FastQCFastQC Report
Sat 18 Jun 2016
SRR3550487_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550487_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences909373
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52330.575451437418969No Hit
GCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC14060.15461202388898726Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC13410.14746424184575527RNA PCR Primer, Index 30 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGCT11320.12448137342982472RNA PCR Primer, Index 30 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG406.8121153E-945.0000041
GCGGTAT207.0325076E-445.00000416
CGGTCTA351.2115561E-745.031
TGCGACG302.1649994E-644.9999961
CGAATAT1650.043.63636814
CTACGAA1750.041.14285711
CGTTTTT23550.040.4140131
TACGAAT1800.040.012
ATAGACG403.457153E-739.3750041
TCGTAAG403.457153E-739.3750041
GCTACGA1850.038.9189210
CGTAAGG1100.038.8636362
GCACGTT356.2468516E-638.57142610
TATTGCG700.038.5714261
CTAACGG700.038.5714262
CGCACGG950.037.8947372
CGATTAT301.13973445E-437.49999610
TACTAAC301.13973445E-437.49999625
TATGCGG1400.036.9642832
GCGTAAG552.748493E-936.818181
Produced by FastQC (version 0.11.5)