##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550487_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	909373
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29469095739592	33.0	31.0	34.0	30.0	34.0
2	32.40130067640011	34.0	31.0	34.0	30.0	34.0
3	32.47974483517764	34.0	31.0	34.0	30.0	34.0
4	35.99326678931528	37.0	35.0	37.0	35.0	37.0
5	35.96945697749988	37.0	35.0	37.0	35.0	37.0
6	35.979164765173365	37.0	35.0	37.0	35.0	37.0
7	36.137997279444185	37.0	35.0	37.0	35.0	37.0
8	36.11950981610406	37.0	35.0	37.0	35.0	37.0
9	37.659212446377886	39.0	38.0	39.0	35.0	39.0
10	37.44611836946995	39.0	37.0	39.0	35.0	39.0
11	37.41846854920918	39.0	37.0	39.0	35.0	39.0
12	37.326957145197845	39.0	37.0	39.0	35.0	39.0
13	37.27347853960916	39.0	37.0	39.0	34.0	39.0
14	38.488901693804415	40.0	38.0	41.0	34.0	41.0
15	38.53998854155556	40.0	38.0	41.0	34.0	41.0
16	38.554905412850395	40.0	38.0	41.0	34.0	41.0
17	38.57801144304922	40.0	38.0	41.0	34.0	41.0
18	38.51053198192601	40.0	38.0	41.0	34.0	41.0
19	38.427374685635044	40.0	37.0	41.0	34.0	41.0
20	38.307429404655736	40.0	37.0	41.0	34.0	41.0
21	38.250760688958216	40.0	37.0	41.0	34.0	41.0
22	38.231214254216916	40.0	37.0	41.0	34.0	41.0
23	38.19382145720183	40.0	37.0	41.0	34.0	41.0
24	38.15354095624128	40.0	37.0	41.0	34.0	41.0
25	38.104972327086905	40.0	37.0	41.0	34.0	41.0
26	37.98101988952828	40.0	36.0	41.0	34.0	41.0
27	37.92604794732195	40.0	36.0	41.0	34.0	41.0
28	37.85246428033381	40.0	36.0	41.0	33.0	41.0
29	37.78360914608197	40.0	36.0	41.0	33.0	41.0
30	37.73931818956578	40.0	36.0	41.0	33.0	41.0
31	37.60196750948181	40.0	36.0	41.0	33.0	41.0
32	37.4179022249396	40.0	36.0	41.0	33.0	41.0
33	37.28801932760265	40.0	35.0	41.0	32.0	41.0
34	37.07242462663835	40.0	35.0	41.0	31.0	41.0
35	37.0083530080616	40.0	35.0	41.0	31.0	41.0
36	36.877323166621395	39.0	35.0	41.0	31.0	41.0
37	36.826277006244965	39.0	35.0	41.0	31.0	41.0
38	36.74015063125912	39.0	35.0	41.0	31.0	41.0
39	36.623042469921586	39.0	35.0	41.0	31.0	41.0
40	36.50322584901905	39.0	35.0	41.0	30.0	41.0
41	36.37270075095698	39.0	35.0	41.0	30.0	41.0
42	36.33375633540912	39.0	35.0	41.0	30.0	41.0
43	36.27166520228773	39.0	35.0	41.0	30.0	41.0
44	36.07823852258644	39.0	35.0	41.0	30.0	41.0
45	35.998180064725915	39.0	35.0	41.0	29.0	41.0
46	35.86424602445861	38.0	35.0	40.0	29.0	41.0
47	35.749050169732335	38.0	35.0	40.0	28.0	41.0
48	35.63318792178787	38.0	35.0	40.0	28.0	41.0
49	35.550047120378544	38.0	35.0	40.0	28.0	41.0
50	35.46172362715849	38.0	35.0	40.0	28.0	41.0
51	34.20330821346136	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	5.0
11	7.0
12	6.0
13	8.0
14	16.0
15	36.0
16	63.0
17	104.0
18	215.0
19	358.0
20	680.0
21	1110.0
22	1681.0
23	2639.0
24	4030.0
25	6066.0
26	7955.0
27	9614.0
28	10781.0
29	12005.0
30	14392.0
31	17664.0
32	22339.0
33	29555.0
34	52188.0
35	68453.0
36	67898.0
37	100530.0
38	175076.0
39	303682.0
40	213.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.08152210369123	21.194493348713895	24.419682572497752	12.304301975097127
2	34.767471653545904	24.487531518969664	24.38471342342471	16.360283404059718
3	31.91330730074458	24.631696784487772	26.64286271969808	16.81213319506957
4	29.32636003048254	25.858696046616735	25.865623896904786	18.949320025995934
5	25.7529088723769	30.235228008748887	24.181056618131393	19.83080650074282
6	26.619000124261444	34.558536486128354	24.0534962001291	14.768967189481105
7	79.63453940242343	6.52603497134839	9.214810644257089	4.624614981971095
8	80.16138592194842	5.611008903937108	9.413628950936523	4.813976223177948
9	72.49412507298986	8.332664374244672	12.077772267265468	7.095438285500009
10	38.55205729662086	26.314834506852524	19.08347839665352	16.0496297998731
11	30.43107723673344	25.087175449458034	24.92618540466893	19.555561909139595
12	27.307826381473827	23.062813608937148	29.072448819131424	20.5569111904576
13	24.815779663570396	23.88029994292771	30.525757857336867	20.77816253616503
14	21.50492702114534	27.013227795415084	29.859474605030062	21.62237057840952
15	20.906052851800087	25.61908039935208	33.15724130802212	20.31762544082571
16	23.896135029300407	24.53437698282223	31.09791031842819	20.47157766944917
17	23.676423205879217	24.763655837593596	28.26848828808421	23.291432668442983
18	24.331929802182383	24.618610845054782	29.277645146710974	21.77181420605186
19	25.132481391024363	25.762915767237427	27.306836688575537	21.797766153162673
20	27.154643913993485	24.943560013327865	27.29682979371501	20.604966278963637
21	24.76145652004183	26.074339132567165	27.66862442584066	21.49557992155034
22	23.766925123134293	25.15084569258159	26.871261847448736	24.210967336835377
23	23.46968735601343	26.15263483740995	27.697655417523944	22.680022389052677
24	23.839282670587316	24.099571902838548	29.33119852909642	22.729946897477713
25	23.197411843105083	26.020895716059307	27.18191545163536	23.59977698920025
26	22.241038605720647	27.43912563931412	27.853037202556045	22.466798552409188
27	23.160023444725102	26.083466300406982	28.359100171216873	22.397410083651042
28	21.452913161046126	27.0707399493937	28.297299348012313	23.179047541547856
29	22.998153672915294	26.50947411018361	27.49971683786521	22.992655379035885
30	23.688739384169093	25.328660516641687	28.126962203628214	22.855637895561006
31	23.87491161492589	26.65638852264142	26.159782619453182	23.308917242979504
32	24.08307702120032	27.30419750751342	26.280085289534654	22.332640181751604
33	23.850499190101313	26.70235425947329	26.213006104205867	23.234140446219538
34	22.759527718548934	27.448032875398766	26.815399181633936	22.977040224418364
35	23.34718536838019	27.463098200628345	26.239507880704615	22.950208550286845
36	22.682111740726853	28.985355844081585	25.57619370709269	22.756338708098877
37	22.65857904292298	28.281904125149964	26.229610951721682	22.829905880205374
38	23.44582476057679	27.182905144533652	25.415863457569117	23.95540663732044
39	24.216575596592378	26.230600644619972	25.875520825887726	23.67730293289992
40	23.873262126762064	26.028703293368068	27.95090683360953	22.147127746260338
41	20.563509143112892	27.847099045166285	26.980238032138626	24.609153779582197
42	22.298110896188913	26.720168731642573	27.137819134722495	23.843901237446023
43	23.451323054456203	25.143477978783185	27.543813154778075	23.86138581198254
44	23.657838972566815	25.677472280351406	26.538285170111713	24.126403576970066
45	23.49871834769671	24.787738364785408	26.64022353863596	25.07331974888192
46	23.02630493757787	26.136249921649313	26.59557739233516	24.24186774843766
47	22.25753348735887	26.552360802443005	27.91274867408643	23.277357036111695
48	21.984598179185	24.993154624120137	28.12729210126098	24.894955095433886
49	22.42842046113091	24.895394958944237	28.063621858137424	24.61256272178743
50	21.155235530414913	24.671724363929872	28.537464824664905	25.63557528099031
51	21.611703888283465	24.876810725631838	27.0381900496276	26.4732953364571
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	240.0
1	788.5
2	1337.0
3	4952.0
4	8567.0
5	5595.5
6	2624.0
7	2562.5
8	2501.0
9	2514.0
10	2527.0
11	2445.0
12	2363.0
13	2476.0
14	2589.0
15	2571.5
16	2554.0
17	2583.5
18	2613.0
19	2703.0
20	2793.0
21	2896.0
22	2999.0
23	3258.0
24	3517.0
25	4287.5
26	6191.5
27	7325.0
28	8197.5
29	9070.0
30	11109.5
31	13149.0
32	15033.0
33	16917.0
34	19250.5
35	21584.0
36	22638.0
37	23692.0
38	26479.5
39	29267.0
40	32785.5
41	36304.0
42	41111.5
43	45919.0
44	50947.0
45	55975.0
46	63951.0
47	71927.0
48	77853.5
49	83780.0
50	83555.0
51	83330.0
52	75252.0
53	67174.0
54	61228.0
55	55282.0
56	52254.5
57	49227.0
58	46354.5
59	43482.0
60	42024.0
61	40566.0
62	37442.5
63	34319.0
64	30143.0
65	25967.0
66	21982.0
67	17997.0
68	15321.5
69	12646.0
70	10353.0
71	8060.0
72	6904.5
73	5749.0
74	4616.5
75	2944.5
76	2405.0
77	1819.5
78	1234.0
79	955.0
80	676.0
81	488.5
82	301.0
83	234.5
84	168.0
85	117.0
86	66.0
87	49.0
88	32.0
89	20.0
90	8.0
91	6.5
92	5.0
93	3.0
94	1.0
95	1.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	909373.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.631211712754
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.7230777756823	23.730421473013642
2	9.276337168547217	6.053962441315066
3	3.316766096543954	3.2469029009403134
4	1.8493130772998203	2.4138130619414015
5	1.230981047745211	2.0084201591680864
6	0.9699821394838076	1.8991015530651725
7	0.8486209426195614	1.9384070749747042
8	0.767195934955027	2.0027626382945387
9	0.7315439882642455	2.1484050082418724
>10	8.173945286206102	49.22163417074519
>50	0.07052932563485002	1.5614500852918702
>100	0.0386554694312998	2.372933693692248
>500	0.0016954153259323439	0.39357106912515927
>1k	0.0010172491955594064	0.42919937507351497
>5k	3.3908306518646875E-4	0.5790152951172219
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5233	0.575451437418969	No Hit
GCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	1406	0.15461202388898726	Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	1341	0.14746424184575527	RNA PCR Primer, Index 30 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGCT	1132	0.12448137342982472	RNA PCR Primer, Index 30 (96% over 25bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.2989763276455316E-4	0.0	0.0	0.14339550437499243	0.0
2	3.2989763276455316E-4	0.0	0.0	0.5788603796242026	0.0
3	3.2989763276455316E-4	0.0	0.0	0.869500194089774	0.0
4	3.2989763276455316E-4	0.0	0.0	1.2102844487355573	0.0
5	3.2989763276455316E-4	0.0	0.0	2.2499018554542527	0.0
6	3.2989763276455316E-4	0.0	0.0	3.132927852487373	0.0
7	3.2989763276455316E-4	0.0	0.0	3.9154450374048935	0.0
8	3.2989763276455316E-4	0.0	0.0	5.108354877481517	0.0
9	3.2989763276455316E-4	0.0	0.0	5.675668839958961	0.0
10	3.2989763276455316E-4	0.0	0.0	6.6589837173525055	0.0
11	3.2989763276455316E-4	0.0	0.0	7.907426325611163	0.0
12	3.2989763276455316E-4	0.0	0.0	8.838947274660672	0.0
13	3.2989763276455316E-4	0.0	0.0	9.263745459783829	0.0
14	3.2989763276455316E-4	0.0	0.0	9.46234383470809	0.0
15	3.2989763276455316E-4	0.0	0.0	9.796420170820994	0.0
16	3.2989763276455316E-4	0.0	0.0	10.456985197493218	0.0
17	3.2989763276455316E-4	0.0	0.0	11.207392346154988	0.0
18	3.2989763276455316E-4	0.0	0.0	11.979792670334396	0.0
19	3.2989763276455316E-4	0.0	0.0	12.470570382010463	1.0996587758818438E-4
20	5.49829387940922E-4	0.0	0.0	12.94309375800689	1.0996587758818438E-4
21	5.49829387940922E-4	0.0	0.0	13.547906084741905	1.0996587758818438E-4
22	5.49829387940922E-4	0.0	0.0	14.18713773116202	1.0996587758818438E-4
23	5.49829387940922E-4	0.0	0.0	14.823620230642431	1.0996587758818438E-4
24	5.49829387940922E-4	0.0	0.0	15.335181493182665	1.0996587758818438E-4
25	5.49829387940922E-4	0.0	0.0	15.796378383787511	1.0996587758818438E-4
26	5.49829387940922E-4	0.0	0.0	16.221836364176195	1.0996587758818438E-4
27	5.49829387940922E-4	0.0	0.0	16.636297756806062	1.0996587758818438E-4
28	5.49829387940922E-4	0.0	0.0	17.083968844467563	1.0996587758818438E-4
29	5.49829387940922E-4	0.0	0.0	17.532629625027354	1.0996587758818438E-4
30	5.49829387940922E-4	0.0	0.0	18.07992979778375	1.0996587758818438E-4
31	5.49829387940922E-4	0.0	0.0	18.56663877198905	1.0996587758818438E-4
32	7.697611431172907E-4	0.0	0.0	19.05928590358412	1.0996587758818438E-4
33	7.697611431172907E-4	0.0	0.0	19.520152896556198	1.0996587758818438E-4
34	7.697611431172907E-4	0.0	0.0	19.980030196629986	1.0996587758818438E-4
35	7.697611431172907E-4	0.0	0.0	20.46904845426464	1.0996587758818438E-4
36	7.697611431172907E-4	0.0	0.0	20.955647462592356	1.0996587758818438E-4
37	7.697611431172907E-4	0.0	0.0	21.434878757121666	1.0996587758818438E-4
38	7.697611431172907E-4	0.0	0.0	21.918178789121736	1.0996587758818438E-4
39	7.697611431172907E-4	0.0	0.0	22.39773998128381	1.0996587758818438E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	40	6.8121153E-9	45.000004	1
GCGGTAT	20	7.0325076E-4	45.000004	16
CGGTCTA	35	1.2115561E-7	45.0	31
TGCGACG	30	2.1649994E-6	44.999996	1
CGAATAT	165	0.0	43.636368	14
CTACGAA	175	0.0	41.142857	11
CGTTTTT	2355	0.0	40.414013	1
TACGAAT	180	0.0	40.0	12
ATAGACG	40	3.457153E-7	39.375004	1
TCGTAAG	40	3.457153E-7	39.375004	1
GCTACGA	185	0.0	38.91892	10
CGTAAGG	110	0.0	38.863636	2
GCACGTT	35	6.2468516E-6	38.571426	10
TATTGCG	70	0.0	38.571426	1
CTAACGG	70	0.0	38.571426	2
CGCACGG	95	0.0	37.894737	2
CGATTAT	30	1.13973445E-4	37.499996	10
TACTAAC	30	1.13973445E-4	37.499996	25
TATGCGG	140	0.0	36.964283	2
GCGTAAG	55	2.748493E-9	36.81818	1
>>END_MODULE