##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550482_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	582970
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17855978866837	33.0	31.0	34.0	30.0	34.0
2	32.28904060243237	34.0	31.0	34.0	30.0	34.0
3	32.3933821637477	34.0	31.0	34.0	30.0	34.0
4	35.898603701734224	37.0	35.0	37.0	35.0	37.0
5	35.868017222155515	37.0	35.0	37.0	35.0	37.0
6	35.8901195601832	37.0	35.0	37.0	35.0	37.0
7	36.073671029384016	37.0	35.0	37.0	35.0	37.0
8	36.05635796010086	37.0	35.0	37.0	35.0	37.0
9	37.63430708269722	39.0	38.0	39.0	35.0	39.0
10	37.38302142477315	39.0	37.0	39.0	34.0	39.0
11	37.33643755253272	39.0	37.0	39.0	34.0	39.0
12	37.22358097329194	39.0	37.0	39.0	34.0	39.0
13	37.12718493232928	39.0	37.0	39.0	33.0	39.0
14	38.290529529821434	40.0	37.0	41.0	34.0	41.0
15	38.35210902790881	40.0	38.0	41.0	34.0	41.0
16	38.37363500694718	40.0	38.0	41.0	34.0	41.0
17	38.39637031065063	40.0	37.0	41.0	34.0	41.0
18	38.35768221349298	40.0	37.0	41.0	34.0	41.0
19	38.33567421994271	40.0	37.0	41.0	34.0	41.0
20	38.22170780657667	40.0	37.0	41.0	34.0	41.0
21	38.177954268658766	40.0	37.0	41.0	34.0	41.0
22	38.15728939739609	40.0	37.0	41.0	34.0	41.0
23	38.12923821122871	40.0	37.0	41.0	34.0	41.0
24	38.10612381426145	40.0	37.0	41.0	34.0	41.0
25	38.05503027600048	40.0	37.0	41.0	34.0	41.0
26	37.921227507418905	40.0	36.0	41.0	34.0	41.0
27	37.873592123093815	40.0	36.0	41.0	33.0	41.0
28	37.792330651663036	40.0	36.0	41.0	33.0	41.0
29	37.74517899720397	40.0	36.0	41.0	33.0	41.0
30	37.72754172598933	40.0	36.0	41.0	33.0	41.0
31	37.59729317117519	40.0	36.0	41.0	33.0	41.0
32	37.46274765425322	40.0	36.0	41.0	33.0	41.0
33	37.36501706777364	40.0	35.0	41.0	32.0	41.0
34	37.16965367000017	39.0	35.0	41.0	32.0	41.0
35	37.13527625778342	39.0	35.0	41.0	32.0	41.0
36	37.028116369624506	39.0	35.0	41.0	31.0	41.0
37	36.93797279448342	39.0	35.0	41.0	31.0	41.0
38	36.84590802271129	39.0	35.0	41.0	31.0	41.0
39	36.77394033998319	39.0	35.0	41.0	31.0	41.0
40	36.6622690704496	39.0	35.0	41.0	31.0	41.0
41	36.57996637905896	39.0	35.0	41.0	31.0	41.0
42	36.48807314270031	39.0	35.0	41.0	30.0	41.0
43	36.369334614131084	39.0	35.0	41.0	30.0	41.0
44	36.16820591111035	39.0	35.0	41.0	30.0	41.0
45	36.17497126781824	39.0	35.0	40.0	30.0	41.0
46	36.10579446626756	38.0	35.0	40.0	30.0	41.0
47	36.019863800881694	38.0	35.0	40.0	29.0	41.0
48	35.87247885826029	38.0	35.0	40.0	29.0	41.0
49	35.75541623068082	38.0	35.0	40.0	28.0	41.0
50	35.650542909583685	38.0	35.0	40.0	28.0	41.0
51	34.35006261042592	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	5.0
11	3.0
12	8.0
13	3.0
14	6.0
15	17.0
16	29.0
17	65.0
18	103.0
19	196.0
20	417.0
21	633.0
22	988.0
23	1542.0
24	2213.0
25	3125.0
26	4260.0
27	5297.0
28	6427.0
29	7745.0
30	9365.0
31	11587.0
32	14994.0
33	20237.0
34	35846.0
35	47960.0
36	43695.0
37	64819.0
38	112826.0
39	188414.0
40	143.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.784534367120095	21.268847453556784	25.567181844691838	10.379436334631285
2	32.60871056829682	23.923701048081377	25.871657203629688	17.595931179992107
3	32.20097089044033	24.508979878896	26.492958471276395	16.797090759387277
4	29.03374101583272	25.913340309106815	25.84112390002916	19.211794775031304
5	24.98653447004134	30.63519563613908	24.349451944353913	20.028817949465665
6	26.900355078305914	34.52458960152324	23.565535104722372	15.009520215448479
7	81.07895775082767	6.175446420913597	7.949808738013962	4.795787090244781
8	81.44501432320702	5.413657649621764	7.89697583066024	5.244352196510969
9	74.42767209290359	8.084292502187077	10.83829356570664	6.6497418392027035
10	39.22568914352368	28.95912311096626	17.16331886718013	14.651868878329932
11	29.942707171895638	24.6263101017205	25.80664528191845	19.624337444465407
12	28.006586959877865	22.282964818086697	29.662075235432354	20.048372986603084
13	24.53625400964029	23.989570646860045	31.5491363191931	19.925039024306567
14	21.200576358989313	27.000703295195294	30.080107038098014	21.718613307717376
15	20.535190490076676	25.300101205893956	34.61601797691134	19.548690327118035
16	23.542892430142203	24.926325539907715	30.48681750347359	21.04396452647649
17	22.997409815256358	24.807108427534867	27.984630426951647	24.21085133025713
18	23.34185292553648	24.862171295263906	30.08919841501278	21.706777364186834
19	23.855773024340877	26.794346192771496	27.867643274954112	21.48223750793351
20	25.845926891606773	25.868569566186938	27.841912962931197	20.443590579275092
21	24.376383004271233	26.582156886289177	28.09732919361202	20.94413091582757
22	22.606823678748476	25.689315059093946	26.958848654304678	24.745012607852892
23	21.769559325522756	26.596737396435493	27.99406487469338	23.63963840334837
24	23.376331543647186	24.403314064188553	29.52604765253787	22.694306739626395
25	22.556735338010533	25.737859581110524	27.46693654905055	24.238468531828396
26	21.710894214110503	26.937578263032403	28.159596548707484	23.191930974149614
27	22.25174537283222	26.674786009571676	28.18138154622022	22.892087071375887
28	20.51220474466954	27.000531759781808	28.88055989158962	23.606703603959037
29	22.87630581333516	26.037360413057275	27.97948436454706	23.1068494090605
30	23.819579052095303	26.198089095493764	26.93500523183011	23.04732662058082
31	24.290615297528177	27.79697068459783	24.75787776386435	23.154536254009642
32	24.621164039315918	29.138892224299706	24.26128274182205	21.978660994562325
33	23.637751513800023	28.686038732696368	25.041254266943408	22.6349554865602
34	23.225380379779406	27.871073983223837	26.170128823095528	22.73341681390123
35	23.31131962193595	28.471447930425235	25.13920098804398	23.078031459594833
36	23.995745921745545	29.428958608504725	24.462322246427775	22.112973223321955
37	23.156423143557987	30.156954903339795	25.427208947287166	21.259413005815052
38	21.682076264644838	30.773281643995404	24.79595862565827	22.748683465701493
39	24.539684717910013	27.447896118153594	26.097226272363926	21.915192891572467
40	24.460606892292912	26.36962450897988	27.216323309947338	21.953445288779868
41	22.40990102406642	26.931574523560386	25.888296138737843	24.77022831363535
42	23.64358371785855	27.0019040430897	26.04422182959672	23.310290409455032
43	23.523165857591298	25.747122493438766	26.987495068356864	23.742216580613068
44	21.971284971782424	27.09521930802614	26.243545980067584	24.689949740123847
45	22.549016244403656	26.245261334202446	26.070123677033123	25.13559874436077
46	23.34614131087363	26.80446678216718	26.502221383604645	23.347170523354546
47	22.126696056400842	26.45693603444431	28.296653344082884	23.11971456507196
48	21.935948676604287	25.32600305333036	27.8604387875877	24.87760948247766
49	22.851776249206647	24.808480710842755	28.872326191742285	23.467416848208313
50	21.740741376057088	24.503833816491415	28.252054136576497	25.503370670875004
51	21.139509751788257	25.090141859786954	26.617836252294286	27.152512136130504
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	206.0
1	532.0
2	858.0
3	1483.0
4	2108.0
5	1489.0
6	870.0
7	896.0
8	922.0
9	993.5
10	1065.0
11	1045.5
12	1026.0
13	1113.0
14	1200.0
15	1184.0
16	1168.0
17	1238.0
18	1308.0
19	1379.0
20	1450.0
21	1620.0
22	1790.0
23	1916.0
24	2042.0
25	2441.0
26	3614.0
27	4388.0
28	5601.5
29	6815.0
30	7832.0
31	8849.0
32	9436.0
33	10023.0
34	12062.5
35	14102.0
36	14991.5
37	15881.0
38	17440.0
39	18999.0
40	22370.5
41	25742.0
42	29008.0
43	32274.0
44	36059.5
45	39845.0
46	46027.5
47	52210.0
48	57534.0
49	62858.0
50	61346.5
51	59835.0
52	52235.0
53	44635.0
54	39301.0
55	33967.0
56	31260.5
57	28554.0
58	27028.5
59	25503.0
60	24541.0
61	23579.0
62	21486.5
63	19394.0
64	16152.0
65	12910.0
66	10970.0
67	9030.0
68	7197.0
69	5364.0
70	4562.0
71	3760.0
72	2994.0
73	2228.0
74	1859.5
75	1290.5
76	1090.0
77	764.0
78	438.0
79	262.5
80	87.0
81	116.5
82	146.0
83	102.5
84	59.0
85	47.0
86	35.0
87	24.0
88	13.0
89	9.0
90	5.0
91	3.5
92	2.0
93	3.0
94	4.0
95	2.0
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	582970.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.231366380547975
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.16872003114287	23.982651765701
2	10.556936964677988	7.016428802591284
3	3.6950180308376135	3.6837149389648687
4	1.8708317041315252	2.486811751853585
5	1.199496359237746	1.993045149298146
6	0.8592214336197862	1.7131861357583278
7	0.7326063527210438	1.7041857083992973
8	0.6353138025540689	1.68898765914347
9	0.5980993785024581	1.78880936210939
>10	7.5639580830869395	49.11408264889111
>50	0.08453268872962373	1.8710918833177295
>100	0.03215353804437956	1.8400707412890307
>500	0.001037210904657405	0.2031884802828292
>1k	0.00207442180931481	0.9137449723999664
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGC	1914	0.3283187814124226	No Hit
CCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGC	1205	0.20670017325076762	No Hit
CTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGCT	1129	0.19366348182582294	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1054	0.1807983258143644	No Hit
TCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGC	653	0.11201262500643257	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.146062404583426E-4	0.0	0.0	0.220766077156629	0.0
2	5.146062404583426E-4	0.0	0.0	1.0533989742182273	0.0
3	5.146062404583426E-4	0.0	0.0	1.4686862102681097	0.0
4	5.146062404583426E-4	0.0	0.0	1.9513868638180352	0.0
5	5.146062404583426E-4	0.0	0.0	3.319038715542824	0.0
6	5.146062404583426E-4	0.0	0.0	4.173456610117158	0.0
7	5.146062404583426E-4	0.0	0.0	5.092200284748786	0.0
8	5.146062404583426E-4	0.0	0.0	6.647511878827384	0.0
9	5.146062404583426E-4	0.0	0.0	7.27052850060895	0.0
10	5.146062404583426E-4	0.0	0.0	8.517934027479972	0.0
11	5.146062404583426E-4	0.0	0.0	10.183542892430141	0.0
12	5.146062404583426E-4	0.0	0.0	11.46645624989279	0.0
13	5.146062404583426E-4	0.0	0.0	11.992383827641216	0.0
14	6.861416539444569E-4	0.0	0.0	12.201142425853817	0.0
15	6.861416539444569E-4	0.0	0.0	12.593443916496561	0.0
16	6.861416539444569E-4	0.0	0.0	13.439971182050535	0.0
17	6.861416539444569E-4	0.0	0.0	14.425270597114775	0.0
18	6.861416539444569E-4	0.0	0.0	15.444534024049265	0.0
19	6.861416539444569E-4	0.0	0.0	16.089507178757053	0.0
20	6.861416539444569E-4	0.0	0.0	16.747173954062816	0.0
21	6.861416539444569E-4	0.0	0.0	17.500729025507315	0.0
22	6.861416539444569E-4	0.0	0.0	18.328558930991303	0.0
23	6.861416539444569E-4	0.0	0.0	19.090519237696622	0.0
24	6.861416539444569E-4	0.0	0.0	19.724685661354787	0.0
25	6.861416539444569E-4	0.0	0.0	20.30687685472666	0.0
26	6.861416539444569E-4	0.0	0.0	20.837607424052695	0.0
27	6.861416539444569E-4	0.0	0.0	21.35735972691562	0.0
28	6.861416539444569E-4	0.0	0.0	21.896152460675506	0.0
29	6.861416539444569E-4	0.0	0.0	22.476799835326002	0.0
30	6.861416539444569E-4	0.0	0.0	23.102561023723347	0.0
31	6.861416539444569E-4	0.0	0.0	23.672058596497248	0.0
32	8.57677067430571E-4	0.0	0.0	24.248074514983617	0.0
33	0.0010292124809166852	0.0	0.0	24.79904626310102	0.0
34	0.0010292124809166852	0.0	0.0	25.355850215276945	0.0
35	0.0010292124809166852	0.0	0.0	25.909394994596635	0.0
36	0.0012007478944027995	0.0	0.0	26.457965246925227	0.0
37	0.0012007478944027995	0.0	0.0	27.03123659879582	0.0
38	0.0012007478944027995	0.0	0.0	27.60433641525293	0.0
39	0.0012007478944027995	0.0	0.0	28.174863200507744	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATGCG	35	1.2105556E-7	45.000004	1
GCGATAC	35	1.2105556E-7	45.000004	9
TGTTACG	20	7.0304226E-4	45.0	1
GCGATAT	25	3.888432E-5	45.0	9
CTACCGC	20	7.0304226E-4	45.0	20
CGGTCGT	20	7.0304226E-4	45.0	24
GCTACGA	60	3.6379788E-12	41.250004	10
GTTACGG	50	1.0804797E-9	40.5	2
ATTGCGG	125	0.0	39.600002	2
ACACCGC	40	3.4543154E-7	39.375	33
ACGTGCG	40	3.4543154E-7	39.375	1
CACCGCA	40	3.4543154E-7	39.375	34
TATTGCG	70	0.0	38.57143	1
ACGTAGG	65	9.094947E-12	38.07692	2
CGAATAT	60	1.546141E-10	37.500004	14
GACGAGC	30	1.13923175E-4	37.500004	9
CGGTAGT	60	1.546141E-10	37.500004	12
TACGTGT	30	1.13923175E-4	37.500004	15
ACACGAC	90	0.0	37.5	26
CACGACC	90	0.0	37.5	27
>>END_MODULE