SRR3550478_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550478_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	474194
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	1641	0.34606089490799125	RNA PCR Primer, Index 3 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	1441	0.303884064328102	RNA PCR Primer, Index 24 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGCT	1139	0.24019705015246923	TruSeq Adapter, Index 3 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	827	0.17440119444784202	No Hit
TCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	548	0.11556451578889652	RNA PCR Primer, Index 24 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGC	508	0.10712914967291867	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCG	493	0.103965887379427	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGTG	55	1.8189894E-12	45.000004	42
TGCGTAG	30	2.162793E-6	45.000004	1
CGATTCG	20	7.02909E-4	45.0	10
CTAAGCG	20	7.02909E-4	45.0	1
TGCGACG	40	6.8012014E-9	45.0	1
GTCAACG	20	7.02909E-4	45.0	1
CGCACGG	50	2.1827873E-11	45.0	2
TCGAACT	20	7.02909E-4	45.0	33
TACGTAG	25	3.887328E-5	45.0	1
ACAACGA	130	0.0	41.538464	13
CACAACG	130	0.0	41.538464	12
CACGACC	60	3.6379788E-12	41.250004	27
TTGCGAG	50	1.0786607E-9	40.5	1
CGGCTAC	50	1.0786607E-9	40.5	25
CGTTCGG	95	0.0	40.263157	45
CGGTAGT	95	0.0	40.263157	12
CGACGGG	135	0.0	40.0	3
TACGAAT	90	0.0	40.0	12
ATGGGCG	155	0.0	39.193546	5
TATGCGG	75	0.0	39.0	2