FastQCFastQC Report
Sat 18 Jun 2016
SRR3550482_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550482_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences582970
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGC19140.3283187814124226No Hit
CCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGC12050.20670017325076762No Hit
CTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGCT11290.19366348182582294No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10540.1807983258143644No Hit
TCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGC6530.11201262500643257No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCG351.2105556E-745.0000041
GCGATAC351.2105556E-745.0000049
TGTTACG207.0304226E-445.01
GCGATAT253.888432E-545.09
CTACCGC207.0304226E-445.020
CGGTCGT207.0304226E-445.024
GCTACGA603.6379788E-1241.25000410
GTTACGG501.0804797E-940.52
ATTGCGG1250.039.6000022
ACACCGC403.4543154E-739.37533
ACGTGCG403.4543154E-739.3751
CACCGCA403.4543154E-739.37534
TATTGCG700.038.571431
ACGTAGG659.094947E-1238.076922
CGAATAT601.546141E-1037.50000414
GACGAGC301.13923175E-437.5000049
CGGTAGT601.546141E-1037.50000412
TACGTGT301.13923175E-437.50000415
ACACGAC900.037.526
CACGACC900.037.527