201	0.0	0.0	23.120911581156644	0.0
37	0.0011829059944606201	0.0	0.0	23.61198655542844	0.0
38	0.0011829059944606201	0.0	0.0	24.101371663993863	0.0
39	0.0011829059944606201	0.0	0.0	24.616442731296143	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	20	7.030509E-4	45.000004	1
ACGGGTA	20	7.030509E-4	45.000004	5
CGCAGTA	20	7.030509E-4	45.000004	37
CCGAATT	20	7.030509E-4	45.000004	18
CGGTCTA	20	7.030509E-4	45.000004	31
GTACGTT	20	7.030509E-4	45.000004	33
TCGTTTA	25	3.888504E-5	45.0	38
TAATACG	25	3.888504E-5	45.0	1
TAACGCC	25	3.888504E-5	45.0	12
CATCGTT	25	3.888504E-5	45.0	36
GCTACGA	35	1.210592E-7	45.0	10
TAGTACG	25	3.888504E-5	45.0	1
TATAGCG	30	2.1637097E-6	44.999996	1
CGCGACC	65	0.0	41.53846	10
CGTTTTT	2560	0.0	41.39649	1
GTAAGCG	50	1.0804797E-9	40.5	1
TTGCGAG	45	1.9255822E-8	40.0	1
AATACGG	45	1.9255822E-8	40.0	2
TAGGGTA	175	0.0	38.571426	5
ATAACGC	35	6.2431536E-6	38.571426	11
>>END_MODULE