FastQCFastQC Report
Sat 18 Jun 2016
SRR3550486_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550486_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences670949
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC14750.21983787143285108TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11920.1776588086426837No Hit
CCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC8960.13354219173141327TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGCT7280.10850303078177327TruSeq Adapter, Index 16 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCA207.0311833E-445.00000418
TACGTAG406.8084773E-945.0000041
GGTTACG302.1641463E-644.9999961
GCGTATG302.1641463E-644.9999961
CGTAAGG650.041.538462
TGTTGCG501.0804797E-940.4999961
TACGCGG403.4553523E-739.3750042
ACACGAC3950.039.30379526
AACACGT3650.038.83561741
CGAGACA4250.038.6470622
AAGTACG356.2444014E-638.571431
CGTTGAT356.2444014E-638.5714325
CACGACC4050.038.33333627
GACACGA4150.037.95180525
CGACCAA4100.037.86585229
CGCTACG301.1394155E-437.49999633
CGAATAT301.1394155E-437.49999614
CATACGG301.1394155E-437.4999962
CACGTGA3750.037.19999743
GCGATTA552.744855E-936.818189
Produced by FastQC (version 0.11.5)