FastQCFastQC Report
Sat 18 Jun 2016
SRR3550481_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550481_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences770231
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33580.4359731041726443No Hit
GCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC18600.24148599575971363No Hit
CCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC13460.17475276897450245No Hit
CTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGCT12720.1651452616163203No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT9740.12645556982255973No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGG302.1645646E-645.0000042
GCCGATA351.2112287E-745.09
ACGTAAC253.8896032E-545.026
TCGACCC207.031836E-445.036
ACGTAGG1250.039.6000022
CGCATGG1200.039.3750042
TGTAACG403.4562436E-739.3751
CGATACG403.4562436E-739.37510
TACGTAG403.4562436E-739.3751
CGTTTTT20650.039.2251851
AACGTAG301.1395721E-437.5000041
ACGATAG301.1395721E-437.5000041
TGTACCG301.1395721E-437.5000041
GGTTACG301.1395721E-437.5000041
CTTACGT301.1395721E-437.50000422
GCTACGA2000.037.12510
TACGAAT1950.036.9230812
CGAATAT1950.036.9230814
CACGACC4450.036.91011427
GCGAGAC4600.036.6847821
Produced by FastQC (version 0.11.5)