FastQCFastQC Report
Sat 18 Jun 2016
SRR3550484_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550484_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502081
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58341.1619639062223028No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTT13080.26051573351710183No Hit
GCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC12990.25872319406629607TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGCT11900.23701354960653762TruSeq Adapter, Index 27 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTG11140.22187654979973348TruSeq Adapter, Index 27 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC10840.21590141829704768TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCT9800.19518762908773685No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTC9720.19359426068702063No Hit
CGTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCT6810.13563548511096815No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCC5490.10934490649915053No Hit
TCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC5300.1055606565474495TruSeq Adapter, Index 20 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC207.029486E-445.00000425
CAGGTCG207.029486E-445.0000041
TACGGGT253.887657E-545.04
AACGATG253.887657E-545.01
GGACGCA253.887657E-545.08
TTACGCG253.887657E-545.01
GTACGAG351.2101009E-745.01
TATTGCG302.1630494E-644.9999961
CGTTTTT37200.044.032261
CGTTCTG1300.043.269231
GCTAGCG700.041.7857131
CGCCGGT556.002665E-1140.90909228
CGTTTCT1600.040.7812541
TACGCGG451.9246727E-840.0000042
ATGACGG451.9246727E-840.0000042
TTCACGG403.4530422E-739.3750042
GCTCGAG403.4530422E-739.3750041
ACGACTG403.4530422E-739.3750041
CGATAGG750.039.02
CGAACGG700.038.5714262
Produced by FastQC (version 0.11.5)