##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550478_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	474194
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25249792279109	33.0	31.0	34.0	30.0	34.0
2	32.35932972580842	34.0	31.0	34.0	30.0	34.0
3	32.45837357705918	34.0	31.0	34.0	30.0	34.0
4	35.96677309286916	37.0	35.0	37.0	35.0	37.0
5	35.932500200339945	37.0	35.0	37.0	35.0	37.0
6	35.941642028368136	37.0	35.0	37.0	35.0	37.0
7	36.10529234870116	37.0	35.0	37.0	35.0	37.0
8	36.08261175805683	37.0	35.0	37.0	35.0	37.0
9	37.6301260665466	39.0	38.0	39.0	35.0	39.0
10	37.43997604356023	39.0	37.0	39.0	35.0	39.0
11	37.40467403636487	39.0	37.0	39.0	35.0	39.0
12	37.30313753442684	39.0	37.0	39.0	34.0	39.0
13	37.21854346533276	39.0	37.0	39.0	34.0	39.0
14	38.38970547919206	40.0	38.0	41.0	34.0	41.0
15	38.47411818791465	40.0	38.0	41.0	34.0	41.0
16	38.50469639008507	40.0	38.0	41.0	34.0	41.0
17	38.533988198922806	40.0	38.0	41.0	34.0	41.0
18	38.47766104168336	40.0	38.0	41.0	34.0	41.0
19	38.44914317768677	40.0	37.0	41.0	34.0	41.0
20	38.32882744193305	40.0	37.0	41.0	34.0	41.0
21	38.287561630893684	40.0	37.0	41.0	34.0	41.0
22	38.24448010729785	40.0	37.0	41.0	34.0	41.0
23	38.21431102038406	40.0	37.0	41.0	34.0	41.0
24	38.18524485758993	40.0	37.0	41.0	34.0	41.0
25	38.16450440115227	40.0	37.0	41.0	34.0	41.0
26	38.03720418225452	40.0	37.0	41.0	34.0	41.0
27	37.96453982968996	40.0	37.0	41.0	34.0	41.0
28	37.86215768229881	40.0	36.0	41.0	33.0	41.0
29	37.819856430068704	40.0	36.0	41.0	33.0	41.0
30	37.818500444965565	40.0	36.0	41.0	33.0	41.0
31	37.70052974099208	40.0	36.0	41.0	33.0	41.0
32	37.577063395994045	40.0	36.0	41.0	33.0	41.0
33	37.487167699296066	40.0	36.0	41.0	33.0	41.0
34	37.30264828319211	40.0	35.0	41.0	32.0	41.0
35	37.320860238636506	40.0	35.0	41.0	32.0	41.0
36	37.185375605764726	40.0	35.0	41.0	32.0	41.0
37	37.12726436859176	39.0	35.0	41.0	32.0	41.0
38	37.045949548075264	39.0	35.0	41.0	32.0	41.0
39	36.97205784974082	39.0	35.0	41.0	31.0	41.0
40	36.86821849285314	39.0	35.0	41.0	31.0	41.0
41	36.745656840871035	39.0	35.0	41.0	31.0	41.0
42	36.69838294031557	39.0	35.0	41.0	31.0	41.0
43	36.613244790107004	39.0	35.0	41.0	31.0	41.0
44	36.43914305115628	39.0	35.0	41.0	30.0	41.0
45	36.39206949054606	39.0	35.0	41.0	30.0	41.0
46	36.30017039439554	39.0	35.0	41.0	30.0	41.0
47	36.19156927333539	38.0	35.0	40.0	30.0	41.0
48	36.07090979641244	38.0	35.0	40.0	30.0	41.0
49	36.001961222621965	38.0	35.0	40.0	30.0	41.0
50	35.911964301530595	38.0	35.0	40.0	30.0	41.0
51	34.60270269130356	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	5.0
11	7.0
12	5.0
13	4.0
14	11.0
15	19.0
16	27.0
17	49.0
18	92.0
19	207.0
20	314.0
21	535.0
22	784.0
23	1185.0
24	1735.0
25	2482.0
26	3209.0
27	4007.0
28	4636.0
29	5644.0
30	6997.0
31	8750.0
32	11360.0
33	15654.0
34	27309.0
35	37406.0
36	35241.0
37	52847.0
38	91396.0
39	162161.0
40	113.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.48917531643167	20.37647038975609	24.43303795492984	10.701316338882398
2	32.92070334082675	24.186303496037485	24.110596085146586	18.782397077989177
3	32.24903731384202	24.54670451334264	25.622424577282715	17.581833595532633
4	29.327870027878884	25.75148567885718	25.0197176682961	19.90092662496784
5	25.90395492140348	29.78211449322429	23.687351590277398	20.626578995094835
6	26.86326693294306	35.08057883482288	23.086120870361075	14.97003336187299
7	80.09148154552777	6.224878425285853	8.434100811060452	5.249539218125915
8	79.27535987380693	6.3762932470676565	8.647304689641793	5.70104218948363
9	72.20673395277039	8.508121148728158	11.386900719958499	7.89824417854296
10	39.54689430908025	26.6563895789487	18.068132452118753	15.728583659852296
11	30.690392539762207	25.417445180664455	24.035943095020183	19.856219184553158
12	28.43203414636204	22.797209580888836	28.687203971370366	20.08355230137876
13	25.481764847298784	24.669860858635918	29.457352897759144	20.39102139630615
14	21.152945840731853	28.42423143270476	28.128360966186833	22.294461760376556
15	20.17191276144363	25.778900618734102	33.52594085964816	20.523245760174106
16	22.389148745028407	25.116724378629847	31.05922892318334	21.434897953158412
17	22.530441127471036	25.149622306482154	27.65471515877468	24.665221407272128
18	23.384521946713793	25.097112152410194	29.11276819192145	22.405597708954563
19	25.073493127285456	25.469955334736415	26.996123949269705	22.460427588708416
20	27.411987498787415	24.888125956886846	27.019742974394447	20.680143569931296
21	25.210778710822996	26.411553077432444	27.26985157973319	21.10781663201137
22	23.46191643082789	25.101329835468185	26.299151823937038	25.13760190976689
23	23.55892314116163	27.058123890222145	26.22998182178602	23.1529711468302
24	23.81198412464097	24.195582398765062	27.911782941159103	24.080650535434863
25	22.160128554979607	26.016777943204676	27.059389195139545	24.76370430667617
26	22.59012134274158	26.760988118786827	27.6836062877219	22.965284250749693
27	24.539323567991158	26.655756926490003	26.95816480174781	21.84675470377103
28	21.135864224347	26.635512047811655	28.68952369705226	23.539100030789086
29	23.10320248674593	25.52225460465548	28.058136543271317	23.31640636532727
30	25.39677853368031	24.046487302665152	27.146062581981212	23.410671581673324
31	23.77739912356546	26.657865768018997	26.26393417040283	23.30080093801271
32	25.092894469352206	25.851022999025712	27.35420524089297	21.70187729072911
33	25.346166336984442	25.62073750405952	25.504329451659025	23.528766707297013
34	22.499230272841917	25.946975288594963	28.926557484911235	22.62723695365188
35	23.92965748195886	24.095201541984927	28.104109288603397	23.871031687452813
36	24.414691033627587	26.65385896911391	26.459634664293517	22.47181533296499
37	24.548391586565835	27.13024627051376	27.563191436416318	20.75817070650409
38	23.69768491376947	25.901635195721585	26.631294364753664	23.769385525755283
39	24.697908450971543	25.07370401143836	26.406913626068658	23.821473911521444
40	24.305453042425672	23.741970585878352	29.593584060532187	22.358992311163785
41	21.505544144379726	25.359241154464208	28.337979814168886	24.797234886987184
42	24.059773004297817	24.33856185443089	28.779360346187428	22.82230479508387
43	25.23608480917093	23.246392826564655	28.7527889429221	22.76473342134232
44	23.562086403455126	24.201698039199147	27.79516400460571	24.441051552740017
45	23.41151511828492	23.21012075226595	27.04863410334167	26.329730026107455
46	23.264950632019808	24.987452392902483	27.67496003745303	24.072636937624686
47	22.821039490166473	24.522663719912103	29.99194422535924	22.664352564562186
48	22.717284486939946	23.346351915038994	29.350856400544924	24.58550719747614
49	23.661834607776562	22.60846826404383	29.875325288805847	23.85437183937376
50	21.687326284179047	23.39274642867687	29.125421241095417	25.794506046048664
51	21.799727537674453	23.18059697086003	27.346402527235686	27.673272964229827
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	167.0
1	451.5
2	736.0
3	1223.5
4	1711.0
5	1267.5
6	824.0
7	813.0
8	802.0
9	872.0
10	942.0
11	999.0
12	1056.0
13	1053.5
14	1051.0
15	983.0
16	915.0
17	937.5
18	960.0
19	1049.0
20	1138.0
21	1195.5
22	1253.0
23	1296.0
24	1339.0
25	1794.0
26	2762.5
27	3276.0
28	4111.0
29	4946.0
30	5504.0
31	6062.0
32	6859.5
33	7657.0
34	8836.5
35	10016.0
36	11286.5
37	12557.0
38	13416.0
39	14275.0
40	17072.0
41	19869.0
42	21976.5
43	24084.0
44	27225.5
45	30367.0
46	35586.5
47	40806.0
48	44413.0
49	48020.0
50	47659.5
51	47299.0
52	41684.0
53	36069.0
54	32720.0
55	29371.0
56	26860.5
57	24350.0
58	23276.5
59	22203.0
60	21231.5
61	20260.0
62	19273.0
63	18286.0
64	15891.0
65	13496.0
66	11185.5
67	8875.0
68	7510.0
69	6145.0
70	4963.0
71	3781.0
72	3248.0
73	2715.0
74	2390.0
75	1614.0
76	1163.0
77	887.0
78	611.0
79	407.0
80	203.0
81	173.0
82	143.0
83	96.5
84	50.0
85	35.0
86	20.0
87	12.5
88	5.0
89	3.5
90	2.0
91	1.5
92	1.0
93	1.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	474194.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.382409877995855
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.03969570194919	24.048586494085644
2	10.20245713103629	6.8116521142187025
3	3.6427180837967104	3.648081244298683
4	1.864133355058668	2.4891705490324814
5	1.276748433648708	2.1310469761575184
6	0.9467923521910707	1.896372622211648
7	0.7593889697375812	1.7745163691226247
8	0.7122809589264599	1.9022123935340012
9	0.6404186096319079	1.9240844868205715
>10	7.794515773061472	47.75627045083535
>50	0.0809889045190187	1.8954804321134344
>100	0.03606537154362552	2.433244325601378
>500	0.001898177449664501	0.3977256132905725
>1k	0.001898177449664501	0.8915559286773799
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	1641	0.34606089490799125	RNA PCR Primer, Index 3 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	1441	0.303884064328102	RNA PCR Primer, Index 24 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGCT	1139	0.24019705015246923	TruSeq Adapter, Index 3 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	827	0.17440119444784202	No Hit
TCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	548	0.11556451578889652	RNA PCR Primer, Index 24 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGC	508	0.10712914967291867	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCG	493	0.103965887379427	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1088415289944622E-4	0.0	0.0	0.26592491680620167	0.0
2	2.1088415289944622E-4	0.0	0.0	1.1855907076006866	0.0
3	2.1088415289944622E-4	0.0	0.0	1.634773953276507	0.0
4	2.1088415289944622E-4	0.0	0.0	2.145324487446066	0.0
5	2.1088415289944622E-4	0.0	0.0	3.5616224583187472	0.0
6	2.1088415289944622E-4	0.0	0.0	4.3916624841309675	0.0
7	2.1088415289944622E-4	0.0	0.0	5.243634461844731	0.0
8	2.1088415289944622E-4	0.0	0.0	6.654238560589126	0.0
9	2.1088415289944622E-4	0.0	0.0	7.234591749368402	0.0
10	2.1088415289944622E-4	0.0	0.0	8.443379713788028	0.0
11	2.1088415289944622E-4	0.0	0.0	10.003500676938131	0.0
12	2.1088415289944622E-4	0.0	0.0	11.185717238092426	0.0
13	2.1088415289944622E-4	0.0	0.0	11.664846033479968	0.0
14	2.1088415289944622E-4	0.0	0.0	11.86539686288734	0.0
15	2.1088415289944622E-4	0.0	0.0	12.267131174160786	0.0
16	2.1088415289944622E-4	0.0	0.0	13.092320864456319	0.0
17	4.2176830579889244E-4	0.0	0.0	14.049102266161107	0.0
18	8.435366115977849E-4	0.0	0.0	15.016216991357968	0.0
19	8.435366115977849E-4	0.0	0.0	15.599944326583634	0.0
20	8.435366115977849E-4	0.0	0.0	16.166800929577345	0.0
21	8.435366115977849E-4	0.0	0.0	16.875582567472385	0.0
22	8.435366115977849E-4	0.0	0.0	17.61873832229003	0.0
23	8.435366115977849E-4	0.0	0.0	18.3538804792975	0.0
24	8.435366115977849E-4	0.0	0.0	18.94562141233335	0.0
25	8.435366115977849E-4	0.0	0.0	19.479580087474748	0.0
26	8.435366115977849E-4	0.0	0.0	19.968831322201464	0.0
27	8.435366115977849E-4	0.0	0.0	20.481279813747115	0.0
28	8.435366115977849E-4	0.0	0.0	20.968422206944837	0.0
29	8.435366115977849E-4	0.0	0.0	21.477707436197	0.0
30	8.435366115977849E-4	0.0	0.0	22.083155839171308	0.0
31	8.435366115977849E-4	0.0	0.0	22.61648186185401	0.0
32	8.435366115977849E-4	0.0	0.0	23.159930323875884	0.0
33	8.435366115977849E-4	0.0	0.0	23.65255570504899	0.0
34	8.435366115977849E-4	0.0	0.0	24.157412367090263	0.0
35	8.435366115977849E-4	0.0	0.0	24.6755547307642	0.0
36	8.435366115977849E-4	0.0	0.0	25.214996393880984	0.0
37	8.435366115977849E-4	0.0	0.0	25.733771410013624	0.0
38	8.435366115977849E-4	0.0	0.0	26.242845755112885	0.0
39	8.435366115977849E-4	0.0	0.0	26.783763607299967	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGTG	55	1.8189894E-12	45.000004	42
TGCGTAG	30	2.162793E-6	45.000004	1
CGATTCG	20	7.02909E-4	45.0	10
CTAAGCG	20	7.02909E-4	45.0	1
TGCGACG	40	6.8012014E-9	45.0	1
GTCAACG	20	7.02909E-4	45.0	1
CGCACGG	50	2.1827873E-11	45.0	2
TCGAACT	20	7.02909E-4	45.0	33
TACGTAG	25	3.887328E-5	45.0	1
ACAACGA	130	0.0	41.538464	13
CACAACG	130	0.0	41.538464	12
CACGACC	60	3.6379788E-12	41.250004	27
TTGCGAG	50	1.0786607E-9	40.5	1
CGGCTAC	50	1.0786607E-9	40.5	25
CGTTCGG	95	0.0	40.263157	45
CGGTAGT	95	0.0	40.263157	12
CGACGGG	135	0.0	40.0	3
TACGAAT	90	0.0	40.0	12
ATGGGCG	155	0.0	39.193546	5
TATGCGG	75	0.0	39.0	2
>>END_MODULE