##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550486_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	670949
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25534429591519	33.0	31.0	34.0	30.0	34.0
2	32.35523862469427	34.0	31.0	34.0	30.0	34.0
3	32.46559276487483	34.0	31.0	34.0	30.0	34.0
4	35.95530956898364	37.0	35.0	37.0	35.0	37.0
5	35.92738792367229	37.0	35.0	37.0	35.0	37.0
6	35.939263640008406	37.0	35.0	37.0	35.0	37.0
7	36.1126926189621	37.0	35.0	37.0	35.0	37.0
8	36.10124018368013	37.0	35.0	37.0	35.0	37.0
9	37.681947510168435	39.0	38.0	39.0	35.0	39.0
10	37.40375647031294	39.0	37.0	39.0	35.0	39.0
11	37.35531612685912	39.0	37.0	39.0	34.0	39.0
12	37.250363291397704	39.0	37.0	39.0	34.0	39.0
13	37.189079944973464	39.0	37.0	39.0	34.0	39.0
14	38.37416852845745	40.0	38.0	41.0	34.0	41.0
15	38.43725529064057	40.0	38.0	41.0	34.0	41.0
16	38.46581483838563	40.0	38.0	41.0	34.0	41.0
17	38.47706606612425	40.0	38.0	41.0	34.0	41.0
18	38.446686707931605	40.0	38.0	41.0	34.0	41.0
19	38.399477456557804	40.0	37.0	41.0	34.0	41.0
20	38.2955366205181	40.0	37.0	41.0	34.0	41.0
21	38.24592331160789	40.0	37.0	41.0	34.0	41.0
22	38.22434342997754	40.0	37.0	41.0	34.0	41.0
23	38.1598735522372	40.0	37.0	41.0	34.0	41.0
24	38.12667728843772	40.0	37.0	41.0	34.0	41.0
25	38.08785019427706	40.0	37.0	41.0	34.0	41.0
26	37.96446078614023	40.0	37.0	41.0	34.0	41.0
27	37.91289203799395	40.0	36.0	41.0	33.0	41.0
28	37.823144531104454	40.0	36.0	41.0	33.0	41.0
29	37.76611188033666	40.0	36.0	41.0	33.0	41.0
30	37.74423540388316	40.0	36.0	41.0	33.0	41.0
31	37.66237970397154	40.0	36.0	41.0	33.0	41.0
32	37.517546042992834	40.0	36.0	41.0	33.0	41.0
33	37.43745053647893	40.0	36.0	41.0	33.0	41.0
34	37.27812546110062	40.0	35.0	41.0	32.0	41.0
35	37.26288585272502	40.0	35.0	41.0	32.0	41.0
36	37.13085346278182	40.0	35.0	41.0	32.0	41.0
37	37.101720100931665	39.0	35.0	41.0	32.0	41.0
38	36.99061031464389	39.0	35.0	41.0	31.0	41.0
39	36.91933216980724	39.0	35.0	41.0	31.0	41.0
40	36.84422511994205	39.0	35.0	41.0	31.0	41.0
41	36.72326659701408	39.0	35.0	41.0	31.0	41.0
42	36.66292519997794	39.0	35.0	41.0	31.0	41.0
43	36.584731477355206	39.0	35.0	41.0	31.0	41.0
44	36.39113106957459	39.0	35.0	41.0	30.0	41.0
45	36.33086568427704	39.0	35.0	41.0	30.0	41.0
46	36.223157050684925	38.0	35.0	41.0	30.0	41.0
47	36.1215144519181	38.0	35.0	40.0	30.0	41.0
48	36.00110738670152	38.0	35.0	40.0	30.0	41.0
49	35.91411716836898	38.0	35.0	40.0	29.0	41.0
50	35.81942293676568	38.0	35.0	40.0	29.0	41.0
51	34.617498498395555	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	5.0
10	4.0
11	5.0
12	6.0
13	3.0
14	6.0
15	13.0
16	45.0
17	82.0
18	157.0
19	310.0
20	466.0
21	740.0
22	1175.0
23	1725.0
24	2626.0
25	3593.0
26	4734.0
27	5750.0
28	6943.0
29	8596.0
30	10224.0
31	12659.0
32	16523.0
33	22409.0
34	39396.0
35	51511.0
36	50797.0
37	72693.0
38	128106.0
39	229491.0
40	151.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.39957284383761	21.42711294002972	24.507078779460137	10.666235436672535
2	31.987677155789783	23.795847374390604	25.377040579835423	18.839434889984187
3	32.66567205555116	24.65150108279467	25.745622990719113	16.93720387093505
4	28.74898092105361	26.246853337586018	25.056747979354615	19.947417762005756
5	25.64650964529346	30.60828766418908	23.5450086370201	20.20019405349736
6	26.87000055145771	34.87418566835929	22.99280571250572	15.263008067677275
7	81.7865441337568	6.025197146131822	7.620400358298469	4.5678583618128945
8	82.71001223639949	5.0507564658416655	7.5461771311977515	4.693054166561095
9	75.93080845190916	7.487603379690558	10.002995756756475	6.578592411643807
10	40.09604306735683	27.12620482331742	16.761780701662868	16.015971407662878
11	31.31489874789291	25.89615604166636	23.733994685140004	19.054950525300733
12	28.241490783949303	23.78765003003209	27.209221565275456	20.761637620743155
13	25.271369358922957	23.8563586800189	29.80047663831379	21.071795322744354
14	21.907328276813885	26.96419549026826	29.026200202996055	22.102276029921796
15	21.67228805766161	25.622364740092017	32.20244757798283	20.50289962426354
16	23.878715073723935	24.5456808192575	30.413339911081174	21.162264195937396
17	23.67631518938101	24.266225897944555	28.162498192858177	23.89496071981626
18	25.181645698853416	24.457745670684357	27.924029993337797	22.436578637124434
19	24.950778673192747	26.569828705311433	26.191111395948123	22.288281225547692
20	27.196552942175934	25.428907413231112	26.23016056361959	21.14437908097337
21	26.014793970927748	26.842576708512865	26.31720145644453	20.82542786411486
22	24.926335682741907	24.435985447478124	26.056227820594412	24.581451049185556
23	24.054436328245515	27.20653879803085	25.99273566247211	22.746289211251526
24	23.66275231053329	24.724233883648385	28.00406588280182	23.608947923016505
25	23.811943977858228	26.146100523288656	26.235526098108796	23.80642940074432
26	22.62347808849853	27.20788018165315	26.84362000688577	23.32502172296255
27	24.488299408747906	25.596133238144773	26.913520997870183	23.002046355237134
28	21.97320511693139	27.328306622410942	27.435021141696314	23.26346711896135
29	23.348123329791086	25.764551404056046	26.679971204964907	24.207354061187957
30	24.11301007975271	24.45819279855846	27.31310427469152	24.115692846997312
31	24.242826205866617	26.412737778877382	25.281206172153176	24.063229843102828
32	23.964414582926572	26.447017582558434	25.900478277782664	23.68808955673233
33	23.309223204744324	26.013005459431344	26.063978037078822	24.61379329874551
34	22.898312688445767	25.98125938037019	26.606493190987692	24.51393474019635
35	21.96456064469878	25.564983329582425	27.167340587734685	25.303115437984108
36	22.106896351287507	27.41490038736178	26.636897886426542	23.841305374924175
37	23.314737781858234	26.194837461565633	27.191485492936124	23.29893926364001
38	22.24938110050093	26.776997953644766	27.124267269196316	23.849353676657987
39	23.121280454997326	24.575787429446947	26.992066461087205	25.31086565446852
40	22.970300276175983	23.4189185765237	29.277486068240655	24.33329507905966
41	20.514375906365462	25.20832432867476	28.83691606962675	25.44038369533303
42	23.016205404583655	24.748974959348622	28.716787714118357	23.51803192194936
43	23.386725369588447	24.72125303115438	28.501868249300617	23.390153349956556
44	22.743457401382223	25.819548132570436	26.691447487066828	24.745546978980517
45	22.478310572040495	23.896749231312665	27.016509451538045	26.60843074510879
46	22.603059248914597	24.895334816804258	27.3016279925896	25.199977941691543
47	22.099593262677192	25.069267559829434	28.785198278855773	24.045940898637603
48	22.289473566545297	24.286793780153186	28.617525326067998	24.806207327233516
49	22.58040476996016	24.00897832771194	29.014425835644737	24.39619106668316
50	21.111738746164015	23.874243794982927	28.72334558960517	26.290671869247884
51	21.206380812848664	23.522205115440965	26.921718342228694	28.349695729481674
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	190.0
1	455.5
2	721.0
3	1570.0
4	2419.0
5	1728.5
6	1038.0
7	1056.0
8	1074.0
9	1081.0
10	1088.0
11	1153.5
12	1219.0
13	1251.5
14	1284.0
15	1345.0
16	1406.0
17	1410.5
18	1415.0
19	1415.5
20	1416.0
21	1469.5
22	1523.0
23	1817.5
24	2112.0
25	2590.5
26	3906.5
27	4744.0
28	6000.5
29	7257.0
30	8196.5
31	9136.0
32	10065.0
33	10994.0
34	12968.0
35	14942.0
36	16216.0
37	17490.0
38	19550.0
39	21610.0
40	24658.0
41	27706.0
42	31118.0
43	34530.0
44	38128.5
45	41727.0
46	47577.0
47	53427.0
48	58091.0
49	62755.0
50	62435.5
51	62116.0
52	57366.0
53	52616.0
54	47513.0
55	42410.0
56	39571.5
57	36733.0
58	35727.0
59	34721.0
60	33234.0
61	31747.0
62	28944.0
63	26141.0
64	22728.0
65	19315.0
66	16217.5
67	13120.0
68	11076.0
69	9032.0
70	7401.0
71	5770.0
72	5017.5
73	4265.0
74	3559.0
75	2418.0
76	1983.0
77	1474.5
78	966.0
79	709.0
80	452.0
81	338.0
82	224.0
83	165.0
84	106.0
85	75.0
86	44.0
87	32.5
88	21.0
89	14.0
90	7.0
91	9.0
92	11.0
93	6.5
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	670949.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.47746987751102
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.17253179268944	23.439812272807774
2	9.300988020084374	6.041451165067621
3	3.366113111198224	3.279685115197056
4	1.7994039330282894	2.3376034812960444
5	1.2747727401869307	2.070069663504661
6	1.0435813455453726	2.0335729028809353
7	0.8621071340380932	1.959934093383667
8	0.7795297581030272	2.025372338993161
9	0.7176431244996645	2.0976509662866665
>10	8.57916334191259	50.815372389489724
>50	0.07190198672632162	1.5909261726510617
>100	0.030420071302676023	1.66622122936672
>500	9.218203425053339E-4	0.24309974633829426
>1k	9.218203425053339E-4	0.3992284627365953
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	1475	0.21983787143285108	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1192	0.1776588086426837	No Hit
CCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	896	0.13354219173141327	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGCT	728	0.10850303078177327	TruSeq Adapter, Index 16 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.9808524940047604E-4	0.0	0.0	0.12489771949879946	0.0
2	2.9808524940047604E-4	0.0	0.0	0.6613021257949561	0.0
3	2.9808524940047604E-4	0.0	0.0	0.9425455586043052	0.0
4	2.9808524940047604E-4	0.0	0.0	1.2609006049640137	0.0
5	2.9808524940047604E-4	0.0	0.0	2.2810973710371427	0.0
6	2.9808524940047604E-4	0.0	0.0	3.0854804165443275	0.0
7	2.9808524940047604E-4	0.0	0.0	3.8275636449268124	0.0
8	2.9808524940047604E-4	0.0	0.0	5.138691614414807	0.0
9	2.9808524940047604E-4	0.0	0.0	5.711611463762521	0.0
10	2.9808524940047604E-4	0.0	0.0	6.7723478237541155	0.0
11	2.9808524940047604E-4	0.0	0.0	8.019238421996306	0.0
12	2.9808524940047604E-4	0.0	0.0	9.003068787642578	0.0
13	4.471278741007141E-4	0.0	0.0	9.444383999379983	0.0
14	5.961704988009521E-4	0.0	0.0	9.644399201727703	0.0
15	5.961704988009521E-4	0.0	0.0	9.955451159477098	0.0
16	5.961704988009521E-4	0.0	0.0	10.55728527801666	0.0
17	5.961704988009521E-4	0.0	0.0	11.25853082723128	0.0
18	5.961704988009521E-4	0.0	0.0	11.999570757240864	0.0
19	5.961704988009521E-4	0.0	0.0	12.469502152920715	0.0
20	5.961704988009521E-4	0.0	0.0	12.935558440358358	0.0
21	5.961704988009521E-4	0.0	0.0	13.51265148319768	0.0
22	5.961704988009521E-4	0.0	0.0	14.097047614647312	0.0
23	7.452131235011901E-4	0.0	0.0	14.694261411821167	0.0
24	7.452131235011901E-4	0.0	0.0	15.20324197517248	0.0
25	7.452131235011901E-4	0.0	0.0	15.64843229515209	0.0
26	7.452131235011901E-4	0.0	0.0	16.04965504084513	0.0
27	7.452131235011901E-4	0.0	0.0	16.453113425908676	0.0
28	7.452131235011901E-4	0.0	0.0	16.87624543743265	0.0
29	7.452131235011901E-4	0.0	0.0	17.316815436046554	0.0
30	7.452131235011901E-4	0.0	0.0	17.830863448637675	0.0
31	7.452131235011901E-4	0.0	0.0	18.291554201586113	0.0
32	7.452131235011901E-4	0.0	0.0	18.769981026873875	0.0
33	7.452131235011901E-4	0.0	0.0	19.215320389478187	0.0
34	8.942557482014282E-4	0.0	0.0	19.65141910935108	0.0
35	8.942557482014282E-4	0.0	0.0	20.115686885292323	0.0
36	8.942557482014282E-4	0.0	0.0	20.57652668086546	0.0
37	8.942557482014282E-4	0.0	0.0	21.044073394550107	0.0
38	8.942557482014282E-4	0.0	0.0	21.505956488496146	0.0
39	8.942557482014282E-4	0.0	0.0	21.995710553261127	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGCA	20	7.0311833E-4	45.000004	18
TACGTAG	40	6.8084773E-9	45.000004	1
GGTTACG	30	2.1641463E-6	44.999996	1
GCGTATG	30	2.1641463E-6	44.999996	1
CGTAAGG	65	0.0	41.53846	2
TGTTGCG	50	1.0804797E-9	40.499996	1
TACGCGG	40	3.4553523E-7	39.375004	2
ACACGAC	395	0.0	39.303795	26
AACACGT	365	0.0	38.835617	41
CGAGACA	425	0.0	38.64706	22
AAGTACG	35	6.2444014E-6	38.57143	1
CGTTGAT	35	6.2444014E-6	38.57143	25
CACGACC	405	0.0	38.333336	27
GACACGA	415	0.0	37.951805	25
CGACCAA	410	0.0	37.865852	29
CGCTACG	30	1.1394155E-4	37.499996	33
CGAATAT	30	1.1394155E-4	37.499996	14
CATACGG	30	1.1394155E-4	37.499996	2
CACGTGA	375	0.0	37.199997	43
GCGATTA	55	2.744855E-9	36.81818	9
>>END_MODULE