FastQCFastQC Report
Sat 18 Jun 2016
SRR3550477_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550477_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences530650
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGC12180.22952982191651747No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10790.20333553189484593No Hit
CTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGCT8000.15075850372185057No Hit
CCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGC7110.13398662018279467No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAAGCG351.2102828E-745.0000041
TGTCGCG207.029849E-445.0000041
TACACGG351.2102828E-745.0000042
GCGTTAG302.163284E-644.9999961
CTAACGG302.163284E-644.9999962
TGTTACG302.163284E-644.9999961
TTGTGCG556.002665E-1140.9090921
GCGATAT900.040.0000049
GCGATGC1750.039.8571439
GCGAGAC1300.039.80769321
GCGCGAC750.039.0000049
TCGTTGA356.241933E-638.5714324
CGAGACA1350.038.33333622
CGAATAT659.094947E-1238.07692714
CACGTGA1300.038.07692743
GTAGGGC1900.037.8947374
AGCGTTG301.1390939E-437.4999961
TTAGGCG301.1390939E-437.4999961
CGTTGAT301.1390939E-437.49999625
GTTAGCG301.1390939E-437.4999961
Produced by FastQC (version 0.11.5)