##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550481_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	770231
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.20706385486951	33.0	31.0	34.0	30.0	34.0
2	32.316202541834855	34.0	31.0	34.0	30.0	34.0
3	32.41104551751358	34.0	31.0	34.0	30.0	34.0
4	35.92247260886669	37.0	35.0	37.0	35.0	37.0
5	35.90132960111966	37.0	35.0	37.0	35.0	37.0
6	35.91324550686742	37.0	35.0	37.0	35.0	37.0
7	36.10285485782837	37.0	35.0	37.0	35.0	37.0
8	36.080758889216355	37.0	35.0	37.0	35.0	37.0
9	37.674680712669314	39.0	38.0	39.0	35.0	39.0
10	37.39767550254404	39.0	37.0	39.0	34.0	39.0
11	37.36105791639132	39.0	37.0	39.0	34.0	39.0
12	37.28267104284299	39.0	37.0	39.0	34.0	39.0
13	37.230145242141646	39.0	37.0	39.0	34.0	39.0
14	38.40789321645065	40.0	38.0	41.0	34.0	41.0
15	38.46435420023344	40.0	38.0	41.0	34.0	41.0
16	38.496669181063865	40.0	38.0	41.0	34.0	41.0
17	38.48859108501216	40.0	38.0	41.0	34.0	41.0
18	38.43469816197998	40.0	38.0	41.0	34.0	41.0
19	38.35915588959676	40.0	37.0	41.0	34.0	41.0
20	38.23955021285822	40.0	37.0	41.0	34.0	41.0
21	38.200097113723025	40.0	37.0	41.0	34.0	41.0
22	38.1774441693466	40.0	37.0	41.0	34.0	41.0
23	38.1407577726682	40.0	37.0	41.0	34.0	41.0
24	38.1057578830247	40.0	37.0	41.0	34.0	41.0
25	38.07376358521015	40.0	37.0	41.0	34.0	41.0
26	37.94418427718438	40.0	36.0	41.0	34.0	41.0
27	37.906807178625634	40.0	36.0	41.0	33.0	41.0
28	37.818284384814426	40.0	36.0	41.0	33.0	41.0
29	37.75682619889358	40.0	36.0	41.0	33.0	41.0
30	37.70658542696931	40.0	36.0	41.0	33.0	41.0
31	37.59104476449273	40.0	36.0	41.0	33.0	41.0
32	37.39929190074147	40.0	36.0	41.0	33.0	41.0
33	37.25109739805331	40.0	35.0	41.0	32.0	41.0
34	37.06491688856979	40.0	35.0	41.0	31.0	41.0
35	37.00202666472786	40.0	35.0	41.0	31.0	41.0
36	36.84682906816267	39.0	35.0	41.0	31.0	41.0
37	36.81589419278113	39.0	35.0	41.0	31.0	41.0
38	36.690944664652555	39.0	35.0	41.0	31.0	41.0
39	36.57916261485191	39.0	35.0	41.0	30.0	41.0
40	36.4745861955699	39.0	35.0	41.0	30.0	41.0
41	36.340605610524634	39.0	35.0	41.0	30.0	41.0
42	36.26700171766652	39.0	35.0	41.0	30.0	41.0
43	36.18436936451532	39.0	35.0	41.0	30.0	41.0
44	35.98774783149471	39.0	35.0	41.0	29.0	41.0
45	35.900146839065165	38.0	35.0	40.0	29.0	41.0
46	35.748149581099696	38.0	35.0	40.0	28.0	41.0
47	35.627529922841326	38.0	35.0	40.0	28.0	41.0
48	35.509713319770306	38.0	35.0	40.0	27.0	41.0
49	35.41092996776292	38.0	34.0	40.0	27.0	41.0
50	35.31304764414831	38.0	34.0	40.0	27.0	41.0
51	33.984481278992924	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	6.0
11	6.0
12	9.0
13	11.0
14	11.0
15	23.0
16	49.0
17	86.0
18	148.0
19	293.0
20	511.0
21	927.0
22	1510.0
23	2264.0
24	3428.0
25	5157.0
26	6906.0
27	8224.0
28	9374.0
29	10690.0
30	12741.0
31	15378.0
32	19783.0
33	26309.0
34	45026.0
35	59449.0
36	58300.0
37	84877.0
38	149669.0
39	248867.0
40	196.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.96633087995679	20.829465446080462	24.542507377656833	11.661696296305914
2	33.47008884347682	23.7791000362229	24.40644430047609	18.34436681982418
3	32.10361566854619	24.29037522509481	26.665506841454057	16.940502264904943
4	27.800101527983163	26.411037727642743	26.142417015155196	19.646443729218895
5	25.19607754037425	30.294158505695044	24.937713491147463	19.572050462783245
6	25.358106853658185	34.22479749581619	25.118827987967247	15.298267662558377
7	80.33044632064926	5.758272518244527	9.157512486513786	4.753768674592427
8	80.49208614039165	5.250113277705	9.084287700702776	5.173512881200574
9	73.76475369077589	7.399598302327484	11.85436576819162	6.981282238705012
10	37.58755490236046	27.6183378752608	18.563651683715666	16.230455538663076
11	29.164497404025546	25.348888839841553	25.836015429137493	19.650598326995407
12	26.418438104932157	23.255361054021456	29.444283598037472	20.881917243008914
13	24.26168253420078	24.419946743249753	31.215570393816922	20.10280032873255
14	21.11379573141045	26.83195041487554	30.00593328494958	22.048320568764435
15	20.483984674727452	25.24450457070671	33.34519124782046	20.92631950674538
16	23.718598706102455	24.433968510745476	30.65794547350081	21.189487309651263
17	22.348100764575822	25.468333525916254	28.700610596041965	23.482955113465962
18	24.36788443986285	23.997995406572834	29.695506932335885	21.938613221228437
19	23.358187348990107	27.58795737902006	27.004496053781267	22.049359218208565
20	26.035955447132093	26.157217769734014	27.347769695065505	20.459057088068384
21	23.714444108325942	27.962909828350195	27.100441296182577	21.222204767141285
22	23.139551641001205	25.02457055091265	27.145882209363165	24.68999559872298
23	22.293831331120145	27.163928743454886	27.500840656893843	23.04139926853113
24	22.60101190422094	24.886560006024165	29.936473603373532	22.57595448638136
25	22.29175403223189	26.728734626365338	27.400091660813448	23.579419680589332
26	21.78073850572101	26.771578915935606	28.247110282499666	23.200572295843713
27	22.486241140644818	25.50507575000227	27.71545159828675	24.29323151106616
28	19.950378522806794	27.088626658755622	28.25321234798392	24.70778247045367
29	21.860714512918854	25.034437720631864	28.339679914207554	24.765167852241728
30	23.038023657837712	25.261122961812752	28.176092626757427	23.524760753592105
31	23.455171240835544	26.449987081797538	25.701510326122946	24.393331351243976
32	23.48970633485279	26.044004980324086	26.407532285768813	24.05875639905431
33	22.41976757622064	27.284671741334744	25.06845348992705	25.22710719251757
34	21.81657191154342	26.673556374646047	27.286619209042485	24.223252504768052
35	22.276433952931004	26.627466305562876	27.140818793323042	23.955280948183077
36	21.18273608826443	30.66469669488764	24.91096826796117	23.241598948886764
37	23.17395690383794	28.45276287243697	25.26722502729701	23.106055196428084
38	21.97262899052362	29.610986833819986	25.36641604921121	23.049968126445183
39	23.849208873701524	27.592111976796573	25.55778720929176	23.000891940210145
40	24.437084459077862	26.373776178834664	27.61846770644131	21.570671655646162
41	20.617061634756325	27.617169394636154	26.706922988038656	25.05884598256887
42	21.881876995342957	27.444883418091454	26.638242293545705	24.034997293019885
43	22.428985590037275	25.412116624752834	27.717788559536032	24.441109225673856
44	22.510909064942854	25.593620615114165	26.874924535626327	25.020545784316656
45	23.139551641001205	24.337763605983138	26.847919650079	25.674765102936654
46	22.956749338834715	26.031800849355584	26.567484300164494	24.443965511645207
47	21.399554159726108	26.565017507734694	28.31994557476913	23.715482757770072
48	21.21792033818426	25.32123479839165	28.407321959256375	25.053522904167714
49	22.766416828198295	24.598334785278702	28.274894155130088	24.360354231392918
50	20.819598276361248	24.356718958338472	29.11217543827761	25.71150732702267
51	21.074456883714106	23.638363036543584	27.36932167103116	27.917858408711155
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	235.0
1	596.5
2	958.0
3	3264.0
4	5570.0
5	3713.5
6	1857.0
7	1879.0
8	1901.0
9	1927.5
10	1954.0
11	2013.5
12	2073.0
13	2062.0
14	2051.0
15	2022.0
16	1993.0
17	1922.0
18	1851.0
19	2081.5
20	2312.0
21	2420.0
22	2528.0
23	2723.5
24	2919.0
25	3166.0
26	4381.5
27	5350.0
28	6634.0
29	7918.0
30	9153.5
31	10389.0
32	11797.0
33	13205.0
34	15439.0
35	17673.0
36	19595.5
37	21518.0
38	23755.5
39	25993.0
40	28375.0
41	30757.0
42	33862.5
43	36968.0
44	42267.0
45	47566.0
46	55923.0
47	64280.0
48	71650.0
49	79020.0
50	79782.5
51	80545.0
52	71852.5
53	63160.0
54	56701.0
55	50242.0
56	45446.0
57	40650.0
58	37743.0
59	34836.0
60	32530.0
61	30224.0
62	27691.0
63	25158.0
64	21046.0
65	16934.0
66	14872.0
67	12810.0
68	10439.0
69	8068.0
70	6629.0
71	5190.0
72	4484.5
73	3779.0
74	3351.0
75	2341.0
76	1759.0
77	1277.5
78	796.0
79	655.5
80	515.0
81	363.0
82	211.0
83	143.5
84	76.0
85	67.5
86	59.0
87	41.5
88	24.0
89	16.5
90	9.0
91	6.0
92	3.0
93	3.0
94	3.0
95	3.5
96	4.0
97	2.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	770231.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.29494379314883
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.39585975162252	26.638986043349743
2	9.688683912149058	7.033004760420708
3	3.4775411077638583	3.786514771339613
4	1.9390201379041603	2.815065076760608
5	1.2846416973161543	2.3312999099212566
6	1.0435692704050326	2.272577280816479
7	0.8798021243123973	2.2352658055707986
8	0.7766568084195348	2.2550972166522696
9	0.694449130785381	2.2684492952150817
>10	6.718199476492609	43.17107166162852
>50	0.06496594362902278	1.6317953970637233
>100	0.03373921651789514	2.1896228033745238
>500	0.0014357113411869868	0.35043344485229483
>1k	0.0014357113411869868	1.0208165330344172
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3358	0.4359731041726443	No Hit
GCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	1860	0.24148599575971363	No Hit
CCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	1346	0.17475276897450245	No Hit
CTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGCT	1272	0.1651452616163203	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	974	0.12645556982255973	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.596623610319502E-4	0.0	0.0	0.1933186277882869	0.0
2	2.596623610319502E-4	0.0	0.0	0.823129684471282	0.0
3	2.596623610319502E-4	0.0	0.0	1.214570693726947	0.0
4	2.596623610319502E-4	0.0	0.0	1.66703235782512	0.0
5	2.596623610319502E-4	0.0	0.0	2.901077728629463	0.0
6	2.596623610319502E-4	0.0	0.0	3.978806358092572	0.0
7	2.596623610319502E-4	0.0	0.0	4.958382615085604	0.0
8	2.596623610319502E-4	0.0	0.0	6.640994714572641	0.0
9	2.596623610319502E-4	0.0	0.0	7.43647035759402	0.0
10	2.596623610319502E-4	0.0	0.0	8.814628338771096	0.0
11	2.596623610319502E-4	0.0	0.0	10.442321848899876	0.0
12	2.596623610319502E-4	0.0	0.0	11.667538699429132	0.0
13	2.596623610319502E-4	0.0	0.0	12.251000024667924	0.0
14	2.596623610319502E-4	0.0	0.0	12.517024113545157	0.0
15	2.596623610319502E-4	0.0	0.0	12.929757436405442	0.0
16	3.8949354154792524E-4	0.0	0.0	13.697189544435371	0.0
17	5.193247220639004E-4	0.0	0.0	14.634051343038648	0.0
18	5.193247220639004E-4	0.0	0.0	15.545466230260791	0.0
19	5.193247220639004E-4	0.0	0.0	16.139573712301893	0.0
20	5.193247220639004E-4	0.0	0.0	16.7264106482341	0.0
21	5.193247220639004E-4	0.0	0.0	17.453075765582014	0.0
22	5.193247220639004E-4	0.0	0.0	18.21804108118214	0.0
23	5.193247220639004E-4	0.0	0.0	19.00313022976224	0.0
24	5.193247220639004E-4	0.0	0.0	19.648001703385088	0.0
25	5.193247220639004E-4	0.0	0.0	20.206275779603782	0.0
26	6.491559025798754E-4	0.0	0.0	20.703269538618933	0.0
27	6.491559025798754E-4	0.0	0.0	21.218958987628387	0.0
28	6.491559025798754E-4	0.0	0.0	21.74178915156622	0.0
29	6.491559025798754E-4	0.0	0.0	22.31200769639238	0.0
30	6.491559025798754E-4	0.0	0.0	22.968304313900635	0.0
31	6.491559025798754E-4	0.0	0.0	23.530862819076354	0.0
32	7.789870830958505E-4	0.0	0.0	24.101600688624583	0.0
33	7.789870830958505E-4	0.0	0.0	24.63299971047647	0.0
34	7.789870830958505E-4	0.0	0.0	25.159465147468747	0.0
35	7.789870830958505E-4	0.0	0.0	25.728515211670267	0.0
36	7.789870830958505E-4	0.0	0.0	26.27471498810097	0.0
37	7.789870830958505E-4	0.0	0.0	26.81572151731104	0.0
38	7.789870830958505E-4	0.0	0.0	27.383992594429465	0.0
39	7.789870830958505E-4	0.0	0.0	27.972387504527862	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCGG	30	2.1645646E-6	45.000004	2
GCCGATA	35	1.2112287E-7	45.0	9
ACGTAAC	25	3.8896032E-5	45.0	26
TCGACCC	20	7.031836E-4	45.0	36
ACGTAGG	125	0.0	39.600002	2
CGCATGG	120	0.0	39.375004	2
TGTAACG	40	3.4562436E-7	39.375	1
CGATACG	40	3.4562436E-7	39.375	10
TACGTAG	40	3.4562436E-7	39.375	1
CGTTTTT	2065	0.0	39.225185	1
AACGTAG	30	1.1395721E-4	37.500004	1
ACGATAG	30	1.1395721E-4	37.500004	1
TGTACCG	30	1.1395721E-4	37.500004	1
GGTTACG	30	1.1395721E-4	37.500004	1
CTTACGT	30	1.1395721E-4	37.500004	22
GCTACGA	200	0.0	37.125	10
TACGAAT	195	0.0	36.92308	12
CGAATAT	195	0.0	36.92308	14
CACGACC	445	0.0	36.910114	27
GCGAGAC	460	0.0	36.68478	21
>>END_MODULE